肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2013年
12期
823-825
,共3页
胃肿瘤%上皮-间质转化%Twist转录因子
胃腫瘤%上皮-間質轉化%Twist轉錄因子
위종류%상피-간질전화%Twist전록인자
Stomach neoplasms%Epithelial-mesenchymal transition%Twist transcription factor
目的 分析胃腺癌组织中Twist蛋白的表达与临床病理参数之间的相关性及其与上皮-间质转化(EMT)的关系,探讨Twist的表达在判断患者预后中的意义.方法 采用免疫组织化学SABC法检测120例胃腺癌标本及其对应癌旁组织中Twist及EMT相关蛋白上皮钙黏素(E-cad)、神经钙黏素(N-cad)的表达情况,统计分析Twist表达与患者临床病理参数的关系及其与E-cad及N-cad表达的相关性.结果 在胃腺癌组织中Twist、E-cad、N-cad阳性率分别为59.1％(71/120)、36.6％(44/120)、47.5％(57/120),癌旁组织中阳性率分别为17.5％(21/120)、93.3％(112/120)、11.6％(14/120),两种组织间各指标阳性率差异均有统计学意义(均P＜0.05).Twist的表达与肿瘤远处转移、淋巴结转移相关,且与浸润深度呈正相关(均P＜ 0.05).Twist的表达与N-cad和E-cad的表达存在相关性(P＜0.05).结论 胃腺癌组织中Twist、E-cad、N-cad的表达与癌旁组织明显不同,并且胃腺癌组织中Twist的表达与肿瘤的生物学特征密切相关;胃癌组织中Twist蛋白表达与EMT相关蛋白E-cad表达呈负相关,而与N-cad表达呈正相关.
目的 分析胃腺癌組織中Twist蛋白的錶達與臨床病理參數之間的相關性及其與上皮-間質轉化(EMT)的關繫,探討Twist的錶達在判斷患者預後中的意義.方法 採用免疫組織化學SABC法檢測120例胃腺癌標本及其對應癌徬組織中Twist及EMT相關蛋白上皮鈣黏素(E-cad)、神經鈣黏素(N-cad)的錶達情況,統計分析Twist錶達與患者臨床病理參數的關繫及其與E-cad及N-cad錶達的相關性.結果 在胃腺癌組織中Twist、E-cad、N-cad暘性率分彆為59.1％(71/120)、36.6％(44/120)、47.5％(57/120),癌徬組織中暘性率分彆為17.5％(21/120)、93.3％(112/120)、11.6％(14/120),兩種組織間各指標暘性率差異均有統計學意義(均P＜0.05).Twist的錶達與腫瘤遠處轉移、淋巴結轉移相關,且與浸潤深度呈正相關(均P＜ 0.05).Twist的錶達與N-cad和E-cad的錶達存在相關性(P＜0.05).結論 胃腺癌組織中Twist、E-cad、N-cad的錶達與癌徬組織明顯不同,併且胃腺癌組織中Twist的錶達與腫瘤的生物學特徵密切相關;胃癌組織中Twist蛋白錶達與EMT相關蛋白E-cad錶達呈負相關,而與N-cad錶達呈正相關.
목적 분석위선암조직중Twist단백적표체여림상병리삼수지간적상관성급기여상피-간질전화(EMT)적관계,탐토Twist적표체재판단환자예후중적의의.방법 채용면역조직화학SABC법검측120례위선암표본급기대응암방조직중Twist급EMT상관단백상피개점소(E-cad)、신경개점소(N-cad)적표체정황,통계분석Twist표체여환자림상병리삼수적관계급기여E-cad급N-cad표체적상관성.결과 재위선암조직중Twist、E-cad、N-cad양성솔분별위59.1％(71/120)、36.6％(44/120)、47.5％(57/120),암방조직중양성솔분별위17.5％(21/120)、93.3％(112/120)、11.6％(14/120),량충조직간각지표양성솔차이균유통계학의의(균P＜0.05).Twist적표체여종류원처전이、림파결전이상관,차여침윤심도정정상관(균P＜ 0.05).Twist적표체여N-cad화E-cad적표체존재상관성(P＜0.05).결론 위선암조직중Twist、E-cad、N-cad적표체여암방조직명현불동,병차위선암조직중Twist적표체여종류적생물학특정밀절상관;위암조직중Twist단백표체여EMT상관단백E-cad표체정부상관,이여N-cad표체정정상관.
Objective To investigate the relationship between the expressions of Twist and clinical pathological parameters in gastric carcinoma,to explore its significance in judging the prognosis of patients,and to analyze the expression of Twist proteins in the epithelial-mesenchymal transition (EMT) phenomenon.Methods Immunohistochemistry was used to detect the expressions of Twist and EMT relative proteins E-cad,N-cad in 120 cases of gastric carcinoma specimens and corresponding adjacent normal tissue.Analysis The relationship between Twist expression and clinical pathological parameters,and the Twist expression and E-cad,N-cad were analyzed.Results In gastric carcinoma,Twist,E-cad,N-cad positive rates were 59.1％(71/120),36.6 ％ (44/120) and 47.5 ％ (57/120),and in adjacent tissues,the positive rate were 17.5 ％ (21/120),93.3 ％ (112/120) and 11.6 ％ (14/120).There were significant differences in each index between tumor tissues and adjacent tissues (P ＜ 0.05).Patients with Twist positive expression showed higher incidence rates of lymph node metastasis (P ＜ 0.05) and distant metastasis (P ＜ 0.05) than those with Twist negative expression.The expression of Twist was significantly correlated in gastric carcinoma accompanied by low expression of E-cad and abnormal high N-cad expression (P ＜ 0.05).Conclusions Compared with the adjacent tissue,the expression of Twist,E-cad and N-cad have significant differences,and the expression of Twist was closely related to the biological characteristics in gastric carcinoma.The expression of Twist in gastric cancer have negative correlation with the EMT relative factor E-cad,while positive correlation with the N-cad.
