肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2013年
3期
175-177
,共3页
结直肠肿瘤%Gli1%β-catenin
結直腸腫瘤%Gli1%β-catenin
결직장종류%Gli1%β-catenin
Colorectal neoplasms%Glil%β-catenin
目的 探讨Hedgehog信号通路、Wnt信号通路关键组成成员Gli1和β-catenin在大肠癌组织及肠癌细胞系中的表达协同情况,以及两种蛋白之间的相互作用关系.方法 采用荧光定量反转录聚合酶链反应(RT-PCR)结合Western blot方法检测Gli1、β-catenin在4例大肠癌组织及肠癌HCT116、HT-29、DLDl、SW620细胞系中表达情况,应用免疫共沉淀技术检测二者之间是否存在相互作用.结果 与癌旁正常组织相比,Gli1和β-catenin在大肠癌组织中均高表达[平均增长倍数分别为42.69和72.11(P＜ 0.05)].与HCT116相比,Gli1与β-catenin在HT-29、DLDI及SW620 3株大肠癌病变程度高的细胞中表达均升高[增长倍数分别为52.54、17.23、5.54及.5.30、6.34、2.78(均P＜0.05)],二者的表达具有协同性.免疫共沉淀结果显示Gli1与β-catenin两蛋白之间存在相互作用.结论 Gli1和β-catenin在大肠癌中协同表达,蛋白之间存在相互作用,提示Gli1、β-catenin共同参与肠癌发生、发展调控,可能是Hedgehog、Wnt信号通路交互作用的一条途径,对大肠癌的预防治疗可能有提示作用.
目的 探討Hedgehog信號通路、Wnt信號通路關鍵組成成員Gli1和β-catenin在大腸癌組織及腸癌細胞繫中的錶達協同情況,以及兩種蛋白之間的相互作用關繫.方法 採用熒光定量反轉錄聚閤酶鏈反應(RT-PCR)結閤Western blot方法檢測Gli1、β-catenin在4例大腸癌組織及腸癌HCT116、HT-29、DLDl、SW620細胞繫中錶達情況,應用免疫共沉澱技術檢測二者之間是否存在相互作用.結果 與癌徬正常組織相比,Gli1和β-catenin在大腸癌組織中均高錶達[平均增長倍數分彆為42.69和72.11(P＜ 0.05)].與HCT116相比,Gli1與β-catenin在HT-29、DLDI及SW620 3株大腸癌病變程度高的細胞中錶達均升高[增長倍數分彆為52.54、17.23、5.54及.5.30、6.34、2.78(均P＜0.05)],二者的錶達具有協同性.免疫共沉澱結果顯示Gli1與β-catenin兩蛋白之間存在相互作用.結論 Gli1和β-catenin在大腸癌中協同錶達,蛋白之間存在相互作用,提示Gli1、β-catenin共同參與腸癌髮生、髮展調控,可能是Hedgehog、Wnt信號通路交互作用的一條途徑,對大腸癌的預防治療可能有提示作用.
목적 탐토Hedgehog신호통로、Wnt신호통로관건조성성원Gli1화β-catenin재대장암조직급장암세포계중적표체협동정황,이급량충단백지간적상호작용관계.방법 채용형광정량반전록취합매련반응(RT-PCR)결합Western blot방법검측Gli1、β-catenin재4례대장암조직급장암HCT116、HT-29、DLDl、SW620세포계중표체정황,응용면역공침정기술검측이자지간시부존재상호작용.결과 여암방정상조직상비,Gli1화β-catenin재대장암조직중균고표체[평균증장배수분별위42.69화72.11(P＜ 0.05)].여HCT116상비,Gli1여β-catenin재HT-29、DLDI급SW620 3주대장암병변정도고적세포중표체균승고[증장배수분별위52.54、17.23、5.54급.5.30、6.34、2.78(균P＜0.05)],이자적표체구유협동성.면역공침정결과현시Gli1여β-catenin량단백지간존재상호작용.결론 Gli1화β-catenin재대장암중협동표체,단백지간존재상호작용,제시Gli1、β-catenin공동삼여장암발생、발전조공,가능시Hedgehog、Wnt신호통로교호작용적일조도경,대대장암적예방치료가능유제시작용.
Objective To investigate the expression of Glil and β-catenin,which are central molecules of Hedgehog and Wnt signaling pathway,in colon cancer tissues and cell lines,and evaluate their relationship.Methods RT-PCR and Western blot were used to determine the expression of Glil and β-catenin in colon cancer tissues and cells.Interaction between endogenous Glil and endogenous 3-catenin was examined using immunoprecipitation method.Results Compared to corresponding normal tissues,the expression of Gli1 and β-catenin raised raising with one accord,the average growth rates were 42.69,72.11 respectively (P ＜ 0.05).In colon cancer cell lines,the expression levels of Glil and β-catenin increased,which were 52.54,17.23,5.54 and 5.30,6.34,2.78 (all P ＜ 0.05).Both of them were enhanced in three Wnt aberrant active cells visibly.The immunoprecipitation result indicated that there was an interaction between Glil and β-catenin in colon cancer cells.Conclusion The expression of Glil and β-catenin rise congruously in both colon cancer cells and tissues,and there is an interaction between them.This interaction between two key components of Hedgehog and Wnt signaling may suggest the possible crosstalk manner in these pathways.
