CAJ | 학술논문

目的分析人类端粒酶反转录酶(hTERT)及基质金属蛋白酶-7(MMP-7)mRNA在贲门癌患者外周血中的表达.方法采用实时荧光定量反转录-聚合酶链反应(RT-PCR)法检测157例贲门癌患者术前血液标本中MMP-7 mRNA和hTERT mRNA表达,对其表达与临床病理参数的关系进行比较.对无转移的81例患者术后6、12个月进行随访,根据患者临床症状、体征相应检查,判断复发转移.并将MMP-7 mRNA和hTERT mRNA表达阳性组与阴性组复发转移情况进行比较.结果 157例贲门癌患者中,hTERT阳性表达73例(46.0％)；MMP-7阳性表达61例(38.8％).hTERT、MMP-7表达与性别(x2值为2.597、0.199)、年龄(x2值为4.314、0.073)等因素无相关性(均P＞0.05),但与TNM分期(x2值为10.624、14.530)、有无远处转移(x2值为7.294、12.824)及组织的分化程度(x2值为12.003、6.482)之间存在相关性(均P＜0.05).术后随访6、12个月肿瘤转移率比较差异有统计学意义(P＜0.05).血行微转移阳性患者术后12个月肿瘤转移概率是阴性患者的6.12倍(OR=6.120).结论贲门癌患者外周血hTERT及MMP-7mRNA的异常表达与贲门癌的发展和转移有关,血液微转移检测对贲门癌早期发现及预后具有重要意义,值得进一步研究.
목적 분석인류단립매반전록매(hTERT)급기질금속단백매-7(MMP-7)mRNA재분문암환자외주혈중적표체.방법 채용실시형광정량반전록-취합매련반응(RT-PCR)법검측157례분문암환자술전혈액표본중MMP-7 mRNA화hTERT mRNA표체,대기표체여림상병리삼수적관계진행비교.대무전이적81례환자술후6、12개월진행수방,근거환자림상증상、체정상응검사,판단복발전이.병장MMP-7 mRNA화hTERT mRNA표체양성조여음성조복발전이정황진행비교.결과 157례분문암환자중,hTERT양성표체73례(46.0％)；MMP-7양성표체61례(38.8％).hTERT、MMP-7표체여성별(x2치위2.597、0.199)、년령(x2치위4.314、0.073)등인소무상관성(균P＞0.05),단여TNM분기(x2치위10.624、14.530)、유무원처전이(x2치위7.294、12.824)급조직적분화정도(x2치위12.003、6.482)지간존재상관성(균P＜0.05).술후수방6、12개월종류전이솔비교차이유통계학의의(P＜0.05).혈행미전이양성환자술후12개월종류전이개솔시음성환자적6.12배(OR=6.120).결론 분문암환자외주혈hTERT급MMP-7mRNA적이상표체여분문암적발전화전이유관,혈액미전이검측대분문암조기발현급예후구유중요의의,치득진일보연구.
Objective To detect the expressions of human telomerase tranacriptase (hTERT) and MMP-7 mRNA in the peripheral blood of patients with cardia cancer.Methods Total RNA was extracted with TRIzol and was transcribed reversely into cDNA.RT-PCR was used to detect mRNA expression of MMP-7 and hTERT in the peripheral blood of 157 patients with cardia cancer.81 patients without metastasis were followed up for 12 months.Results Of 157 patients,73 cases (46.0 ％) and 61 cases (38.8 ％) had the positive expressions of hTERT,MMP-7,respectively.There were significant correlations between the blood micro-metastases MMP-7 and hTERT expressions and TNM staging (x2 =10.624,14.530),tumor metastasis (x2 =7.294,12.824),and differentiation degree (x2 =12.003,6.482),but no significant correlations were seen in sex (x2 =2.597,0.199) and age (x2 =4.314,0.073).There was statistically significant in the occurrence rate of tumor metastasis between 6-month and 12-month follow-up.The occurrence rate of metastasis in patients with micro-metastases was 6.12-fold compared with patients without micro-metastases after 12-month follow-up.Conclusion Detection of MMP-7 and hTERT expressions can be used as reliable markers to predict peripheral blood micro-metastasis in patients with cardia carcinoma.