肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2013年
2期
100-103
,共4页
丁鸭锁%钱硕%季卫阳%王汉东%鲁晓杰
丁鴨鎖%錢碩%季衛暘%王漢東%魯曉傑
정압쇄%전석%계위양%왕한동%로효걸
脑膜瘤%瘤周水肿%血管内皮生长因子类
腦膜瘤%瘤週水腫%血管內皮生長因子類
뇌막류%류주수종%혈관내피생장인자류
Meningiomas%Peritumoral edema%Vascular endothelial growth factors
目的 研究血管内皮生长因子(VEGF)在脑膜瘤瘤周水肿形成中的作用.方法 对经手术证实的幕上脑膜瘤37例患者共40个脑膜瘤进行研究,通过免疫组织化学法及Western blot检测肿瘤组织和瘤周脑组织中VEGF的蛋白表达,通过反转录PCR法检测VEGF的mRNA表达情况,以与β-actin的比值为其相对表达量；通过水肿指数(EI)评估术前瘤周水肿的严重程度,并分析其与VEGF表达的相关性.结果 在VEGF阳性病例中,VEGF蛋白水平在肿瘤组织、瘤周水肿脑组织和正常脑组织之间呈梯度降低(相对表达量分别为0.38±0.08、0.20±0.03、0.04±0.02).在肿瘤组织中,VEGF的蛋白水平、RNA水平和EI呈正相关(r=0.892、0.875,均P＜0.05)；在瘤周脑组织中,仅VEGF的蛋白水平和EI呈正相关(r=0.912,P＜0.05),而RNA则几乎未表达(0.06±0.02).结论 VEGF在脑膜瘤瘤周水肿的形成过程中可能起着重要作用；VEGF可能是由肿瘤组织产生的,并扩散到瘤周脑组织中形成水肿.
目的 研究血管內皮生長因子(VEGF)在腦膜瘤瘤週水腫形成中的作用.方法 對經手術證實的幕上腦膜瘤37例患者共40箇腦膜瘤進行研究,通過免疫組織化學法及Western blot檢測腫瘤組織和瘤週腦組織中VEGF的蛋白錶達,通過反轉錄PCR法檢測VEGF的mRNA錶達情況,以與β-actin的比值為其相對錶達量；通過水腫指數(EI)評估術前瘤週水腫的嚴重程度,併分析其與VEGF錶達的相關性.結果 在VEGF暘性病例中,VEGF蛋白水平在腫瘤組織、瘤週水腫腦組織和正常腦組織之間呈梯度降低(相對錶達量分彆為0.38±0.08、0.20±0.03、0.04±0.02).在腫瘤組織中,VEGF的蛋白水平、RNA水平和EI呈正相關(r=0.892、0.875,均P＜0.05)；在瘤週腦組織中,僅VEGF的蛋白水平和EI呈正相關(r=0.912,P＜0.05),而RNA則幾乎未錶達(0.06±0.02).結論 VEGF在腦膜瘤瘤週水腫的形成過程中可能起著重要作用；VEGF可能是由腫瘤組織產生的,併擴散到瘤週腦組織中形成水腫.
목적 연구혈관내피생장인자(VEGF)재뇌막류류주수종형성중적작용.방법 대경수술증실적막상뇌막류37례환자공40개뇌막류진행연구,통과면역조직화학법급Western blot검측종류조직화류주뇌조직중VEGF적단백표체,통과반전록PCR법검측VEGF적mRNA표체정황,이여β-actin적비치위기상대표체량；통과수종지수(EI)평고술전류주수종적엄중정도,병분석기여VEGF표체적상관성.결과 재VEGF양성병례중,VEGF단백수평재종류조직、류주수종뇌조직화정상뇌조직지간정제도강저(상대표체량분별위0.38±0.08、0.20±0.03、0.04±0.02).재종류조직중,VEGF적단백수평、RNA수평화EI정정상관(r=0.892、0.875,균P＜0.05)；재류주뇌조직중,부VEGF적단백수평화EI정정상관(r=0.912,P＜0.05),이RNA칙궤호미표체(0.06±0.02).결론 VEGF재뇌막류류주수종적형성과정중가능기착중요작용；VEGF가능시유종류조직산생적,병확산도류주뇌조직중형성수종.
Objective To explore the formation mechanism of peritumoral brain edema(PTBE)by vascular endothelial growth factor(VEGF).Methods 40 biopsies were obtained from 37 patients.Inmunohistochemical staining and Western blot were performed to detect the expression of VEGF protein.Reverse-transcriptase polymerase chain reaction(RT-PCR)was used to analyze the presence and quantity of VEGF mRNA.The extent of PTBE was estimated as an edema index(EI)based on preoperative magnetic resonance imaging.Results In VEGF-positive cases,a decreasing gradient of VEGF protein expression was observed with increasing distance from tumors(0.38±0.08,0.20±0.03,0.04±0.02).In meningiomas,the protein level and the mRNA level were congruent and the expression of both protein and mRNA had a significant correlation with EI(protein: r =0.892,RNA: r =0.875,P ＜ 0.05).However,in peritumoral areas,protein level were not consistent with the mRNA level.Protein results showed high correlation with EI(r =0.912,P ＜ 0.05),but mRNA almost was almost undetectable(0.06±0.02).Conclusion VEGF is impartant on PTBE.It is concluded that VEGF macromolecules are secreted by tumor tissue and enter peritumoral normal brain tissue to induce edemagenesis in meningiomas.
