CAJ | 학술논문

目的探索普萘洛尔治疗婴幼儿血管瘤的机制,以及其对血管瘤间充质干细胞增殖的影响.方法采用贴壁筛选法从增生期血管瘤中分离间充质干细胞,应用免疫荧光染色检测细胞表型.以人脐静脉内皮细胞做对照,在体外诱导2组细胞向脂肪细胞、成骨细胞分化,以鉴定血管瘤间充质干细胞的多向分化潜能.采用MTT法测定不同浓度的普萘洛尔于用药后0、24、48、72、96、120 h对血管瘤间充质干细胞增殖的影响.结果血管瘤间充质干细胞呈成纤维细胞样,表达Vimentin,大部分表达α-SMA及CD133,部分表达Glutl,不表达VEGF.血管瘤间充质干细胞可向脂肪细胞、成骨细胞分化.用药后24 h,15 mg/L(B组)的吸光度A值(0.596 0 ±0.023 6)较0 mg/L组(A组,0.552 7 ±0.011 4)大,且差异有统计学意义(P＜0.05),而用药后各时相点,30 mg/L(C组,除24 h外)、45 mg/L(D组)与A组比较,差异均有统计学意义(P＜0.05),即低浓度普萘洛尔对血管瘤间充质干细胞的增殖影响不甚明显,高浓度普萘洛尔可抑制血管瘤间充质干细胞生长.结论本研究中分离所得细胞为血管瘤间充质干细胞,高浓度的普萘洛尔可抑制血管瘤间充质干细胞生长.
목적 탐색보내락이치료영유인혈관류적궤제,이급기대혈관류간충질간세포증식적영향.방법 채용첩벽사선법종증생기혈관류중분리간충질간세포,응용면역형광염색검측세포표형.이인제정맥내피세포주대조,재체외유도2조세포향지방세포、성골세포분화,이감정혈관류간충질간세포적다향분화잠능.채용MTT법측정불동농도적보내락이우용약후0、24、48、72、96、120 h대혈관류간충질간세포증식적영향.결과 혈관류간충질간세포정성섬유세포양,표체Vimentin,대부분표체α-SMA급CD133,부분표체Glutl,불표체VEGF.혈관류간충질간세포가향지방세포、성골세포분화.용약후24 h,15 mg/L(B조)적흡광도A치(0.596 0 ±0.023 6)교0 mg/L조(A조,0.552 7 ±0.011 4)대,차차이유통계학의의(P＜0.05),이용약후각시상점,30 mg/L(C조,제24 h외)、45 mg/L(D조)여A조비교,차이균유통계학의의(P＜0.05),즉저농도보내락이대혈관류간충질간세포적증식영향불심명현,고농도보내락이가억제혈관류간충질간세포생장.결론 본연구중분리소득세포위혈관류간충질간세포,고농도적보내락이가억제혈관류간충질간세포생장.
Objective To explore the new mechanism of propranolol for treatment of hemangioma and the effects of propranolol on proliferation of hemangioma-derived mesenchymal stem cells (Hem-MSCs).Methods We isolated Hem-MSCs from hemangioma in the proliferating phase by their selective adhesion to plastic culture dishes.Immunofluorescence staining was used to examine the expression of marker antigens in Hem-MSCs.Human umbilical vein endothelial cells(HUVECs) were used as control.Induction of multi-lineage differentiation including osteogenesis and adipogeueis was performed with appropriate medium to identify the multi-lineage differentiation potential.MTT cell counting was used to observe the effects of different concentrations of propranolol on proliferation of Hem-MSCs.Results Hem-MSCs were fibroblast-like morphology.All of them expressed vimentin,most expressed α-SMA、CD133,some expressed Glutl,and none of them expressed VEGF.Osteogenic,adipogenic differentiations of Hem-MSCs were induced successfully.Effects of low concentration of propranolol on proliferation of Hem-MSCs were not obvious,while high concentration of propranolol can inhibit the proliferation of Hem-MSCs.Conclusions The cells we isolated from hemangioma are Hem-MSCs.High concentration of propranolol can inhibit the proliferation of Hem-MSCs.