中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2014年
4期
274-278
,共5页
徐广琪%牛静静%吕仁荣%周生儒%霍然
徐廣琪%牛靜靜%呂仁榮%週生儒%霍然
서엄기%우정정%려인영%주생유%곽연
婴幼儿血管瘤%β肾上腺素受体-2%血管内皮生长因子受体-2
嬰幼兒血管瘤%β腎上腺素受體-2%血管內皮生長因子受體-2
영유인혈관류%β신상선소수체-2%혈관내피생장인자수체-2
Infantile hemangioma%β-adrenergic receptor 2%Vascular endothelial growth factor-2
目的 检测不同时期婴幼儿血管瘤组织中β肾上腺素受体-2和血管内皮生长因子受体-2的表达,并探讨两者在血管瘤的病理演变过程中的意义.方法 按照Mulliken分类法将血管瘤分为三期:增生期(＜12个月)32例,消退期(13 ～ 60个月)17例和消退完成期(61个月至12岁)11例;另收集7例瘤旁正常皮肤作为对照.通过免疫组化法检测增生期、消退期、消退完成期婴幼儿血管瘤组织中β肾上腺素受体-2和血管内皮生长因子受体-2的表达情况,并利用计算机图像分析技术(Image Pro Plus 6.0软件)测量平均吸光度值.数据统计以均数±标准差表示,采用SPSS16.0软件对定量结果进行单因素方差分析.结果 β肾上腺素受体-2在增生期血管瘤呈强阳性表达、消退期呈阳性表达、消退完成期呈弱阳性表达,在各期平均吸光度值分别为0.064 751 2±0.012 747、0.031 601 7±0.006 848、0.011 869 8±0.039 349;血管内皮生长因子受体-2在血管瘤增生期呈强阳性表达、消退期呈阳性表达、消退完成期呈弱阳性表达,在各期平均吸光度值分别为0.068 940 9±0.029 274、0.028 445 5±0.006 396、0.011 184 1±0.004 198;各期之间差异有统计学意义(P ＜0.05);β肾上腺素受体-2与血管内皮细胞生长因子受体-2平均吸光度值的相关性分析具有统计学意义(P＜0.05).结论 β肾上腺素受体-2和血管内皮生长因子受体-2可能参与了血管瘤发生、发展及消退的病理过程.
目的 檢測不同時期嬰幼兒血管瘤組織中β腎上腺素受體-2和血管內皮生長因子受體-2的錶達,併探討兩者在血管瘤的病理縯變過程中的意義.方法 按照Mulliken分類法將血管瘤分為三期:增生期(＜12箇月)32例,消退期(13 ～ 60箇月)17例和消退完成期(61箇月至12歲)11例;另收集7例瘤徬正常皮膚作為對照.通過免疫組化法檢測增生期、消退期、消退完成期嬰幼兒血管瘤組織中β腎上腺素受體-2和血管內皮生長因子受體-2的錶達情況,併利用計算機圖像分析技術(Image Pro Plus 6.0軟件)測量平均吸光度值.數據統計以均數±標準差錶示,採用SPSS16.0軟件對定量結果進行單因素方差分析.結果 β腎上腺素受體-2在增生期血管瘤呈彊暘性錶達、消退期呈暘性錶達、消退完成期呈弱暘性錶達,在各期平均吸光度值分彆為0.064 751 2±0.012 747、0.031 601 7±0.006 848、0.011 869 8±0.039 349;血管內皮生長因子受體-2在血管瘤增生期呈彊暘性錶達、消退期呈暘性錶達、消退完成期呈弱暘性錶達,在各期平均吸光度值分彆為0.068 940 9±0.029 274、0.028 445 5±0.006 396、0.011 184 1±0.004 198;各期之間差異有統計學意義(P ＜0.05);β腎上腺素受體-2與血管內皮細胞生長因子受體-2平均吸光度值的相關性分析具有統計學意義(P＜0.05).結論 β腎上腺素受體-2和血管內皮生長因子受體-2可能參與瞭血管瘤髮生、髮展及消退的病理過程.
목적 검측불동시기영유인혈관류조직중β신상선소수체-2화혈관내피생장인자수체-2적표체,병탐토량자재혈관류적병리연변과정중적의의.방법 안조Mulliken분류법장혈관류분위삼기:증생기(＜12개월)32례,소퇴기(13 ～ 60개월)17례화소퇴완성기(61개월지12세)11례;령수집7례류방정상피부작위대조.통과면역조화법검측증생기、소퇴기、소퇴완성기영유인혈관류조직중β신상선소수체-2화혈관내피생장인자수체-2적표체정황,병이용계산궤도상분석기술(Image Pro Plus 6.0연건)측량평균흡광도치.수거통계이균수±표준차표시,채용SPSS16.0연건대정량결과진행단인소방차분석.결과 β신상선소수체-2재증생기혈관류정강양성표체、소퇴기정양성표체、소퇴완성기정약양성표체,재각기평균흡광도치분별위0.064 751 2±0.012 747、0.031 601 7±0.006 848、0.011 869 8±0.039 349;혈관내피생장인자수체-2재혈관류증생기정강양성표체、소퇴기정양성표체、소퇴완성기정약양성표체,재각기평균흡광도치분별위0.068 940 9±0.029 274、0.028 445 5±0.006 396、0.011 184 1±0.004 198;각기지간차이유통계학의의(P ＜0.05);β신상선소수체-2여혈관내피세포생장인자수체-2평균흡광도치적상관성분석구유통계학의의(P＜0.05).결론 β신상선소수체-2화혈관내피생장인자수체-2가능삼여료혈관류발생、발전급소퇴적병리과정.
Objective To investigate the significance of β-adrenergic receptor 2 (β2-AR) and vascular endothelial growth factor-2 (VEGFR-2) in the occurrence and development of infantile hemangioma through detecting the expression of β2-AR and VEGFR-2 in the different stages of infantile hemangiomas.Methods According to the Mulliken's classification standard,we classified the specimens as proliferating group (32 cases),involuting group (17 cases) and involuted group (11 cases).Normal skin tissue surrounding the hemangioma from 7 cases were chosen as control group.The expression of β2-AR and VEGFR-2 was detected by immunohistochemical technique in proliferating hemangioma,involuting hemangioma,involuted hemangioma.The mean optical density was measured by image analysis system (Image Pro Plus 6.0) and SPSS 16.0 software was applied for statistical analysis.Results The expression of β2-AR and VEGFR-2 was strongly positive in proliferating hemangioma,while positive in involuting hemangioma and weakly positive in the involuted stage.The mean optical density of each phase was 0.064 751 2 ±0.012 747,0.031 601 7 ±0.006 848,0.011 869 8 ±0.039 349 for β2-AR,and 0.068 940 9±0.029 274,0.028 445 5 ±0.006 396,0.011 184 1 ±0.004 198 for VEGFR-2.The differences between different stages had a statistically significance(P ＜ 0.05).Correlation analysis on the mean optical density between β2-AR and VEGFR-2 had a statistically significance (P ＜ 0.05).Conclusions β2-AR and VEGFR-2 may be involved in the occurrence and development of infantile hemangioma.
