中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2013年
11期
828-832
,共5页
刘秉乾%王义昆%武玉东%魏金星%李鑫
劉秉乾%王義昆%武玉東%魏金星%李鑫
류병건%왕의곤%무옥동%위금성%리흠
前列腺肿瘤%前列腺癌抗原1%LNCaP细胞%细胞增殖%细胞周期
前列腺腫瘤%前列腺癌抗原1%LNCaP細胞%細胞增殖%細胞週期
전렬선종류%전렬선암항원1%LNCaP세포%세포증식%세포주기
Prostatic neoplasms%Prostate cancer antigen-1%LNCaP cells%Cell proliferation%Cell cycle
目的 探讨前列腺癌抗原1(PCA-1)蛋白表达下调对前列腺癌LNCaP细胞增殖、细胞周期分布和细胞侵袭能力的影响,及其可能的分子机制.方法 将PCA-1-siRNA和对照siRNA转染前列腺癌LNCaP细胞,采用四甲基偶氮唑蓝(MTT)法、流式细胞术和Transwell侵袭实验检测前列腺癌LNCaP细胞增殖、细胞周期分布和细胞侵袭能力,采用Western blot法检测前列腺癌LNCaP细胞中Bcl-xl、细胞周期素E(cyclin E)、基质金属蛋白酶9(MMP-9)和p21蛋白的表达水平.采用免疫组织化学法检测126例前列腺癌组织和88例良性前列腺增生(BPH)组织中PCA-1蛋白的表达情况.结果 重组质粒PCA-1-siRNA可明显抑制前列腺癌LNCaP细胞中PCA-1蛋白的表达,转染48 h后,未转染组、对照组和实验组LNCaP细胞中PCA-1蛋白的相对表达量分别为0.816±0.078、0.799±0.081和0.226-±0.013.PCA-1蛋白表达下调能明显抑制前列腺癌LNCaP细胞增殖,将细胞周期阻滞在S期,并降低细胞的侵袭能力.PCA-1蛋白表达下调能明显降低LNCaP细胞中Bcl-xl、cyclin E和MMP-9的表达,同时提高p21的表达.PCA-1蛋白在前列腺癌组织和BPH组织中的阳性表达率分别为77.8％ (98/126)和10.2％ (9/88),差异有统计学意义(P＜0.05).PCA-1蛋白表达与前列腺癌患者的Gleason评分、临床分期和骨转移情况有关(均P＜0.05).结论 PCA-1蛋白表达可能在前列腺癌的发展中具有重要作用,下调PCA-1表达可导致前列腺癌LNCaP细胞增殖、细胞周期分布和侵袭能力的改变,这可能与Bcl-xl、cyclin E、MMP-9和p21蛋白的表达变化相关.
目的 探討前列腺癌抗原1(PCA-1)蛋白錶達下調對前列腺癌LNCaP細胞增殖、細胞週期分佈和細胞侵襲能力的影響,及其可能的分子機製.方法 將PCA-1-siRNA和對照siRNA轉染前列腺癌LNCaP細胞,採用四甲基偶氮唑藍(MTT)法、流式細胞術和Transwell侵襲實驗檢測前列腺癌LNCaP細胞增殖、細胞週期分佈和細胞侵襲能力,採用Western blot法檢測前列腺癌LNCaP細胞中Bcl-xl、細胞週期素E(cyclin E)、基質金屬蛋白酶9(MMP-9)和p21蛋白的錶達水平.採用免疫組織化學法檢測126例前列腺癌組織和88例良性前列腺增生(BPH)組織中PCA-1蛋白的錶達情況.結果 重組質粒PCA-1-siRNA可明顯抑製前列腺癌LNCaP細胞中PCA-1蛋白的錶達,轉染48 h後,未轉染組、對照組和實驗組LNCaP細胞中PCA-1蛋白的相對錶達量分彆為0.816±0.078、0.799±0.081和0.226-±0.013.PCA-1蛋白錶達下調能明顯抑製前列腺癌LNCaP細胞增殖,將細胞週期阻滯在S期,併降低細胞的侵襲能力.PCA-1蛋白錶達下調能明顯降低LNCaP細胞中Bcl-xl、cyclin E和MMP-9的錶達,同時提高p21的錶達.PCA-1蛋白在前列腺癌組織和BPH組織中的暘性錶達率分彆為77.8％ (98/126)和10.2％ (9/88),差異有統計學意義(P＜0.05).PCA-1蛋白錶達與前列腺癌患者的Gleason評分、臨床分期和骨轉移情況有關(均P＜0.05).結論 PCA-1蛋白錶達可能在前列腺癌的髮展中具有重要作用,下調PCA-1錶達可導緻前列腺癌LNCaP細胞增殖、細胞週期分佈和侵襲能力的改變,這可能與Bcl-xl、cyclin E、MMP-9和p21蛋白的錶達變化相關.
