CAJ | 학술논문

目的探讨血管内皮生长因子C(VEGF-C)和nm23-H1蛋白在Ⅱ期和Ⅲ期结直肠癌组织中的表达水平及其临床意义.方法采用免疫组化法检测110例Ⅱ期和Ⅲ期结直肠癌患者肿瘤组织中VEGF-C和nm23-H1蛋白的表达水平,以53例癌旁组织作为对照；分析VEGF-C和nm23-H1蛋白的表达与Ⅱ、Ⅲ期结直肠癌患者临床病理特征及预后的关系.结果 VEGF-C蛋白在结直肠癌组织中的阻性表达率为71.8％,明显高于癌旁正常组织(22.6％,P＜0.001).nm23-H1蛋白在结直肠癌组织中的阳性表达率57.3％,明显低于癌旁正常组织(90.6％,P＜0.001).VEGF-C蛋白在结直肠癌组织中的表达与淋巴结转移有关(P＜0.001),nm23-H1蛋白在结直肠癌组织中的表达与病理学类型和淋巴结转移有关(均P＜0.05).VEGF-C和nm23-H1蛋白的表达与结直肠癌患者的性别、年龄、肿瘤原发部位、肿瘤大小和浸润深度均无关(均P ＞0.05).在结直肠癌组织中,VEGF-C蛋白与nm23-H1蛋白的表达呈负相关(r=-0.361,P＜0.001).110例结直肠癌患者的中位生存时间为55个月,中位无病生存时间为48个月.VEGF-C和nm23-H1蛋白不同表达状态结直肠癌患者的5年总生存率和5年无病生存率的差异均有统计学意义(均P＜0.001),VEGF-C蛋白阴性/nm23-H1蛋白阳性患者的预后较好.结论 VEGF-C和nm23-H1蛋白表达水平的联合检测对预测Ⅱ、Ⅲ期结直肠癌患者的预后具有重要的临床意义,但是否可作为判断结直肠癌患者预后的标志物仍需进一步研究.
목적 탐토혈관내피생장인자C(VEGF-C)화nm23-H1단백재Ⅱ기화Ⅲ기결직장암조직중적표체수평급기림상의의.방법 채용면역조화법검측110례Ⅱ기화Ⅲ기결직장암환자종류조직중VEGF-C화nm23-H1단백적표체수평,이53례암방조직작위대조；분석VEGF-C화nm23-H1단백적표체여Ⅱ、Ⅲ기결직장암환자림상병리특정급예후적관계.결과 VEGF-C단백재결직장암조직중적조성표체솔위71.8％,명현고우암방정상조직(22.6％,P＜0.001).nm23-H1단백재결직장암조직중적양성표체솔57.3％,명현저우암방정상조직(90.6％,P＜0.001).VEGF-C단백재결직장암조직중적표체여림파결전이유관(P＜0.001),nm23-H1단백재결직장암조직중적표체여병이학류형화림파결전이유관(균P＜0.05).VEGF-C화nm23-H1단백적표체여결직장암환자적성별、년령、종류원발부위、종류대소화침윤심도균무관(균P ＞0.05).재결직장암조직중,VEGF-C단백여nm23-H1단백적표체정부상관(r=-0.361,P＜0.001).110례결직장암환자적중위생존시간위55개월,중위무병생존시간위48개월.VEGF-C화nm23-H1단백불동표체상태결직장암환자적5년총생존솔화5년무병생존솔적차이균유통계학의의(균P＜0.001),VEGF-C단백음성/nm23-H1단백양성환자적예후교호.결론 VEGF-C화nm23-H1단백표체수평적연합검측대예측Ⅱ、Ⅲ기결직장암환자적예후구유중요적림상의의,단시부가작위판단결직장암환자예후적표지물잉수진일보연구.
Objective To discuss the expression and clinical significance of VEGF-C and nm23-H1 in stage Ⅱ and Ⅲ colorectal carcinomas.Methods SP immunohistochemical staining was employed to determine the expression of vascular endothelial growth factor-C (VEGF-C) and nm23-H1 in the tumor tissues of 110 cases of stage Ⅱ and Ⅲl colorectal carcinomas and in the adjacent mucosal tissues of 53 cases as control,and analyze their correlation with cliniopathological features and prognosis.Results The positive expression of VEGF-C in the carcinoma tissues was 71.8％,significantly higher than that in the adjacent mucosal tissues (22.6％,P ＜ 0.001).The positive expression of nm23-H1 in the carcinoma tissues was 57.3％,significantly lower than that in the adjacent mucosal tissues (90.6％,P ＜0.001).The expression of VEGF-C was significantly correlated with lymph node metastasis (P ＜ 0.05),and the nm23-H1 expression was significantly correlated with lymph node metastasis and pathological type (P ＜ 0.05).The expression of VEGF-C and nm23-H1 did not show a significant correlation with age,gender,primary tumor site,tumor size and depth of invasion (P ＞ 0.05).The VEGF-C expression was negatively related with nm23-H1 expression in colorectal carcinoma (r =-0.361,P ＜ 0.001).The median overall survival (MOS) and median disease free survival (MDFS) of 110 patients with colorectal carcinoma were 55 and 48 months,respectively.The colorectal patients with different VEGF-C and nm23-H1 expression showed significant differences in the 5-year OS rate and 5-year DFS rate (P ＜ 0.001).The patients with negative VEGF-C expression and positive nm23-H1 expression had a better prognosis.Conclusions The joint detection of VEGF-C and nm23-H1 expression is very promising in prediction of the prognosis of patients with stage Ⅱ and Ⅲ colorectal carcinoma.However,whether it can be used as a marker in prognosis judgment needs further investigation.