肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2013年
2期
73-76
,共4页
王少彬%黄万钟%钟瑜%李晓洁%陈俊辉
王少彬%黃萬鐘%鐘瑜%李曉潔%陳俊輝
왕소빈%황만종%종유%리효길%진준휘
冬凌草甲素%人类食管癌SHEEC细胞%基因芯片%荧光定量聚合酶链反应
鼕凌草甲素%人類食管癌SHEEC細胞%基因芯片%熒光定量聚閤酶鏈反應
동릉초갑소%인류식관암SHEEC세포%기인심편%형광정량취합매련반응
Oridonin%Human esophageal carcinoma cell SHEEC%Gene chip%Fluorecent quantitative polymerase chain reaction
目的 检测冬凌草甲素(ORI)对人类食管癌SHEEC细胞基因表达的影响并筛选与细胞凋亡密切相关的靶基因.方法 应用基因芯片技术检测32μg/ml ORI作用SHEEC细胞1h及8h前后的基因表达差异,分析筛选差异表达基因,并用荧光定量PCR进行验证.结果 32 μg/ml ORI作用SHEEC细胞1h及8h后,表达上调≥2倍及表达下调≥2倍的基因共1011个,其中,1h有280个,8h有731个.在1011个差异表达基因中,最后筛选出17个重复检测表达上调或表达下调幅度大且与细胞凋亡、信号转导和转录调控相关的基因.荧光定量PCR对17个差异表达基因进行验证,其中12个基因具有统计学意义.结论 在12个有统计学意义的差异表达基因中,可能有ORI经线粒体途径诱导肿瘤细胞凋亡的靶基因.
目的 檢測鼕凌草甲素(ORI)對人類食管癌SHEEC細胞基因錶達的影響併篩選與細胞凋亡密切相關的靶基因.方法 應用基因芯片技術檢測32μg/ml ORI作用SHEEC細胞1h及8h前後的基因錶達差異,分析篩選差異錶達基因,併用熒光定量PCR進行驗證.結果 32 μg/ml ORI作用SHEEC細胞1h及8h後,錶達上調≥2倍及錶達下調≥2倍的基因共1011箇,其中,1h有280箇,8h有731箇.在1011箇差異錶達基因中,最後篩選齣17箇重複檢測錶達上調或錶達下調幅度大且與細胞凋亡、信號轉導和轉錄調控相關的基因.熒光定量PCR對17箇差異錶達基因進行驗證,其中12箇基因具有統計學意義.結論 在12箇有統計學意義的差異錶達基因中,可能有ORI經線粒體途徑誘導腫瘤細胞凋亡的靶基因.
목적 검측동릉초갑소(ORI)대인류식관암SHEEC세포기인표체적영향병사선여세포조망밀절상관적파기인.방법 응용기인심편기술검측32μg/ml ORI작용SHEEC세포1h급8h전후적기인표체차이,분석사선차이표체기인,병용형광정량PCR진행험증.결과 32 μg/ml ORI작용SHEEC세포1h급8h후,표체상조≥2배급표체하조≥2배적기인공1011개,기중,1h유280개,8h유731개.재1011개차이표체기인중,최후사선출17개중복검측표체상조혹표체하조폭도대차여세포조망、신호전도화전록조공상관적기인.형광정량PCR대17개차이표체기인진행험증,기중12개기인구유통계학의의.결론 재12개유통계학의의적차이표체기인중,가능유ORI경선립체도경유도종류세포조망적파기인.
Objective To detect the effect of oridonin(ORI)on the gene expression of human esophageal carcinoma cell SHEEC and to screen the tumor cell apoptosis target genes.Methods The gene expression of human esophageal carcinoma cell SHEEC without and with ORI induction for 1 hours and 8 hours were detected with microarray technique,respectively.The differentially expressed genes were identified and verified with fluorecent quantitative PCR.Results A total of 1011 genes showed up or down regulation more than twice after ORI induction(including 280 genes after 1 hour and 731 genes after 8 hours induction respectively).In these genes,17 genes with the top extent of up or down regulation were identified,which were involved in the cell signal transduction,transcription regulation,and cell apoptosis.These 17 differentially expressed genes were verified with real-time PCR,and 12 genes were statistically significant.Conclusion In the 12 differentially expressed genes with statistically significance,there may have tumor cell apoptosis target genes induced by ORI through mitochondrion route.