목적 탐토전렬선암항원1(PCA-1)단백표체하조대전렬선암LNCaP세포증식、세포주기분포화세포침습능력적영향,급기가능적분자궤제.방법 장PCA-1-siRNA화대조siRNA전염전렬선암LNCaP세포,채용사갑기우담서람(MTT)법、류식세포술화Transwell침습실험검측전렬선암LNCaP세포증식、세포주기분포화세포침습능력,채용Western blot법검측전렬선암LNCaP세포중Bcl-xl、세포주기소E(cyclin E)、기질금속단백매9(MMP-9)화p21단백적표체수평.채용면역조직화학법검측126례전렬선암조직화88례량성전렬선증생(BPH)조직중PCA-1단백적표체정황.결과 중조질립PCA-1-siRNA가명현억제전렬선암LNCaP세포중PCA-1단백적표체,전염48 h후,미전염조、대조조화실험조LNCaP세포중PCA-1단백적상대표체량분별위0.816±0.078、0.799±0.081화0.226-±0.013.PCA-1단백표체하조능명현억제전렬선암LNCaP세포증식,장세포주기조체재S기,병강저세포적침습능력.PCA-1단백표체하조능명현강저LNCaP세포중Bcl-xl、cyclin E화MMP-9적표체,동시제고p21적표체.PCA-1단백재전렬선암조직화BPH조직중적양성표체솔분별위77.8％ (98/126)화10.2％ (9/88),차이유통계학의의(P＜0.05).PCA-1단백표체여전렬선암환자적Gleason평분、림상분기화골전이정황유관(균P＜0.05).결론 PCA-1단백표체가능재전렬선암적발전중구유중요작용,하조PCA-1표체가도치전렬선암LNCaP세포증식、세포주기분포화침습능력적개변,저가능여Bcl-xl、cyclin E、MMP-9화p21단백적표체변화상관.
Objective To detect the expression of prostate cancer antigen-1 (PCA-1) in prostate cancer,and to analyze the effects of downregulation of PCA-1 expression on malignant biological behavior of prostate cancer LNCaP cells,and to explore their possible molecular mechanisms.Methods PCA-1-siRNA and control siRNA were transfected into LNCaP cells with lipofectamine 2000.The cell cycle,proliferation and migration were determined by methyl thiazolyl tetrazolium (MTT) assay,flow cytometry and Transwell chambers,respectively.Western blotting was used to detect the expression of cyclin E,matrix metallopeptidase 9 (MMP-9)and p21.Immunohistochemistry was used to detect the expression of PCA-1 protein in 126 cases of prostate cancer and 88 cases of benign prostatic hyperplasia (BPH).Results The positive rate of PCA-1 expression was 77.8％ (98/126) in prostate cancer,and 10.2％ (9/88)in BPH,and its expression was not significantly related to age,prostate specific antigen (PSA),Eastern Cooperative Oncology Group (ECOG) score (P ＞ 0.05),and was associated with Gleason score,TNM staging and bone metastasis (P ＜ 0.05).Downregulation of PCA-1 expression inhibited cell proliferation,arrested cell cycle at S phase and decreased cell migration of LNCaP cells.The downregulation of PCA-1 expression decreased the expression of Bcl-xl,cyclin E and MMP-9 proteins,but increased the expression of p21 proteins.Conclusions PCA-1 may play an important role in the development of prostate cancer.The downregulation of PCA-1 expression can lead to changes in the proliferation,cell cycle and migration of prostate cancer LNCaP cells,and these effects may be associated with the decrease of Bcl-xl,cyclin E and MMP-9 proteins and increase of p21 protein.
