肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2013年
9期
599-601
,共3页
庄晓飞%张弘广%王春利%郭石平%马炎炎%解立武
莊曉飛%張弘廣%王春利%郭石平%馬炎炎%解立武
장효비%장홍엄%왕춘리%곽석평%마염염%해립무
食管肿瘤%反转录聚合酶链反应%PED/PEA-15%免疫组织化学
食管腫瘤%反轉錄聚閤酶鏈反應%PED/PEA-15%免疫組織化學
식관종류%반전록취합매련반응%PED/PEA-15%면역조직화학
Esophageal neoplasms%Reverse transcriptase polymerase chain reaction%PED/PEA-15%Immunohistochemistry
目的 探讨PED/PEA-15 mRNA及蛋白在食管癌组织中的表达情况及其临床意义.方法 应用半定量反转录-聚合酶链反应(RT-PCR)检测50例食管癌、相应癌旁组织和正常食管组织中PED/PEA-15基因的相对表达量,并通过免疫组织化学检测PED/PEA-15蛋白的表达.结果 半定量RT-PCR显示,PED/PEA-15基因在食管癌组织中均呈高表达,癌旁组织及正常食管组织呈低表达或不表达,相对表达量分别为1.14±0.49、0.59±0.31和0.53±0.22,PED/PEA-15 mRNA在食管癌组织中表达较癌旁组织、正常食管组织升高(F=44.085,P＜0.01).免疫组织化学结果显示,食管癌组织中PED/PEA-15蛋白阳性表达率高于正常食管组织和癌旁组织(x2=36.967,P＜0.001；x2=26.272,P＜ 0.001).且PED/PEA-15 mRNA及蛋白的表达与食管癌病理分级、临床分期有关(P＜0.05),而与发病年龄、性别、病理类型无相关性(P＞0.05).结论 PED/PEA-15 mRNA及蛋白在食管癌组织中表达水平明显升高,可能在食管癌的发生及发展过程中发挥重要作用.
目的 探討PED/PEA-15 mRNA及蛋白在食管癌組織中的錶達情況及其臨床意義.方法 應用半定量反轉錄-聚閤酶鏈反應(RT-PCR)檢測50例食管癌、相應癌徬組織和正常食管組織中PED/PEA-15基因的相對錶達量,併通過免疫組織化學檢測PED/PEA-15蛋白的錶達.結果 半定量RT-PCR顯示,PED/PEA-15基因在食管癌組織中均呈高錶達,癌徬組織及正常食管組織呈低錶達或不錶達,相對錶達量分彆為1.14±0.49、0.59±0.31和0.53±0.22,PED/PEA-15 mRNA在食管癌組織中錶達較癌徬組織、正常食管組織升高(F=44.085,P＜0.01).免疫組織化學結果顯示,食管癌組織中PED/PEA-15蛋白暘性錶達率高于正常食管組織和癌徬組織(x2=36.967,P＜0.001；x2=26.272,P＜ 0.001).且PED/PEA-15 mRNA及蛋白的錶達與食管癌病理分級、臨床分期有關(P＜0.05),而與髮病年齡、性彆、病理類型無相關性(P＞0.05).結論 PED/PEA-15 mRNA及蛋白在食管癌組織中錶達水平明顯升高,可能在食管癌的髮生及髮展過程中髮揮重要作用.
목적 탐토PED/PEA-15 mRNA급단백재식관암조직중적표체정황급기림상의의.방법 응용반정량반전록-취합매련반응(RT-PCR)검측50례식관암、상응암방조직화정상식관조직중PED/PEA-15기인적상대표체량,병통과면역조직화학검측PED/PEA-15단백적표체.결과 반정량RT-PCR현시,PED/PEA-15기인재식관암조직중균정고표체,암방조직급정상식관조직정저표체혹불표체,상대표체량분별위1.14±0.49、0.59±0.31화0.53±0.22,PED/PEA-15 mRNA재식관암조직중표체교암방조직、정상식관조직승고(F=44.085,P＜0.01).면역조직화학결과현시,식관암조직중PED/PEA-15단백양성표체솔고우정상식관조직화암방조직(x2=36.967,P＜0.001；x2=26.272,P＜ 0.001).차PED/PEA-15 mRNA급단백적표체여식관암병리분급、림상분기유관(P＜0.05),이여발병년령、성별、병리류형무상관성(P＞0.05).결론 PED/PEA-15 mRNA급단백재식관암조직중표체수평명현승고,가능재식관암적발생급발전과정중발휘중요작용.
Objective To investigate the expression of PED/PEA-15 mRNA and protein in esophageal carcinoma tissue and their clinical significances.Methods The expression of PED/PEA-15 mRNA was detected by RT-PCR,and the expression of PED/PEA-15 protein was measured by immunohistochemistry in 50 cases of esophageal carcinoma,50 cases of corresponding paracarcinoma tissue,and 50 cases of corresponding normal esophageal tissue.Results The expression of PED/PEA-15 mRNA was 1.14±0.49 in esophageal carcinoma,which was significantly higher than that in para-carcinoma tissue (0.59±0.31) and normal esophageal carcinoma tissue (0.53±0.22) (F =44.085,P ＜ 0.001).The immunohistochemistry results showed that PED/PEA-15 protein expression was significantly higher than that in para-carcinoma tissue and normal esophageal tissue (x2 =36.967,P ＜ 0.001; x2 =26.272,P ＜ 0.001).The expression of PED/PEA-15 mRNA and protein were significantly associated with pathological grade,clinical stage of esophageal carcinoma (P ＜ 0.05),but were not significantly correlated to the age of onset,gender,pathological types (P ＞ 0.05).Conclusion The expression of PED/PEA-15 mRNA and protein are increased in esophageal carcinoma,which may play an important role in the occurrence and development of esophageal carcinoma.
