肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2014年
4期
246-249
,共4页
王建纲%邵泉%王英斌%万远廉
王建綱%邵泉%王英斌%萬遠廉
왕건강%소천%왕영빈%만원렴
基因,肝癌缺失-1%结肠肿瘤%肿瘤分期%淋巴转移
基因,肝癌缺失-1%結腸腫瘤%腫瘤分期%淋巴轉移
기인,간암결실-1%결장종류%종류분기%림파전이
Gene,deleted in liver cancer-1%Colonic neoplasms%Neoplasm staging%Lymphatic metastasis
目的 探讨肝癌缺失-1(DLC-1)基因mRNA表达与结肠癌分期及淋巴结转移的相关性.方法 收集2009年9月至2011年9月手术切除结肠癌标本共60例,以外伤、结肠憩室、结肠炎手术治疗留取的正常组织10例为对照.采用反转录PCR定量检测结肠癌组织、癌旁组织、正常组织DLC-1基因mRNA的表达.依据TNM分期进行分期,采用直线相关分析DLC-1基因表达与结肠癌分期及淋巴结转移的相关性.结果 结肠癌组织DLC-1基因mRNA的表达水平(0.085±0.004)明显低于癌旁组织(0.562±0.063),癌旁组织低于正常组织(1.000±0.003).DLC-1基因mRNA表达水平与结肠癌分期呈负相关(r=0.51,P＜ 0.01),DLC-1基因mRNA表达水平与淋巴结转移呈负相关(r=0.46,P＜ 0.05).结论 DLC-1基因mRNA表达与病理学分期、淋巴结转移率呈负相关,DLC-1基因在结肠癌发生、发展中扮演重要角色,可能是研究结肠癌新的突破口.
目的 探討肝癌缺失-1(DLC-1)基因mRNA錶達與結腸癌分期及淋巴結轉移的相關性.方法 收集2009年9月至2011年9月手術切除結腸癌標本共60例,以外傷、結腸憩室、結腸炎手術治療留取的正常組織10例為對照.採用反轉錄PCR定量檢測結腸癌組織、癌徬組織、正常組織DLC-1基因mRNA的錶達.依據TNM分期進行分期,採用直線相關分析DLC-1基因錶達與結腸癌分期及淋巴結轉移的相關性.結果 結腸癌組織DLC-1基因mRNA的錶達水平(0.085±0.004)明顯低于癌徬組織(0.562±0.063),癌徬組織低于正常組織(1.000±0.003).DLC-1基因mRNA錶達水平與結腸癌分期呈負相關(r=0.51,P＜ 0.01),DLC-1基因mRNA錶達水平與淋巴結轉移呈負相關(r=0.46,P＜ 0.05).結論 DLC-1基因mRNA錶達與病理學分期、淋巴結轉移率呈負相關,DLC-1基因在結腸癌髮生、髮展中扮縯重要角色,可能是研究結腸癌新的突破口.
목적 탐토간암결실-1(DLC-1)기인mRNA표체여결장암분기급림파결전이적상관성.방법 수집2009년9월지2011년9월수술절제결장암표본공60례,이외상、결장게실、결장염수술치료류취적정상조직10례위대조.채용반전록PCR정량검측결장암조직、암방조직、정상조직DLC-1기인mRNA적표체.의거TNM분기진행분기,채용직선상관분석DLC-1기인표체여결장암분기급림파결전이적상관성.결과 결장암조직DLC-1기인mRNA적표체수평(0.085±0.004)명현저우암방조직(0.562±0.063),암방조직저우정상조직(1.000±0.003).DLC-1기인mRNA표체수평여결장암분기정부상관(r=0.51,P＜ 0.01),DLC-1기인mRNA표체수평여림파결전이정부상관(r=0.46,P＜ 0.05).결론 DLC-1기인mRNA표체여병이학분기、림파결전이솔정부상관,DLC-1기인재결장암발생、발전중분연중요각색,가능시연구결장암신적돌파구.
Objective To investigate the relationship between the expression level of deleted in liver cancer-1 (DLC-1) gene and colon cancer staging or lymph nodes metastatic ratio.Methods Total 60 resection specimens were collected from colon cancer patients between September 2009 and September 2011,and 10 resection specimens from patients with trauma,colonic diverticulitis and colitis were used as the control.The DLC-1 mRNA level was measured with real time quantitative RT-PCR in colon cancer patients and the control group.TNM system was used for colon cancer staging.The linear correlation analysis was performed to determine the relationship between DLC-1 expression level and colon cancer staging or lymph nodes metastatic ratio.Results The mRNA level of DLC-1 was significantly reduced in colon cancer tissues in comparison to that in adjacent normal tissues,while adjacent tissues showed significantly lower DLC-1 level than normal clone tissues.The expression of DLC-1 was negatively correlated with both colon cancer stage and the lymph nodes metastatic ratio (r =0.46,P ＜ 0.05).Conclusions DLC-1 expression was negatively correlated with pathological stage and lymph node metastasis ratio,indicating a critical role of DLC-1 gene in colon cancer genesis,which provide a possible new direction for colon cancer research.
