CAJ | 학술논문

目的探讨甲状腺乳头状癌(PTC)组织中hMLH1基因启动子甲基化及其临床意义.方法采用甲基化特异性聚合酶链反应法检测152例PTC组织及癌旁正常组织中hMLH1基因启动子甲基化状态,并分析其与临床病理特征的关系.结果 152例PTC组织中,hMLH1基因启动子甲基化57例,其中完全甲基化33例,部分甲基化24例,甲基化率为37.5％.152例癌旁正常组织中,hMLH1基因启动子甲基化8例(均为部分甲基化),甲基化率为5.3％.PTC组织中的hMLH1基因启动子甲基化率明显高于癌旁正常组织(P＜0.05).hMLH1基因启动子甲基化与原发灶大小、原发灶数量、局部侵犯情况、T分期和颈部淋巴结转移情况有关(均P ＜0.05),而与患者性别、病理分期无关(均P＞0.05).结论 hMLH1基因启动子甲基化是PTC组织中较常见的分子事件,与PTC的进展关系密切.
목적 탐토갑상선유두상암(PTC)조직중hMLH1기인계동자갑기화급기림상의의.방법 채용갑기화특이성취합매련반응법검측152례PTC조직급암방정상조직중hMLH1기인계동자갑기화상태,병분석기여림상병리특정적관계.결과 152례PTC조직중,hMLH1기인계동자갑기화57례,기중완전갑기화33례,부분갑기화24례,갑기화솔위37.5％.152례암방정상조직중,hMLH1기인계동자갑기화8례(균위부분갑기화),갑기화솔위5.3％.PTC조직중적hMLH1기인계동자갑기화솔명현고우암방정상조직(P＜0.05).hMLH1기인계동자갑기화여원발조대소、원발조수량、국부침범정황、T분기화경부림파결전이정황유관(균P ＜0.05),이여환자성별、병리분기무관(균P＞0.05).결론 hMLH1기인계동자갑기화시PTC조직중교상견적분자사건,여PTC적진전관계밀절.
Objective To investigate the aberrant promoter methylation of hMLH1 gene promoter and its clinical significance in papillary thyroid cancer (PTC).Methods methylation of hMLH1 gene promoter in the cancer tissue and matched tumor-adjacent normal tissue of 152 PTC patients were detected by real-time methylation specific PCR (qMSP).The relationship between the methylation of hMLH1 gene promoter and clinicopathological features was analyzed.Results The methylation rate of hMLH1 gene promoter in cancer tissues was 37.5％ (57/152),of which 33 cases were totally methylated and 24 cases were partially methylated.The methylation rate of adjacent normal tissues was 5.3％ (8/152) (all were partially methylated).The methylation rate of PTC tissues was significantly higher than that in the tumoradjacent normal tissue (P ＜ 0.01).The promoter methylation of hMLH1 gene in PTC was significantly correlated with age,size and number of the primary lesion,local invasion,T stage and lymph node metastasis (P ＜0.05),but not correlated with gender and clinical stage (P ＞0.05).Conclusion Promoter methylation of hMLH1 gene is a common molecular event in PTC tissue,and it is significantly correlated with the progression of PTC.