CAJ | 학술논문

目的探讨皮肤滚针联合聚桂醇对毛细血管畸形的作用特点.方法 1.5 ～2.0 kg雄性莱航鸡24只,随机分为3组,每组8只.A组:皮肤滚针+聚桂醇;B组:皮肤滚针+生理盐水;C组:正常鸡冠.分别在皮肤滚针联合聚桂醇干预鸡冠后的第7、14、21、28天切取实验区域的鸡冠组织,制作病理切片后行HE染色、免疫组化染色和维多利亚蓝-丽春红染色,分别计算毛细血管数目和Ⅰ型、Ⅲ型胶原的纤维面积、弹力纤维面积.结果肉眼观察:鸡冠组织的颜色随时间的推移而变浅;HE染色显示:A组毛细血管数目随时间的延长明显减少;Ⅰ型胶原纤维及Ⅲ型胶原纤维面积百分比计算结果:B组与A、C组对比,差异有统计学意义(P＜0.05);特殊染色结果显示:B组弹力纤维的表达有增生趋势.结论动物实验显示,皮肤滚针联合聚桂醇干预鸡冠组织后可以有效地破坏、栓塞毛细血管网,且不导致局部组织的纤维化;单独应用皮肤滚针可以刺激真皮层弹力纤维轻度增生,可以延缓皮肤衰老的自然过程.
목적 탐토피부곤침연합취계순대모세혈관기형적작용특점.방법 1.5 ～2.0 kg웅성래항계24지,수궤분위3조,매조8지.A조:피부곤침+취계순;B조:피부곤침+생리염수;C조:정상계관.분별재피부곤침연합취계순간예계관후적제7、14、21、28천절취실험구역적계관조직,제작병리절편후행HE염색、면역조화염색화유다리아람-려춘홍염색,분별계산모세혈관수목화Ⅰ형、Ⅲ형효원적섬유면적、탄력섬유면적.결과 육안관찰:계관조직적안색수시간적추이이변천;HE염색현시:A조모세혈관수목수시간적연장명현감소;Ⅰ형효원섬유급Ⅲ형효원섬유면적백분비계산결과:B조여A、C조대비,차이유통계학의의(P＜0.05);특수염색결과현시:B조탄력섬유적표체유증생추세.결론 동물실험현시,피부곤침연합취계순간예계관조직후가이유효지파배、전새모세혈관망,차불도치국부조직적섬유화;단독응용피부곤침가이자격진피층탄력섬유경도증생,가이연완피부쇠로적자연과정.
Objective To explore the effect of microneedle combined with Lauromacrogol on skin capillary network.Methods 24 male Leghone (1.5-2.0 kg in weight) were randomly divided into three groups as group A (microneedle combined with Lauromacrogol),B (microneedle combined with physiological saline),and C(control).The cockscombs were treated.The specimens were taken on the 7th,14th,21th,and 28th day postoperatively.HE staining,immunohistochemical staining and special staining were performed for study of the number of capillary and collagen Ⅰ /Ⅱ,as well as elastic fibers.Results The color of cockscombs in group A became lightening after treatment.The number of capillary decreased as showing by HE staining.The collagen Ⅰ and Ⅲ in group B was significantly different from that in group A and C (P ＜ 0.05).Special staining showed proliferation of elastic fibers in group B.Conclusions It indicates that microneedle combined with Lauromacrogol could effectively reduce the capillary in cockscomb without any tissue fibrosis.Microneedle can stimulate the proliferation of elastic fiber,so as to improve the skin ageing process.