肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2013年
9期
595-598
,共4页
杨东杰%姚宏%茅青%冯晓星%谢碧琛
楊東傑%姚宏%茅青%馮曉星%謝碧琛
양동걸%요굉%모청%풍효성%사벽침
胃肿瘤%多药耐药%谷胱甘肽转移酶π%P-糖蛋白%拓扑异构酶Ⅱ%胸苷酸合成酶
胃腫瘤%多藥耐藥%穀胱甘肽轉移酶π%P-糖蛋白%拓撲異構酶Ⅱ%胸苷痠閤成酶
위종류%다약내약%곡광감태전이매π%P-당단백%탁복이구매Ⅱ%흉감산합성매
Stomach neoplasms%Multidrug resistance%Glutathione-S-transferase-π%P-glycoprotein%Topoisomerase Ⅱ%Thymidylate synthase
目的 探讨胎盘型谷胱甘肽转移酶π(GST-π)、P-糖蛋白(P-gp)、拓扑异构酶Ⅱ(TopoⅡ)及胸苷酸合成酶(TS)在胃腺癌组织中的表达特点及其临床意义.方法 应用免疫组织化学Envision法联合检测GST-π、P-gp、TopoⅡ及TS在64例胃腺癌组织和12例正常胃黏膜组织中的表达,并结合其临床资料进行综合分析.结果 胃腺癌组织中GST-π、P-gp、TopoⅡ及TS的表达阳性率分别为76.6％(49/64)、64.1％(41/64)、84.4％(50/64)和53.1％(34/64),均高于其在正常胃黏膜组织中的8.3％(1/12)、8.3％(1/12)、16.7％(2/12)、0,其差异均有统计学意义(P＜0.01)；它们在胃腺癌组织中的表达与患者的性别、年龄、肿瘤大小、临床分期、侵袭深度及淋巴结转移情况均无关(P＞0.05),而与肿瘤的分化程度有关(P＜0.05),GST-π、P-gp及TS在高、中分化腺癌中的表达均高于低分化腺癌,而TopoⅡ在高、中分化腺癌中的表达低于低分化腺癌(P＜0.05).结论 GST-π、P-gp、TopoⅡ及TS在胃腺癌中过表达与胃腺癌的多药耐药相关.联合检测GST-π、P-gp、TopoⅡ及TS在胃腺癌组织中的表达,对化疗药物的选择及预后的判断有一定的临床指导意义.
目的 探討胎盤型穀胱甘肽轉移酶π(GST-π)、P-糖蛋白(P-gp)、拓撲異構酶Ⅱ(TopoⅡ)及胸苷痠閤成酶(TS)在胃腺癌組織中的錶達特點及其臨床意義.方法 應用免疫組織化學Envision法聯閤檢測GST-π、P-gp、TopoⅡ及TS在64例胃腺癌組織和12例正常胃黏膜組織中的錶達,併結閤其臨床資料進行綜閤分析.結果 胃腺癌組織中GST-π、P-gp、TopoⅡ及TS的錶達暘性率分彆為76.6％(49/64)、64.1％(41/64)、84.4％(50/64)和53.1％(34/64),均高于其在正常胃黏膜組織中的8.3％(1/12)、8.3％(1/12)、16.7％(2/12)、0,其差異均有統計學意義(P＜0.01)；它們在胃腺癌組織中的錶達與患者的性彆、年齡、腫瘤大小、臨床分期、侵襲深度及淋巴結轉移情況均無關(P＞0.05),而與腫瘤的分化程度有關(P＜0.05),GST-π、P-gp及TS在高、中分化腺癌中的錶達均高于低分化腺癌,而TopoⅡ在高、中分化腺癌中的錶達低于低分化腺癌(P＜0.05).結論 GST-π、P-gp、TopoⅡ及TS在胃腺癌中過錶達與胃腺癌的多藥耐藥相關.聯閤檢測GST-π、P-gp、TopoⅡ及TS在胃腺癌組織中的錶達,對化療藥物的選擇及預後的判斷有一定的臨床指導意義.
목적 탐토태반형곡광감태전이매π(GST-π)、P-당단백(P-gp)、탁복이구매Ⅱ(TopoⅡ)급흉감산합성매(TS)재위선암조직중적표체특점급기림상의의.방법 응용면역조직화학Envision법연합검측GST-π、P-gp、TopoⅡ급TS재64례위선암조직화12례정상위점막조직중적표체,병결합기림상자료진행종합분석.결과 위선암조직중GST-π、P-gp、TopoⅡ급TS적표체양성솔분별위76.6％(49/64)、64.1％(41/64)、84.4％(50/64)화53.1％(34/64),균고우기재정상위점막조직중적8.3％(1/12)、8.3％(1/12)、16.7％(2/12)、0,기차이균유통계학의의(P＜0.01)；타문재위선암조직중적표체여환자적성별、년령、종류대소、림상분기、침습심도급림파결전이정황균무관(P＞0.05),이여종류적분화정도유관(P＜0.05),GST-π、P-gp급TS재고、중분화선암중적표체균고우저분화선암,이TopoⅡ재고、중분화선암중적표체저우저분화선암(P＜0.05).결론 GST-π、P-gp、TopoⅡ급TS재위선암중과표체여위선암적다약내약상관.연합검측GST-π、P-gp、TopoⅡ급TS재위선암조직중적표체,대화료약물적선택급예후적판단유일정적림상지도의의.
Objective To explore the expression of glutathione-S-transferase-π(GST-π),P-glycoprotein (P-gp),topoisomerase Ⅱ (Topo Ⅱ) and thymidylate synthase (TS) in gastric adenocarcinoma tissues and the clinical significance.Methods Envision method of immunohistochemistry was used to detect the expression of GST-π,P-gp,Topo Ⅱ and TS in sample of 64 gastric adenocarcinoma tissues and 12 normal gastric mucosas,and their corresponding clinical data were comprehensive analyzed.Results The expression positive rates of GST-π,P-gp,Topo Ⅱand TS were respectively 76.6 ％(49/64),64.1％ (41/64),84.4 ％ (50/64) and 53.1％ (34/64),that were all higher than in gastric mucosa [8.3 ％ (1/12),8.3 ％(1/12),16.7 ％ (2/12),0],the differences were statistically significant respectively (P ＜ 0.01).Their positive expression rates were closely relevant to the degree of differentiation (P ＜ 0.05),but not to the patients' gender,age,tumour size,clinical staging,invasive depth and lymph node metastasis (P ＞ 0.05).The expressions of GST-π,P-gp and TS in high and middle differentiation adenocarcinoma were high.er than in low differentiation,but the expression of Topo Ⅱ in high and middle differentiation adenocarcinoma was lower than in low differentiation (P ＜ 0.05).Conclusion GST-π,P-gp,Topo Ⅱ and TS are over-expressed in gastric adenocarcinoma,which is related to the multidrug resistance of gastric adenocarcinoma.The joint detection of the expression of GST-π,P-gp,Topo Ⅱ and TS in gastric adenocarcinoma can be looked as an important symbol for guiding its chemotherapy drug and prognosis.
