肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2013年
3期
169-172
,共4页
兰涛%苑建磊%王桂华%王钊
蘭濤%苑建磊%王桂華%王釗
란도%원건뢰%왕계화%왕쇠
癌,肝细胞%血管内皮生长因子%免疫组织化学%生存率
癌,肝細胞%血管內皮生長因子%免疫組織化學%生存率
암,간세포%혈관내피생장인자%면역조직화학%생존솔
Carcinoma,hepatocellular%Vascular endothelial growth factors%Immunohistochemistry%Survival rate
目的 检测Bag-1、血管内皮生长因子(VEGF)分别在肝细胞癌(简称:肝癌)组织、癌旁肝组织及正常肝组织中的表达,探讨它们与肝癌及其预后的关系.方法 采用免疫组织化学SP法检测Bag-1和VEGF在60例肝癌患者癌标本、30例癌旁肝组织、14例正常肝组织标本中的表达,并对其临床病理特点进行分析.结果 Bag-1、VEGF的表达率在肝癌组织中最高,分别为80.0％(48/60)及73.3％(44/60),并且与癌旁肝组织[46.7％(14/30)、43.3％(13/30)]、正常肝组织[28.6％(4/14)、21.4％(3/14)]比较,差异有统计学意义(P＜0.05).Bag-1、VEGF的表达与肝癌的分期、有无淋巴结转移、有无癌栓形成、有无包膜形成有相关性(P＜0.05),而与患者性别、年龄、肿瘤分级、肿瘤大小及甲胎蛋白(AFP)水平等因素无相关性(P＞0.05).Bag-1和VEGF在肝癌标本中同时阳性40例,同时阴性8例,二者在肝癌组织中的表达呈正相关(P＜0.05).Bag-1、VEGF阳性表达组患者1、2年生存率低于阴性组患者,阳性组患者的生存时间明显低于阴性组患者(P＜0.05).结论 Bag-1、VEGF与肝癌生物学特性密切相关,和肝癌患者预后密切相关.
目的 檢測Bag-1、血管內皮生長因子(VEGF)分彆在肝細胞癌(簡稱:肝癌)組織、癌徬肝組織及正常肝組織中的錶達,探討它們與肝癌及其預後的關繫.方法 採用免疫組織化學SP法檢測Bag-1和VEGF在60例肝癌患者癌標本、30例癌徬肝組織、14例正常肝組織標本中的錶達,併對其臨床病理特點進行分析.結果 Bag-1、VEGF的錶達率在肝癌組織中最高,分彆為80.0％(48/60)及73.3％(44/60),併且與癌徬肝組織[46.7％(14/30)、43.3％(13/30)]、正常肝組織[28.6％(4/14)、21.4％(3/14)]比較,差異有統計學意義(P＜0.05).Bag-1、VEGF的錶達與肝癌的分期、有無淋巴結轉移、有無癌栓形成、有無包膜形成有相關性(P＜0.05),而與患者性彆、年齡、腫瘤分級、腫瘤大小及甲胎蛋白(AFP)水平等因素無相關性(P＞0.05).Bag-1和VEGF在肝癌標本中同時暘性40例,同時陰性8例,二者在肝癌組織中的錶達呈正相關(P＜0.05).Bag-1、VEGF暘性錶達組患者1、2年生存率低于陰性組患者,暘性組患者的生存時間明顯低于陰性組患者(P＜0.05).結論 Bag-1、VEGF與肝癌生物學特性密切相關,和肝癌患者預後密切相關.
목적 검측Bag-1、혈관내피생장인자(VEGF)분별재간세포암(간칭:간암)조직、암방간조직급정상간조직중적표체,탐토타문여간암급기예후적관계.방법 채용면역조직화학SP법검측Bag-1화VEGF재60례간암환자암표본、30례암방간조직、14례정상간조직표본중적표체,병대기림상병리특점진행분석.결과 Bag-1、VEGF적표체솔재간암조직중최고,분별위80.0％(48/60)급73.3％(44/60),병차여암방간조직[46.7％(14/30)、43.3％(13/30)]、정상간조직[28.6％(4/14)、21.4％(3/14)]비교,차이유통계학의의(P＜0.05).Bag-1、VEGF적표체여간암적분기、유무림파결전이、유무암전형성、유무포막형성유상관성(P＜0.05),이여환자성별、년령、종류분급、종류대소급갑태단백(AFP)수평등인소무상관성(P＞0.05).Bag-1화VEGF재간암표본중동시양성40례,동시음성8례,이자재간암조직중적표체정정상관(P＜0.05).Bag-1、VEGF양성표체조환자1、2년생존솔저우음성조환자,양성조환자적생존시간명현저우음성조환자(P＜0.05).결론 Bag-1、VEGF여간암생물학특성밀절상관,화간암환자예후밀절상관.
Objective To detect the Bag-1 and VEGF expressions in HCC tissues,cancerous liver tissues and normal liver tissues,and to explore their relationship with prognosis.Methods Immunohistochemical staining (SP method) was used to detect Bag-1 and VEGF in 60 cases of hepatocellular carcinoma specimens,next to the 30 cases of hepatocellular carcinoma in liver tissue,and 14 cases of normal liver tissue specimens,and their clinicopathological features were analyzed.Results Bag-l,VEGF expression rates were highest in HCC tissues [80.0 ％ (48/60),73.3 ％ (44/60)] and with adjacent tissues [46.7 ％ (14/30),43.3 ％ (13/30)],normal liver tissue [28.6 ％ (4/14),21.4 ％ (3/14)] expression rates had differences (P ＜ 0.05).Bag-1,VEGF expression had relationship with liver cancer staging,lymph node metastasis,tumor thrombosis formation,the correlation between the presence or absence of capsule formation (P ＜ 0.05),while had no relationship with the patient gender,age,tumor grade,tumor size,and alpha-fetoprotein (AFP) and other factors correlation (P ＞ 0.05).Bag-1 and VEGF expression in HCC specimens positive 40 cases,while negative eight cases,both in HCC tissues expression was positively correlated (P ＜ 0.05).Bag-1,VEGF positive expression in patients 1-year,2-year survival rates were less than negative patients,the survival time of patients with positive group was significantly lower than the negative patients (P ＜ 0.05).Conclusion Bag-1 and VEGF expressions are closely related with biological characteristics of liver cancer,and which is closely related to the prognosis of patients with hepatocellular.
