肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2014年
2期
94-97
,共4页
林桂淼%李慧%林李家宓%林苏霞%吴世明%王晓梅
林桂淼%李慧%林李傢宓%林囌霞%吳世明%王曉梅
림계묘%리혜%림리가복%림소하%오세명%왕효매
鼻咽肿瘤%hTERTC27%端粒%末端转移酶%基因疗法
鼻嚥腫瘤%hTERTC27%耑粒%末耑轉移酶%基因療法
비인종류%hTERTC27%단립%말단전이매%기인요법
Nasopharyngeal neoplasms%hTERTC27%Telomere%Terminal transferase%Gene therapy
目的 研究人类端粒酶反转录酶C27多肽(hTERTC27)过表达对鼻咽癌C666-1细胞移植瘤生长的影响.方法 利用稳定转染质粒pcDNA3.1(对照组)与pcDNA3.1-hTERTC27(hTERTC27组)的C666-1细胞株建立裸鼠鼻咽癌皮下移植瘤模型.观察肿瘤生长情况,绘制肿瘤生长曲线,处死后剥离肿瘤,进行HE染色观察组织形态学变化.采用Western blot法观察肿瘤组织中剪切后的Caspase-3、Caspase-9、PARP以及bax和bcl-2的表达.结果 小鼠荷瘤第8天后,hTERTC27组移植瘤的体积明显小于对照组(P＜0.05),肿瘤的重量明显低于对照组(P＜0.05),局部肌肉浸润现象较少.hTERTC27组移植瘤Caspase-3、Caspase-9、PARP、bax的表达量增加(P＜0.01),而bcl-2的表达量降低(P＜0.01).结论 hTERTC27减缓裸鼠鼻咽癌皮下移植瘤的生长速度并促进其凋亡,可能与其上调hax蛋白的表达和下调bcl-2蛋白的表达有关.
目的 研究人類耑粒酶反轉錄酶C27多肽(hTERTC27)過錶達對鼻嚥癌C666-1細胞移植瘤生長的影響.方法 利用穩定轉染質粒pcDNA3.1(對照組)與pcDNA3.1-hTERTC27(hTERTC27組)的C666-1細胞株建立裸鼠鼻嚥癌皮下移植瘤模型.觀察腫瘤生長情況,繪製腫瘤生長麯線,處死後剝離腫瘤,進行HE染色觀察組織形態學變化.採用Western blot法觀察腫瘤組織中剪切後的Caspase-3、Caspase-9、PARP以及bax和bcl-2的錶達.結果 小鼠荷瘤第8天後,hTERTC27組移植瘤的體積明顯小于對照組(P＜0.05),腫瘤的重量明顯低于對照組(P＜0.05),跼部肌肉浸潤現象較少.hTERTC27組移植瘤Caspase-3、Caspase-9、PARP、bax的錶達量增加(P＜0.01),而bcl-2的錶達量降低(P＜0.01).結論 hTERTC27減緩裸鼠鼻嚥癌皮下移植瘤的生長速度併促進其凋亡,可能與其上調hax蛋白的錶達和下調bcl-2蛋白的錶達有關.
목적 연구인류단립매반전록매C27다태(hTERTC27)과표체대비인암C666-1세포이식류생장적영향.방법 이용은정전염질립pcDNA3.1(대조조)여pcDNA3.1-hTERTC27(hTERTC27조)적C666-1세포주건립라서비인암피하이식류모형.관찰종류생장정황,회제종류생장곡선,처사후박리종류,진행HE염색관찰조직형태학변화.채용Western blot법관찰종류조직중전절후적Caspase-3、Caspase-9、PARP이급bax화bcl-2적표체.결과 소서하류제8천후,hTERTC27조이식류적체적명현소우대조조(P＜0.05),종류적중량명현저우대조조(P＜0.05),국부기육침윤현상교소.hTERTC27조이식류Caspase-3、Caspase-9、PARP、bax적표체량증가(P＜0.01),이bcl-2적표체량강저(P＜0.01).결론 hTERTC27감완라서비인암피하이식류적생장속도병촉진기조망,가능여기상조hax단백적표체화하조bcl-2단백적표체유관.
Objective To investigate the effect of human telomerase reverse transcriptase C27 polypeptide (hTERTC27) over-expression on orthotopic implanted tumor growth which induced by nasopharyngeal carcinoma cells C666-1 in vivo.Methods Stably transfected C666-1 cell lines were used to establish subcutaneously transplanted tumor mouse model.The tumor growth was observed and the tumor growth curve was made by measuring the volumes of tumors every day.HE staining was used to observe the change of histomorphology.The expressions of cleaved Caspase-3,Caspase-9,PARP,bax and bcl-2 were detected by Western blot analysis.Results The in vivo mouse model showed that over-expression of hTERTC27 significantly reduced tumor volume and tumor weight (P ＜ 0.05),and decreased the local muscle infiltration.Over-expression of hTERTC27 significantly up-regulated the expressions of cleaved Caspase-3,Caspase-9,PARP and bax (P ＜ 0.01),and down-regulated the expression of bcl-2 (P ＜ 0.01).Conclusion The results indicate that over-expression of hTERTC27 can obviously inhibit the growth of transplanted tumor in nude mice,which is possibly related to the regulation of bax and bcl-2 expression.
