肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2013年
1期
5-7
,共3页
张小龙%张涛%刘焕义%赵振国
張小龍%張濤%劉煥義%趙振國
장소룡%장도%류환의%조진국
水疱口炎病毒%A549细胞系%RNA干扰%Livin
水皰口炎病毒%A549細胞繫%RNA榦擾%Livin
수포구염병독%A549세포계%RNA간우%Livin
Vesicular stomatitis virus%A549 cells%RNA interference%Livin
目的 探讨Livin表达影响水疱口炎病毒(VSV)对肺癌A549细胞的促凋亡效应.方法 利用RNA干扰技术下调Livin在肿瘤细胞中的表达,通过Tunel染色观察VSV对肺癌A549细胞的凋亡效应,Western blot法检测Livin蛋白的表达,荧光定量法检测Caspase-3的活性.结果 VSV(感染复数=0.1 pfu/细胞)对A549细胞有促凋亡作用；抑制Livin的表达增强了VSV对A549细胞的促凋亡作用,增强了Caspase-3的活性.结论 Livin表达沉默后可能通过影响Caspase-3活性而增强了VSV对A549细胞的促凋亡效应.
目的 探討Livin錶達影響水皰口炎病毒(VSV)對肺癌A549細胞的促凋亡效應.方法 利用RNA榦擾技術下調Livin在腫瘤細胞中的錶達,通過Tunel染色觀察VSV對肺癌A549細胞的凋亡效應,Western blot法檢測Livin蛋白的錶達,熒光定量法檢測Caspase-3的活性.結果 VSV(感染複數=0.1 pfu/細胞)對A549細胞有促凋亡作用；抑製Livin的錶達增彊瞭VSV對A549細胞的促凋亡作用,增彊瞭Caspase-3的活性.結論 Livin錶達沉默後可能通過影響Caspase-3活性而增彊瞭VSV對A549細胞的促凋亡效應.
목적 탐토Livin표체영향수포구염병독(VSV)대폐암A549세포적촉조망효응.방법 이용RNA간우기술하조Livin재종류세포중적표체,통과Tunel염색관찰VSV대폐암A549세포적조망효응,Western blot법검측Livin단백적표체,형광정량법검측Caspase-3적활성.결과 VSV(감염복수=0.1 pfu/세포)대A549세포유촉조망작용；억제Livin적표체증강료VSV대A549세포적촉조망작용,증강료Caspase-3적활성.결론 Livin표체침묵후가능통과영향Caspase-3활성이증강료VSV대A549세포적촉조망효응.
Objective To investigate the effect of Livin expression on VSV-induced apoptsis of A549 cells.Methods The expression of Livin of A549 cells was inhibited by RNA interference.VSV-induced apoptosis of A549 cells was observed by Tunel assay.Protein Level of livin was detected by Western blot.Caspase-3 activity was detected by the fluorescence-based quantitative method.Results Livin downregulation VSV-induced apoptosis of A549 cells.Inhibited the expression of Livin of A549 cells had increased Caspase-3 activity.Conclusion The effect of Livin on VSV-induced apoptotic of A549 cells could be increased by RNA interference.
