CAJ | 학술논문

目的为取代后肢移植而构建1个与大鼠后肢成分相当、操作更加简便可靠的复合组织移植模型——股骨骨-肌皮瓣移植.方法将5只Lewis(RT11)大鼠双侧含腹股沟皮瓣和部分股骨的股骨骨-肌皮瓣,移植到10只同系Lewis大鼠的左侧腹股沟区,显微吻合供、受体间的营养血管即股动、静脉.术后通过大体和病理学检查观察各组移植物存活情况,检验模型的可行性.结果平均手术时间为(159.0 ±8.3) min,其中供体剥离时间(68.0±4.8) min,缺血时间(55.8±6.8) min.游离股骨骨-肌皮瓣美蓝灌注,显示股动、静脉可以保证股骨和皮瓣的血供.术后所有移植物长期存活,无一例坏死,皮瓣外观红润,有毛发生长.皮肤和股骨的HE染色也大致正常,进一步证实了移植物的活性.结论带血管蒂的股骨骨-肌皮瓣是一个简便可靠的复合组织移植模型,该模型的建立,将为异体复合组织移植的研究提供新的方法.
목적 위취대후지이식이구건1개여대서후지성분상당、조작경가간편가고적복합조직이식모형——고골골-기피판이식.방법 장5지Lewis(RT11)대서쌍측함복고구피판화부분고골적고골골-기피판,이식도10지동계Lewis대서적좌측복고구구,현미문합공、수체간적영양혈관즉고동、정맥.술후통과대체화병이학검사관찰각조이식물존활정황,검험모형적가행성.결과 평균수술시간위(159.0 ±8.3) min,기중공체박리시간(68.0±4.8) min,결혈시간(55.8±6.8) min.유리고골골-기피판미람관주,현시고동、정맥가이보증고골화피판적혈공.술후소유이식물장기존활,무일례배사,피판외관홍윤,유모발생장.피부화고골적HE염색야대치정상,진일보증실료이식물적활성.결론 대혈관체적고골골-기피판시일개간편가고적복합조직이식모형,해모형적건립,장위이체복합조직이식적연구제공신적방법.
Objective To reconstruct a simpler and reliable composite tissue transplantation model-the femur osteomyocutaneous flap for the replacement of hindlimb transplantation.Methods Ten femur osteomyocutaneous flaps from 5 Lewis rats were transplanted into 10 syngeneic recipients' inguinal re'on.Their nutrient vessels were anastomosed with recipients vessels.The graft of this model was consisted of the groin flap and partial femur.To verify the feasibility of this model,gross and histological appearance werestudied after transplantation to evaluate the viability of grafts.Results The operative time was (159.0 ±8.3) min with the harvesting time of (68.0 ±4.8) min and the ischemia time of (55.8 ± 6.8) min.The methylene blue injection showed rich blood supply of transplanted femur osteomyocutaneous flap.All the 10 flaps survived completely with pink skin color and hair regrowth.The histologic examination of the flaps also revealed the normal appearance of the viable skin and bone marrow.Conclusions The femur osteomyocutaneous flap is a simple and reliable model for composite tissue transplantation,and its establishment will provide a new tool for the study of composite tissue allografts.