肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2010年
6期
388-390,395
,共4页
卵巢肿瘤%细胞%凋亡诱导因子%苏木
卵巢腫瘤%細胞%凋亡誘導因子%囌木
란소종류%세포%조망유도인자%소목
Ovarian neoplasms%Ceils%Apoptosis inducing factor%Lignum sappan
目的 研究苏木水提物对人类卵巢癌细胞SKOV3的诱导凋亡机制.方法 采用免疫细胞化学法测定不同质量浓度苏木水提物作用后SKOV3细胞中Caspase-3、Caspase-9及Survivin的表达.结果 随着苏木水提物质量浓度的增加Caspase-3、Caspase-9阳性表达率增加,灰度值降低;Survivin 阳性表达率降低,灰度值升高.苏木水提物质量物浓度为0、25、50、100、200 mg/L时,Caspase-3的阳性表达率依次为5.29%、12.61%、24.60%、100.00%、100.00%;Caspase-9的阳性表达率依次为12.32%、29.17%、57.42%、100.00%、100.00%;Survivin的阳性表达率依次为81.62%、60.53%、36.94%、55.69%、36.92%.结论 苏木水提物通过增加Caspase-3、Caspase-9的表达及抑制Survivin的表达而诱导SKOV3细胞凋亡.
目的 研究囌木水提物對人類卵巢癌細胞SKOV3的誘導凋亡機製.方法 採用免疫細胞化學法測定不同質量濃度囌木水提物作用後SKOV3細胞中Caspase-3、Caspase-9及Survivin的錶達.結果 隨著囌木水提物質量濃度的增加Caspase-3、Caspase-9暘性錶達率增加,灰度值降低;Survivin 暘性錶達率降低,灰度值升高.囌木水提物質量物濃度為0、25、50、100、200 mg/L時,Caspase-3的暘性錶達率依次為5.29%、12.61%、24.60%、100.00%、100.00%;Caspase-9的暘性錶達率依次為12.32%、29.17%、57.42%、100.00%、100.00%;Survivin的暘性錶達率依次為81.62%、60.53%、36.94%、55.69%、36.92%.結論 囌木水提物通過增加Caspase-3、Caspase-9的錶達及抑製Survivin的錶達而誘導SKOV3細胞凋亡.
목적 연구소목수제물대인류란소암세포SKOV3적유도조망궤제.방법 채용면역세포화학법측정불동질량농도소목수제물작용후SKOV3세포중Caspase-3、Caspase-9급Survivin적표체.결과 수착소목수제물질량농도적증가Caspase-3、Caspase-9양성표체솔증가,회도치강저;Survivin 양성표체솔강저,회도치승고.소목수제물질량물농도위0、25、50、100、200 mg/L시,Caspase-3적양성표체솔의차위5.29%、12.61%、24.60%、100.00%、100.00%;Caspase-9적양성표체솔의차위12.32%、29.17%、57.42%、100.00%、100.00%;Survivin적양성표체솔의차위81.62%、60.53%、36.94%、55.69%、36.92%.결론 소목수제물통과증가Caspase-3、Caspase-9적표체급억제Survivin적표체이유도SKOV3세포조망.
Objective To investigate the mechanism of oaesalpinia sappan aqueous extract's apoptosis-inducing on human ovarian cancer cell line SKOV3.Methods With different concentration of eaesalpinia sappan aqueous extract acting on cells,immunocytochemistry was used to detect the expression of Caspase-3,9 and survivin assay in ovarian cancer cell line SKOV3.Results The positive rate of Caspase-3,9 were increased with the increasing concentration of caesalpinia sappan aqueous extract,while the gray scale was decreased.The positive rates of surviving were decreased with the increasing concentration of caesalpinia sappan aqueous extract,while the gray scale were increased.At the concentration of 0,25 μg/ml,50μg,ml,100μs/ml,200μg/ml,the positive rates of Caspase-3 were 5.29%,12.61%,24.60%,100.00%,100.00%,respectively;the positive rates of Caspase-9 were 12.32%,29.17%,57.42%,100.00%,100.00%,respectively;the positive rates of survivin were 8 1.62%,60.53%,36.94%,55.69%,36.92%,respectively.Conclusion Caesalpinia sappan aqueous extract can obviously induce apoptosis of human ovarian cancer ceil line SKOV3 by increasing expression of Caspase-3,9 and decreasing expression of survivin.
