CAJ | 학술논문

目的探讨乳腺癌和癌旁组织中雌激素受体(ER)β亚型构成比变化.方法收集87例乳腺癌患者癌和癌旁组织,采用实时荧光定量聚合酶链反应定量测定成对组织中ERβ亚型(ERβ 1、ERβ2和ERβ5)相对表达水平,最终获得各亚型在癌变中的比例.结果癌组织中ERβ 1、ERβ2和ERβ5的表达水平均较癌旁组织低(P=0.00).在癌组织和癌旁组织,ERβ5在3种主要亚型中所占阳性比例均最高(54.02％和75.84％),尤以癌旁组织为甚,而ERβ 1阳性比例均最低(6.77％和9.74％).ERβ 1和ERβ2在癌旁组织中阳性比例低于癌组织(Z=-2.24,P=0.025和Z=-4.85,P＜0.01),而ERβ5在癌旁组织中阳性比例明显高于癌组织(Z=-5.32,P＜0.01).结论乳腺癌变过程中ERβ各亚型表达水平均明显下调,但下调幅度差异显著,亚型构成比变化明显,提示ERβ各亚型在癌变过程中表达调控的差异性机制将成为乳腺癌发病机制研究新的突破口.
목적 탐토유선암화암방조직중자격소수체(ER)β아형구성비변화.방법 수집87례유선암환자암화암방조직,채용실시형광정량취합매련반응정량측정성대조직중ERβ아형(ERβ 1、ERβ2화ERβ5)상대표체수평,최종획득각아형재암변중적비례.결과 암조직중ERβ 1、ERβ2화ERβ5적표체수평균교암방조직저(P=0.00).재암조직화암방조직,ERβ5재3충주요아형중소점양성비례균최고(54.02％화75.84％),우이암방조직위심,이ERβ 1양성비례균최저(6.77％화9.74％).ERβ 1화ERβ2재암방조직중양성비례저우암조직(Z=-2.24,P=0.025화Z=-4.85,P＜0.01),이ERβ5재암방조직중양성비례명현고우암조직(Z=-5.32,P＜0.01).결론 유선암변과정중ERβ각아형표체수평균명현하조,단하조폭도차이현저,아형구성비변화명현,제시ERβ각아형재암변과정중표체조공적차이성궤제장성위유선암발병궤제연구신적돌파구.
Objective To illustrate the composition ratio of ERβ isoforms in paired cancerous and adjacent normal tissues from breast cancer patients.Methods Eighty-seven pairs of cancerous and adjacent normal tissues were obtained from breast cancer patients.RT-qPCR was used to determine the relative mRNA expression levels of ERβ isoforms (ER[β1,ERβ2 and ERβ5),and the composition ratios of ERβ isoforms were analyzed.Results The expression levels of all tested ERβ isoforms (ERβ1,ERβ2 and ERβ5) in breast cancer tissues were much lower than those in adjacent normal breast tissues (P ＜ 0.01).Isoform ratio analysis showed that ERβ5 was the dominant isoform in both cancerous and adjacent normal tissues with a positive detection rate of 54.02 ％ and 75.84 ％,respectively.Meanwhile,ERβ1 had the lowest detection rate (9.74 ％ and 6.77 ％ in cancerous and adjacent normal tissues,respectively).The positive rates for both ERβ1 and ERβ2 were much lower in adjacent normal tissues than those in cancer tissues (Z =-2.24,P =0.025 and Z =-4.85,P ＜ 0.01,separately),while more cancerous tissues were ERβ5-positive in comparison to adjacent normal tissues (Z =-5.32,P ＜ 0.01).Conclusions The expression levels of all the ERβ isoforms are differentially down-regulated with significant alterations in their composition ratios during breast carcinogenesis.Further understanding on molecular mechanisms underlying the differential down-regulation of ER[β isoforms will shed new light on breast carcinogenesis.