中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2014年
4期
289-293
,共5页
周树萍%黄智龙%马继光%徐静静%蔡景龙%宗宪磊%都乐
週樹萍%黃智龍%馬繼光%徐靜靜%蔡景龍%宗憲磊%都樂
주수평%황지룡%마계광%서정정%채경룡%종헌뢰%도악
瘢痕,肥大性%表皮干细胞%伤口愈合
瘢痕,肥大性%錶皮榦細胞%傷口愈閤
반흔,비대성%표피간세포%상구유합
Hypertrophic scar%Epidermal stem cells%Wound healing
目的 探讨人成熟期增生性瘢痕来源的表皮干细胞(ESCs)修复裸鼠皮肤缺损的效果.方法 选择40只裸鼠,在每只裸鼠背部制造一直径1 cm的创面,随机分为实验组和对照组,每组20只.实验组在创面上给予人增生性瘢痕-ESCs+红霉素眼膏治疗,对照组创面只外用红霉素跟膏,其他处理一致.观察裸鼠背部创面愈合时间及14 d时创面组织的修复情况、Ⅰ和Ⅲ型胶原、表皮生长因子(EGF)、成纤维生长因子(FGF2)、转化生长因子(TGFβ1和TGFβ2)的表达情况.结果 创面愈合时间实验组为(20.8±0.84)d,对照组为(25.6±0.89) d;HE染色显示实验组的血管化数目为(11.60±0.55)支,对照组为(8.04±0.33)支;免疫组织化学检测显示实验组皮肤的Ⅰ、Ⅲ型胶原及EGF、FGF2、TGFβ1和TGFβ2分泌能力更强;这些指标在2组之间的差异均具有统计学意义(P＜0.05).结论 增生性瘢痕-ESCs能够促进创面的愈合,其机制可能是促进了创面组织血管化和生长因子的分泌.
目的 探討人成熟期增生性瘢痕來源的錶皮榦細胞(ESCs)脩複裸鼠皮膚缺損的效果.方法 選擇40隻裸鼠,在每隻裸鼠揹部製造一直徑1 cm的創麵,隨機分為實驗組和對照組,每組20隻.實驗組在創麵上給予人增生性瘢痕-ESCs+紅黴素眼膏治療,對照組創麵隻外用紅黴素跟膏,其他處理一緻.觀察裸鼠揹部創麵愈閤時間及14 d時創麵組織的脩複情況、Ⅰ和Ⅲ型膠原、錶皮生長因子(EGF)、成纖維生長因子(FGF2)、轉化生長因子(TGFβ1和TGFβ2)的錶達情況.結果 創麵愈閤時間實驗組為(20.8±0.84)d,對照組為(25.6±0.89) d;HE染色顯示實驗組的血管化數目為(11.60±0.55)支,對照組為(8.04±0.33)支;免疫組織化學檢測顯示實驗組皮膚的Ⅰ、Ⅲ型膠原及EGF、FGF2、TGFβ1和TGFβ2分泌能力更彊;這些指標在2組之間的差異均具有統計學意義(P＜0.05).結論 增生性瘢痕-ESCs能夠促進創麵的愈閤,其機製可能是促進瞭創麵組織血管化和生長因子的分泌.
목적 탐토인성숙기증생성반흔래원적표피간세포(ESCs)수복라서피부결손적효과.방법 선택40지라서,재매지라서배부제조일직경1 cm적창면,수궤분위실험조화대조조,매조20지.실험조재창면상급여인증생성반흔-ESCs+홍매소안고치료,대조조창면지외용홍매소근고,기타처리일치.관찰라서배부창면유합시간급14 d시창면조직적수복정황、Ⅰ화Ⅲ형효원、표피생장인자(EGF)、성섬유생장인자(FGF2)、전화생장인자(TGFβ1화TGFβ2)적표체정황.결과 창면유합시간실험조위(20.8±0.84)d,대조조위(25.6±0.89) d;HE염색현시실험조적혈관화수목위(11.60±0.55)지,대조조위(8.04±0.33)지;면역조직화학검측현시실험조피부적Ⅰ、Ⅲ형효원급EGF、FGF2、TGFβ1화TGFβ2분비능력경강;저사지표재2조지간적차이균구유통계학의의(P＜0.05).결론 증생성반흔-ESCs능구촉진창면적유합,기궤제가능시촉진료창면조직혈관화화생장인자적분비.
Objective To investigate the effect of epidermal stem cells from human hypertrophic scar (HS-ESCs)on the skin wound healing in nude mice.Methods 40 mice were randomly divided into two groups as experimental group(n =20) and control group(n =20).Wounds,1 cm in diameters,were made on every mouse back.The wounds were treated with HS-ESCs and erythromycin ointment in experimental group,or only with erythromycin ointment in control group.The wound healing was observed during the following 14 days.The expression of collagen-Ⅰ,collagen-Ⅲ,epidermal growth factor (EGF),fibroblast growth factor (FGF2),transforming growth factor (TGFβ1 and TGFβ2) were studied.Results The wound healing time in the experimental group was (20.8 ± 0.84) d,which was (25.6 ± 0.89) d in the control group.HE staining revealed that the extent of vascularization in the experimental group was 11.60 ± 0.55,while it was 8.04 ± 0.33 in the control group.Immunochemistry analysis showed the expression of collagen-Ⅰ,collagen-Ⅲ,EGF,FGF2,TGFβ1 and TGFβ2 in the experimental group were significantly higher,compared with those in control group (P ＜ 0.05).Conclusion HS-ESCs may promote wound healing through enhancement of the vascularization of the wound tissue and the expression of growth factors.
