肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2014年
4期
250-252,256
,共4页
李健%郭庚%范益民%郝解贺%李瑾
李健%郭庚%範益民%郝解賀%李瑾
리건%곽경%범익민%학해하%리근
神经胶质瘤%蛋白,Dishevelled%肿瘤增殖%肿瘤侵润
神經膠質瘤%蛋白,Dishevelled%腫瘤增殖%腫瘤侵潤
신경효질류%단백,Dishevelled%종류증식%종류침윤
Glioma%Protein,dishevelled%Tumor proliferation%Neoplasm invasiveness
目的 探讨Dishevelled蛋白表达与脑胶质瘤细胞增殖、侵袭的相关性.方法 选取67例脑胶质瘤患者组织标本,采用免疫组织化学法检测Dishevelled蛋白的表达,评定Dishevelled蛋白免疫反应评分(IRS)、肿瘤增殖指数(PI)和侵袭指数(Ⅱ),并分析其相关性.结果 67例脑胶质瘤中,Dishevelled蛋白阳性率为65.7％(44/67),Dishevelled蛋白IRS及肿瘤PⅠ、Ⅱ分别为4.15±3.13、(30.93±17.92)％、(20.38±13.36)％,均随脑胶质瘤病理级别的增高而升高(P＜0.001),Dishevelled蛋白阳性组PⅠ、Ⅱ高于Dishevelled蛋白阴性组[(38.27±17.60)％比(16.02±8.92)％;(30.03±13.81)％比(10.63±4.41)％,均P＜ 0.001].肿瘤PⅠ、Ⅱ与Dishevelled蛋白IRS呈正相关(相关系数r分别为0.940、0.953).结论 Dishevelled蛋白高表达在脑胶质瘤恶性增殖、侵袭中起着重要的作用.
目的 探討Dishevelled蛋白錶達與腦膠質瘤細胞增殖、侵襲的相關性.方法 選取67例腦膠質瘤患者組織標本,採用免疫組織化學法檢測Dishevelled蛋白的錶達,評定Dishevelled蛋白免疫反應評分(IRS)、腫瘤增殖指數(PI)和侵襲指數(Ⅱ),併分析其相關性.結果 67例腦膠質瘤中,Dishevelled蛋白暘性率為65.7％(44/67),Dishevelled蛋白IRS及腫瘤PⅠ、Ⅱ分彆為4.15±3.13、(30.93±17.92)％、(20.38±13.36)％,均隨腦膠質瘤病理級彆的增高而升高(P＜0.001),Dishevelled蛋白暘性組PⅠ、Ⅱ高于Dishevelled蛋白陰性組[(38.27±17.60)％比(16.02±8.92)％;(30.03±13.81)％比(10.63±4.41)％,均P＜ 0.001].腫瘤PⅠ、Ⅱ與Dishevelled蛋白IRS呈正相關(相關繫數r分彆為0.940、0.953).結論 Dishevelled蛋白高錶達在腦膠質瘤噁性增殖、侵襲中起著重要的作用.
목적 탐토Dishevelled단백표체여뇌효질류세포증식、침습적상관성.방법 선취67례뇌효질류환자조직표본,채용면역조직화학법검측Dishevelled단백적표체,평정Dishevelled단백면역반응평분(IRS)、종류증식지수(PI)화침습지수(Ⅱ),병분석기상관성.결과 67례뇌효질류중,Dishevelled단백양성솔위65.7％(44/67),Dishevelled단백IRS급종류PⅠ、Ⅱ분별위4.15±3.13、(30.93±17.92)％、(20.38±13.36)％,균수뇌효질류병리급별적증고이승고(P＜0.001),Dishevelled단백양성조PⅠ、Ⅱ고우Dishevelled단백음성조[(38.27±17.60)％비(16.02±8.92)％;(30.03±13.81)％비(10.63±4.41)％,균P＜ 0.001].종류PⅠ、Ⅱ여Dishevelled단백IRS정정상관(상관계수r분별위0.940、0.953).결론 Dishevelled단백고표체재뇌효질류악성증식、침습중기착중요적작용.
Objective To investigate the correlation between Dishevelled protein expression and the proliferation and invasion of glioma cells.Methods 67 cases of brain glioma specimens were collected.The expression of Dishevelled protein was detected with immunohistochemical method.The immunoreactivity score (IRS) of Dishevelled protein,and proliferation index (PⅠ) and invasion index (Ⅱ) were measured and their correlations were analyzed.Results The positive rate of Dishevelled protein in glioma was 65.7 ％ (44/67).IRS,PⅠ and Ⅱ were 4.15±3.13,(30.93±17.92) ％,(20.38±13.36) ％,respectively.Both PⅠ and Ⅱ significantly increased with an increase in the pathological grade of brain glioma (P ＜ 0.001).Furthermore,PⅠ and Ⅱ were significantly higher in the Dishevelled protein-positive group than those in the Dishevelled protein-negative group [(38.27±17.60) ％ vs (16.02±8.92) ％ of PⅠ and (30.03±13.81) ％ vs (10.63±4.41) ％ of Ⅱ,respectively,P ＜ 0.001].PⅠ and Ⅱ of glioma cells were positively correlated with IRS of Dishevelled protein (r =0.940 between PⅠ and IRS,and r =0.953 between Ⅱ and IRS,respectively).Conclusion Dishevelled protein plays an important role in the proliferation and invasion of brain malignant glioma.
