肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2013年
12期
816-818,822
,共4页
李凤立%马迎春%陈海英%王爱霞%靳卫国%陈双峰
李鳳立%馬迎春%陳海英%王愛霞%靳衛國%陳雙峰
리봉립%마영춘%진해영%왕애하%근위국%진쌍봉
卵巢肿瘤%囊腺癌,浆液%高尔基磷酸化蛋白3%聚合酶链反应%印迹法,蛋白质
卵巢腫瘤%囊腺癌,漿液%高爾基燐痠化蛋白3%聚閤酶鏈反應%印跡法,蛋白質
란소종류%낭선암,장액%고이기린산화단백3%취합매련반응%인적법,단백질
Ovarian neoplasms%Cystadenocarcinoma,serous%Golgi phosphoprotein 3%Polymerase chain reaction%Blotting,western
目的 研究高尔基磷酸化蛋白3(GOLPH3)在卵巢浆液性囊腺癌组织中的表达及意义.方法 应用实时荧光定量PCR、Western blot分别检测正常卵巢组织、卵巢浆液性囊腺瘤、卵巢浆液性囊腺癌组织中GOLPH3在mRNA和蛋白水平的表达.结果 GOLPH3 mRNA在卵巢浆液性囊腺瘤和浆液性囊腺癌组织中均有表达,卵巢浆液性囊腺癌组织中表达高于良性肿瘤组(2.97～7.04倍),差异有统计学意义(P＜0.05).GOLPH3蛋白在正常卵巢组织中未检测到表达,卵巢浆液性囊腺癌组织中阳性表达率73.8％,明显高于良性肿瘤组的35.71％(P＜0.01).GOLPH3蛋白阳性表达率与浆液性囊腺癌的临床分期(P=0.019)、病理分级(P=0.042)及有无淋巴结转移有关(P=0.000),与患者年龄无关(P＞0.05);且肿瘤分化越低GOLPH3蛋白表达量越高(P＜0.01).结论 GOLPH3的过表达在卵巢浆液性囊腺癌的发生、发展中意义重大,可能是卵巢癌早期诊断和临床治疗的潜在靶点.
目的 研究高爾基燐痠化蛋白3(GOLPH3)在卵巢漿液性囊腺癌組織中的錶達及意義.方法 應用實時熒光定量PCR、Western blot分彆檢測正常卵巢組織、卵巢漿液性囊腺瘤、卵巢漿液性囊腺癌組織中GOLPH3在mRNA和蛋白水平的錶達.結果 GOLPH3 mRNA在卵巢漿液性囊腺瘤和漿液性囊腺癌組織中均有錶達,卵巢漿液性囊腺癌組織中錶達高于良性腫瘤組(2.97～7.04倍),差異有統計學意義(P＜0.05).GOLPH3蛋白在正常卵巢組織中未檢測到錶達,卵巢漿液性囊腺癌組織中暘性錶達率73.8％,明顯高于良性腫瘤組的35.71％(P＜0.01).GOLPH3蛋白暘性錶達率與漿液性囊腺癌的臨床分期(P=0.019)、病理分級(P=0.042)及有無淋巴結轉移有關(P=0.000),與患者年齡無關(P＞0.05);且腫瘤分化越低GOLPH3蛋白錶達量越高(P＜0.01).結論 GOLPH3的過錶達在卵巢漿液性囊腺癌的髮生、髮展中意義重大,可能是卵巢癌早期診斷和臨床治療的潛在靶點.
목적 연구고이기린산화단백3(GOLPH3)재란소장액성낭선암조직중적표체급의의.방법 응용실시형광정량PCR、Western blot분별검측정상란소조직、란소장액성낭선류、란소장액성낭선암조직중GOLPH3재mRNA화단백수평적표체.결과 GOLPH3 mRNA재란소장액성낭선류화장액성낭선암조직중균유표체,란소장액성낭선암조직중표체고우량성종류조(2.97～7.04배),차이유통계학의의(P＜0.05).GOLPH3단백재정상란소조직중미검측도표체,란소장액성낭선암조직중양성표체솔73.8％,명현고우량성종류조적35.71％(P＜0.01).GOLPH3단백양성표체솔여장액성낭선암적림상분기(P=0.019)、병리분급(P=0.042)급유무림파결전이유관(P=0.000),여환자년령무관(P＞0.05);차종류분화월저GOLPH3단백표체량월고(P＜0.01).결론 GOLPH3적과표체재란소장액성낭선암적발생、발전중의의중대,가능시란소암조기진단화림상치료적잠재파점.
Objective To investigate the expression of Golgi phosphoprotein 3 (GOLPH3) at protein and mRNA levels in serous epitheial ovarian carcinoma and its significance.Methods The expression of GOLPH3 at protein and mRNA levels were evaluaed by Western blot and Real time RT-PCR,respectively,in 42 cases of serous epithelium ovarian carcinoma,14 cases of benign serous ovarian tumors and 7 cases of normal ovarian epithelium tissues.Results GOLPH3 mRNA was significantly higher from 2.97 to 7.04 fold in ovarian serous cystadenocarcinoma tissues compared with ovarian serous carcinoma tissues (P ＜ 0.05).There was no positive expression of GOLPH3 protein in normal ovarian epithelium tissues.GOLPH3 protein positive expression rate was higher in ovarian serous cystadenocarcinoma (73.81 ％) than that in ovarian serous carcinoma (35.71％) (P ＜ 0.01).GOLPH3 protein was correlated with the pathologic differentiation (P =0.019),the FIGO stage (P =0.042),and lymphatic metastasis (P =0.000),but was not associated with age (P =0,881).The positive rate and overexpression of GOLPH3 in poor differentiated grade group were higher than that in well and middle differentiated grade group (P ＜ 0.05).Conclusions GOLPH3 may play an important role on the development of ovarian serous cystadenocarcinoma,and may be a potential target of gene therapy.
