肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2013年
5期
309-311,315
,共4页
郭庚%章翔%蒯东%薛乃照%刘跃亭%郝解贺
郭庚%章翔%蒯東%薛迺照%劉躍亭%郝解賀
곽경%장상%괴동%설내조%류약정%학해하
神经胶质瘤%免疫组织化学%FRAT1%β-catenin%Wnt/β-catenin信号通路
神經膠質瘤%免疫組織化學%FRAT1%β-catenin%Wnt/β-catenin信號通路
신경효질류%면역조직화학%FRAT1%β-catenin%Wnt/β-catenin신호통로
Glioma%Immunohistochemistry%FRAT1%β-catenin%Wnt/β-catenin pathway
目的 探讨FRAT1、β-catenin蛋白在人类脑胶质瘤中的表达情况及两者关系,研究二者与临床病理分级的关系.方法 应用免疫组织化学方法检测FRAT1及β-catenin蛋白在84例人类脑胶质瘤组织和6例人正常脑组织中的表达水平.结果 人类脑胶质瘤组织中FRAT1与β-catenin蛋白表达阳性率分别为66.7％(56/84)和77.4％(65/84),人正常脑组织均未见FRAT1与β-catenin蛋白表达.在Ⅰ、Ⅱ、Ⅲ、Ⅳ级脑胶质瘤标本中,FRAT1免疫反应评分分别为1.25±1.77、2.64±3.13、4.63±3.17和6.52±3.42,与人正常脑组织相比,差异有统计学意义(P＜0.01),β-catenin分别为1.06±1.18、2.86±2.75、4.74±3.07和7.15±1.90,与人正常脑组织相比,差异有统计学意义(P＜0.01).FRAT1与β-catenin表达水平随着脑胶质瘤病理级别的增高而升高(r=0.55,P＜ 0.01；r=0.70,P＜O.01),且FRAT1与β-catenin表达水平呈正相关(r=0.77,P＜ 0.01).结论 FRAT1与β-catenin高表达可能与人类脑胶质瘤的发生、发展密切相关.为Wnt/β-catenin信号通路的研究开辟了新的领域,也为脑胶质瘤病理诊断和基因治疗提供了新的靶点.
目的 探討FRAT1、β-catenin蛋白在人類腦膠質瘤中的錶達情況及兩者關繫,研究二者與臨床病理分級的關繫.方法 應用免疫組織化學方法檢測FRAT1及β-catenin蛋白在84例人類腦膠質瘤組織和6例人正常腦組織中的錶達水平.結果 人類腦膠質瘤組織中FRAT1與β-catenin蛋白錶達暘性率分彆為66.7％(56/84)和77.4％(65/84),人正常腦組織均未見FRAT1與β-catenin蛋白錶達.在Ⅰ、Ⅱ、Ⅲ、Ⅳ級腦膠質瘤標本中,FRAT1免疫反應評分分彆為1.25±1.77、2.64±3.13、4.63±3.17和6.52±3.42,與人正常腦組織相比,差異有統計學意義(P＜0.01),β-catenin分彆為1.06±1.18、2.86±2.75、4.74±3.07和7.15±1.90,與人正常腦組織相比,差異有統計學意義(P＜0.01).FRAT1與β-catenin錶達水平隨著腦膠質瘤病理級彆的增高而升高(r=0.55,P＜ 0.01；r=0.70,P＜O.01),且FRAT1與β-catenin錶達水平呈正相關(r=0.77,P＜ 0.01).結論 FRAT1與β-catenin高錶達可能與人類腦膠質瘤的髮生、髮展密切相關.為Wnt/β-catenin信號通路的研究開闢瞭新的領域,也為腦膠質瘤病理診斷和基因治療提供瞭新的靶點.
목적 탐토FRAT1、β-catenin단백재인류뇌효질류중적표체정황급량자관계,연구이자여림상병리분급적관계.방법 응용면역조직화학방법검측FRAT1급β-catenin단백재84례인류뇌효질류조직화6례인정상뇌조직중적표체수평.결과 인류뇌효질류조직중FRAT1여β-catenin단백표체양성솔분별위66.7％(56/84)화77.4％(65/84),인정상뇌조직균미견FRAT1여β-catenin단백표체.재Ⅰ、Ⅱ、Ⅲ、Ⅳ급뇌효질류표본중,FRAT1면역반응평분분별위1.25±1.77、2.64±3.13、4.63±3.17화6.52±3.42,여인정상뇌조직상비,차이유통계학의의(P＜0.01),β-catenin분별위1.06±1.18、2.86±2.75、4.74±3.07화7.15±1.90,여인정상뇌조직상비,차이유통계학의의(P＜0.01).FRAT1여β-catenin표체수평수착뇌효질류병리급별적증고이승고(r=0.55,P＜ 0.01；r=0.70,P＜O.01),차FRAT1여β-catenin표체수평정정상관(r=0.77,P＜ 0.01).결론 FRAT1여β-catenin고표체가능여인류뇌효질류적발생、발전밀절상관.위Wnt/β-catenin신호통로적연구개벽료신적영역,야위뇌효질류병리진단화기인치료제공료신적파점.
Objective To investigate the expression of FRAT1 and β-catenin in human brain glioma,analyze the correlation between the expression and clinical pathological grades and the correlation of the two genes.Methods FRAT1 and β-catenin were detected by immunohistochemistry in 84 human brain glioma tissues and 6 human normal brain tissues.Results 66.7 ％ (56/84) and 77.4 ％ (65/84) of human brain glioma tissues expressed FRAT1 and β-catenin protein,whereas no FRAT1 and β-catenin protein expression was detected in human normal brain tissues.The expression levels of FRAT1 and ββ-catenin increased markedly with the ascending of pathologic grade of tumor specimens (r =0.55,P ＜ 0.01,r =0.70,P ＜ 0.01),there was a positive correlation between FRAT1 and β-catenin (r =0.77,P ＜ 0.01).Conclusion FRAT1 and β-catenin over-expression maybe closely related with occurrence and development of human brain gliomas.The results provide important supplements for the research of Wnt/β-catenin pathway.Meanwhile,FRAT1 may act as a valuable biomarker for molecular diagnosis of glioma and a potential target for gene therapy of glioma.
