肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2013年
7期
463-465
,共3页
黄建红%王艳峰%翟晋芳%石理%苏文%韩福才
黃建紅%王豔峰%翟晉芳%石理%囌文%韓福纔
황건홍%왕염봉%적진방%석리%소문%한복재
肺肿瘤%Th17细胞%IL-17+CD8+T细胞%肿瘤免疫
肺腫瘤%Th17細胞%IL-17+CD8+T細胞%腫瘤免疫
폐종류%Th17세포%IL-17+CD8+T세포%종류면역
Lung neoplasms%Th17 cells%IL-17-producing CD8+ T cells%Tumor immunity
目的 检测白细胞介素(IL)-17+CD4+T(Th17)细胞和IL-17+CD8+ T(Tc17)细胞在肺癌患者外周血中的表达水平,探讨二者在肺癌免疫中的作用及临床意义.方法 采用流式细胞术(FCM)检测60例肺癌患者及40例健康对照者外周血中Th17和Tc17细胞占CD;T细胞的比例.结果 肺癌组外周血中Th17细胞[(1.795±0.623)％]和Tc17细胞[(0.865±0.357)％]比例分别高于对照组[(1.405±0.256)％、(0.640±0.204)％],(t=28.944,P＜ 0.001;t=14.051,P＜ 0.001).两组内Th17细胞与Tc17细胞的表达水平均呈正相关(肺癌组r=0.770,P＜0.05；对照组r=0.532,P＜0.05).Th7细胞和Tc17细胞表达均与临床分期有关(F值分别为4.882、3.633,均P＜0.05),但与病理类型无关(均P＞0.05).结论 肺癌患者体内Th17细胞和Tc17细胞表达升高,二者可能参与了肺癌的发生、发展；Th17与Tc17细胞的表达水平可作为评价肺癌患者免疫功能状态的新指标,能为病情监测提供参考.
目的 檢測白細胞介素(IL)-17+CD4+T(Th17)細胞和IL-17+CD8+ T(Tc17)細胞在肺癌患者外週血中的錶達水平,探討二者在肺癌免疫中的作用及臨床意義.方法 採用流式細胞術(FCM)檢測60例肺癌患者及40例健康對照者外週血中Th17和Tc17細胞佔CD;T細胞的比例.結果 肺癌組外週血中Th17細胞[(1.795±0.623)％]和Tc17細胞[(0.865±0.357)％]比例分彆高于對照組[(1.405±0.256)％、(0.640±0.204)％],(t=28.944,P＜ 0.001;t=14.051,P＜ 0.001).兩組內Th17細胞與Tc17細胞的錶達水平均呈正相關(肺癌組r=0.770,P＜0.05；對照組r=0.532,P＜0.05).Th7細胞和Tc17細胞錶達均與臨床分期有關(F值分彆為4.882、3.633,均P＜0.05),但與病理類型無關(均P＞0.05).結論 肺癌患者體內Th17細胞和Tc17細胞錶達升高,二者可能參與瞭肺癌的髮生、髮展；Th17與Tc17細胞的錶達水平可作為評價肺癌患者免疫功能狀態的新指標,能為病情鑑測提供參攷.
목적 검측백세포개소(IL)-17+CD4+T(Th17)세포화IL-17+CD8+ T(Tc17)세포재폐암환자외주혈중적표체수평,탐토이자재폐암면역중적작용급림상의의.방법 채용류식세포술(FCM)검측60례폐암환자급40례건강대조자외주혈중Th17화Tc17세포점CD;T세포적비례.결과 폐암조외주혈중Th17세포[(1.795±0.623)％]화Tc17세포[(0.865±0.357)％]비례분별고우대조조[(1.405±0.256)％、(0.640±0.204)％],(t=28.944,P＜ 0.001;t=14.051,P＜ 0.001).량조내Th17세포여Tc17세포적표체수평균정정상관(폐암조r=0.770,P＜0.05；대조조r=0.532,P＜0.05).Th7세포화Tc17세포표체균여림상분기유관(F치분별위4.882、3.633,균P＜0.05),단여병리류형무관(균P＞0.05).결론 폐암환자체내Th17세포화Tc17세포표체승고,이자가능삼여료폐암적발생、발전；Th17여Tc17세포적표체수평가작위평개폐암환자면역공능상태적신지표,능위병정감측제공삼고.
Objective To investigate the expression and clinical significance of Th17 and Tc17 cells in peripheral blood of lung cancer patients.Methods The percentages of Th17 cells and Tc17 cells in 60 lung cancer patients and 40 healthy controls were evaluated by flow cytometry analysis (FCM).Results The percentages of Th17 cells [(1.795±0.623) ％] and Te17 cells [(0.865±0.357) ％] in lung cancer group were significandy higher than those in controls [(1.405±0.256) ％,(0.640 ±0.204) ％],(t =28.944,P ＜ 0.001,t =14.051,P ＜ 0.001).Furthermore,there was a positive correlation between Th17 cells and Tc17 cells in the two groups (lung cancer group r =0.770,P ＜ 0.05,control group r =0.532,P ＜ 0.05).The percentages of Th17 cells and Tc17 cells were closely associated with clinical stage (F =4.882,P =0.011,F =3.633,P =0.033),but not connected with pathological types (P ＞ 0.05,P ＞ 0.05).Conclusion The overexpression of Th17 and Tc17 may be involved in the occurrence and development of lung cancer,which can be used as new indicators for immunologic function of lung cancer patients,and provide a reference in monitoring the disease.
