CAJ | 학술논문

目的分析p73基因及其蛋白在鼻咽癌组织中的表达及其与临床病理特征的相关性.方法采用实时荧光定量PCR(RQ-RT-PCR)法检测52例鼻咽癌组织和25例正常鼻咽组织p73基因表达水平,采用免疫组织化学染色法检测p73蛋白的表达水平,并分析它们与各项临床病理指标的相关性.结果 52例鼻咽癌组织中p73基因表达阳性38例(73.1％),蛋白阳性表达37例(71.2％);鼻咽正常组织中p73基因表达阳性6例(24.0％),蛋白阳性表达9例(36.0％).p73基因及其蛋白在鼻咽癌组织中的表达均高于正常鼻咽组织,差异均有统计学意义(均P＜0.05),且p73基因及其蛋白的表达与癌组织的浸润深度、病理分化程度、临床分期密切相关(P＜0.05),与患者的年龄、性别无关(P＞0.05).结论检测p73基因及其蛋白的表达将对鼻咽癌的诊断、预后判断具有重要的临床意义.
목적 분석p73기인급기단백재비인암조직중적표체급기여림상병리특정적상관성.방법 채용실시형광정량PCR(RQ-RT-PCR)법검측52례비인암조직화25례정상비인조직p73기인표체수평,채용면역조직화학염색법검측p73단백적표체수평,병분석타문여각항림상병리지표적상관성.결과 52례비인암조직중p73기인표체양성38례(73.1％),단백양성표체37례(71.2％);비인정상조직중p73기인표체양성6례(24.0％),단백양성표체9례(36.0％).p73기인급기단백재비인암조직중적표체균고우정상비인조직,차이균유통계학의의(균P＜0.05),차p73기인급기단백적표체여암조직적침윤심도、병리분화정도、림상분기밀절상관(P＜0.05),여환자적년령、성별무관(P＞0.05).결론 검측p73기인급기단백적표체장대비인암적진단、예후판단구유중요적림상의의.
Objective To investigate the expression of p73 gene and its protein and their relation with clinicopathologic features in nasopharyngeal carcinoma (NPC) tissues.Methods Expression of p73 mRNA and protein in 52 NPC and 25 normal nasopharyngeal tissues was detected by real-time fluorescent quantitative PCR and immunohistochemistry.Results Expression of p73 mRNA and protein was significantly higher in NPC than that in normal nasopharyngeal tissues (mRNA:73.1％ vs 24.0 ％,protein:71.2 ％ vs 36.0 ％),there were significant statistical differences between the two groups (P ＜ 0.05),and their expression was closely related to tumor invasion depth,degree of differentiation and clinical stage (P ＜ 0.05).Expression of p73 gene and protein was not closely related to age and gender (P ＞ 0.05).Conclusion Detection expression of p73 mRNA and its protein can be helpful in the diagnosis and prognostic evaluation in NPC.