肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2014年
7期
446-450
,共5页
雷俊梅%李树峰%李英%彭江滨%刘维国
雷俊梅%李樹峰%李英%彭江濱%劉維國
뢰준매%리수봉%리영%팽강빈%류유국
胃肿瘤%多药耐药%DNA拓扑异构酶类,Ⅱ型%谷胱甘肽S-转移酶π
胃腫瘤%多藥耐藥%DNA拓撲異構酶類,Ⅱ型%穀胱甘肽S-轉移酶π
위종류%다약내약%DNA탁복이구매류,Ⅱ형%곡광감태S-전이매π
Stomach neoplasms%Multidrug resistance%DNA topoisomerases,type Ⅱ%Glutathione S-transferase π
目的 探讨拓扑异构酶Ⅱ(Topo-Ⅱ)、谷胱甘肽S-转移酶π(GST-π)在胃癌组织和正常胃黏膜组织中的表达情况,揭示其与临床病理特征的关系及其意义.方法 应用免疫组织化学法检测100例胃癌组织及20例正常胃黏膜中Topo-Ⅱ、GST-π的表达,结合临床病理资料进行综合分析.结果 Topo-Ⅱ在正常组织与胃癌高、中、低不同分化程度组织中阳性表达率分别为5.0％(1/20)、100 ％(30/30)、96.7％(29/30)、87.5％(35/40),GST-π阳性表达率分别为60.0％(12/20)、83.3 ％(24/30)、96.7％(29/30)、100.0％(40/40),Topo-Ⅱ、GST-π在正常组织与胃癌高、中、低不同分化程度组织中表达差异有统计学意义(均P＜ 0.05).Topo-Ⅱ、GST-π在胃癌组织中的表达与患者的性别、年龄、肿瘤部位、浸润深度均无关(均P＞ 0.05);Topo-Ⅱ与肿瘤的分化程度及淋巴结转移有关,随肿瘤分化程度的降低、淋巴结转移的增多,Topo-Ⅱ表达降低;GST-π与肿瘤直径、分化程度、淋巴结转移相关(均P＜ 0.05),分化程度越低、淋巴结转移越多、肿瘤直径越大,GST-π表达越高.GST-π与Topo-Ⅱ负相关(P＜0.01).结论 胃癌组织中,Topo-Ⅱ的表达与肿瘤的分化程度及淋巴结转移有关,GST-π与肿瘤直径、分化程度、淋巴结转移相关,二者呈负相关.
目的 探討拓撲異構酶Ⅱ(Topo-Ⅱ)、穀胱甘肽S-轉移酶π(GST-π)在胃癌組織和正常胃黏膜組織中的錶達情況,揭示其與臨床病理特徵的關繫及其意義.方法 應用免疫組織化學法檢測100例胃癌組織及20例正常胃黏膜中Topo-Ⅱ、GST-π的錶達,結閤臨床病理資料進行綜閤分析.結果 Topo-Ⅱ在正常組織與胃癌高、中、低不同分化程度組織中暘性錶達率分彆為5.0％(1/20)、100 ％(30/30)、96.7％(29/30)、87.5％(35/40),GST-π暘性錶達率分彆為60.0％(12/20)、83.3 ％(24/30)、96.7％(29/30)、100.0％(40/40),Topo-Ⅱ、GST-π在正常組織與胃癌高、中、低不同分化程度組織中錶達差異有統計學意義(均P＜ 0.05).Topo-Ⅱ、GST-π在胃癌組織中的錶達與患者的性彆、年齡、腫瘤部位、浸潤深度均無關(均P＞ 0.05);Topo-Ⅱ與腫瘤的分化程度及淋巴結轉移有關,隨腫瘤分化程度的降低、淋巴結轉移的增多,Topo-Ⅱ錶達降低;GST-π與腫瘤直徑、分化程度、淋巴結轉移相關(均P＜ 0.05),分化程度越低、淋巴結轉移越多、腫瘤直徑越大,GST-π錶達越高.GST-π與Topo-Ⅱ負相關(P＜0.01).結論 胃癌組織中,Topo-Ⅱ的錶達與腫瘤的分化程度及淋巴結轉移有關,GST-π與腫瘤直徑、分化程度、淋巴結轉移相關,二者呈負相關.
목적 탐토탁복이구매Ⅱ(Topo-Ⅱ)、곡광감태S-전이매π(GST-π)재위암조직화정상위점막조직중적표체정황,게시기여림상병리특정적관계급기의의.방법 응용면역조직화학법검측100례위암조직급20례정상위점막중Topo-Ⅱ、GST-π적표체,결합림상병리자료진행종합분석.결과 Topo-Ⅱ재정상조직여위암고、중、저불동분화정도조직중양성표체솔분별위5.0％(1/20)、100 ％(30/30)、96.7％(29/30)、87.5％(35/40),GST-π양성표체솔분별위60.0％(12/20)、83.3 ％(24/30)、96.7％(29/30)、100.0％(40/40),Topo-Ⅱ、GST-π재정상조직여위암고、중、저불동분화정도조직중표체차이유통계학의의(균P＜ 0.05).Topo-Ⅱ、GST-π재위암조직중적표체여환자적성별、년령、종류부위、침윤심도균무관(균P＞ 0.05);Topo-Ⅱ여종류적분화정도급림파결전이유관,수종류분화정도적강저、림파결전이적증다,Topo-Ⅱ표체강저;GST-π여종류직경、분화정도、림파결전이상관(균P＜ 0.05),분화정도월저、림파결전이월다、종류직경월대,GST-π표체월고.GST-π여Topo-Ⅱ부상관(P＜0.01).결론 위암조직중,Topo-Ⅱ적표체여종류적분화정도급림파결전이유관,GST-π여종류직경、분화정도、림파결전이상관,이자정부상관.
Objective To explore the expressions of Topo-Ⅱ,GST-π in gastric cancer tissues and normal gastric mucosa tissues,to reveal its relationship with clinical pathological features and significance.Methods Immunohistochemical method was used to detect Topo-Ⅱ,GST-π expressions in 100 cases of gastric carcinoma and 20 cases of normal gastric mucosa,make a comprehensive analysis combined with clinical pathology data.Results There were significant difference of expression of Topo-Ⅱ,GST-π between the normal gastric mucosa tissues and gastric cancer of different degree of differentiation.Topo-Ⅱ positive expression rate of 5.0 ％ (1/20),100 ％ (30/30),96.7 ％ (29/30) and 87.5 ％ (35/40) respectively; GST-π positive expression rate were 60.0 ％ (12/20),83.3 ％ (24/30),96.7 ％ (29/30) and 100.0 ％ (40/40) respectively (P ＜ 0.05).The expressions of Topo-Ⅱ,GST-π in gastric cancer tissue were not relevant to patient' s sex,age,tumor location,infiltration depth (P ＞ 0.05).Topo-Ⅱ associated with the differentiation degree and lymph node metastasis of tumors,with the decreasing degree of tumor differentiation,lymph node metastasis,Topo-Ⅱ expression also decreased.GST-π was associated with tumor diameter,degree of differentiation and lymph node metastasis,the lower the degree of differentiation,lymph node metastasis,tumor diameter the more,GST-π expression increased (P ＜ 0.05).GST-π and Topo-Ⅱ were negative correlation and both expressed in gastric cancer tissue (P ＜ 0.01).Conclusions The expressions of Topo-Ⅱ in gastric cancer tissue is associated with the differentiation degree and lymph node metastasis of the tumor.GST-π is associated with tumor diameter,the degree of differentiation and lymph node metastasis.GST-π and Topo-Ⅱ in gastric cancer tissues are negatively correlated.
