肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2014年
7期
454-457
,共4页
李立平%赵亚刚%吴炜景%谢子英%马艳春%周兰
李立平%趙亞剛%吳煒景%謝子英%馬豔春%週蘭
리립평%조아강%오위경%사자영%마염춘%주란
胃肿瘤%胃溃疡%电子胃镜%miR-21
胃腫瘤%胃潰瘍%電子胃鏡%miR-21
위종류%위궤양%전자위경%miR-21
Stomach neoplasms%Gastric ulcer%Electronic gastroscopy%miR-21
目的 探讨电子胃镜检查联合组织miR-21表达水平检测对胃癌诊断的意义.方法 纳入2011年6月至2012年12月进行胃镜检查的患者96例,胃镜检查同时胃镜下钳取患者胃内病变组织,通过对比胃镜诊断与病理诊断结果计算胃镜确诊率及漏诊率;应用实时荧光定量PCR对组织进行miR-21表达水平检测,对比胃镜下不同表现的各种疾病的miR-21表达水平.结果 胃癌、胃溃疡、慢性浅表性胃炎、胃食管反流性胃炎、慢性萎缩性胃炎检出率分别为28.12％(27/96)、30.21％(29/96)、18.75％(18/96)、10.42％(10/96)、12.50 ％(12/96),胃镜检查胃癌确诊率为100.00％,漏诊率6.90％(2/29);胃癌组miR-21相对表达量为54.41±6.66,明显高于胃溃疡组及其他疾病组(P＜0.01).结论 电子胃镜检查能较好地对胃癌患者进行筛查,胃镜下钳取组织行miR-21表达水平检测能提高胃镜对胃癌筛查的准确率,两者联合对胃癌诊断具重要价值.
目的 探討電子胃鏡檢查聯閤組織miR-21錶達水平檢測對胃癌診斷的意義.方法 納入2011年6月至2012年12月進行胃鏡檢查的患者96例,胃鏡檢查同時胃鏡下鉗取患者胃內病變組織,通過對比胃鏡診斷與病理診斷結果計算胃鏡確診率及漏診率;應用實時熒光定量PCR對組織進行miR-21錶達水平檢測,對比胃鏡下不同錶現的各種疾病的miR-21錶達水平.結果 胃癌、胃潰瘍、慢性淺錶性胃炎、胃食管反流性胃炎、慢性萎縮性胃炎檢齣率分彆為28.12％(27/96)、30.21％(29/96)、18.75％(18/96)、10.42％(10/96)、12.50 ％(12/96),胃鏡檢查胃癌確診率為100.00％,漏診率6.90％(2/29);胃癌組miR-21相對錶達量為54.41±6.66,明顯高于胃潰瘍組及其他疾病組(P＜0.01).結論 電子胃鏡檢查能較好地對胃癌患者進行篩查,胃鏡下鉗取組織行miR-21錶達水平檢測能提高胃鏡對胃癌篩查的準確率,兩者聯閤對胃癌診斷具重要價值.
목적 탐토전자위경검사연합조직miR-21표체수평검측대위암진단적의의.방법 납입2011년6월지2012년12월진행위경검사적환자96례,위경검사동시위경하겸취환자위내병변조직,통과대비위경진단여병리진단결과계산위경학진솔급루진솔;응용실시형광정량PCR대조직진행miR-21표체수평검측,대비위경하불동표현적각충질병적miR-21표체수평.결과 위암、위궤양、만성천표성위염、위식관반류성위염、만성위축성위염검출솔분별위28.12％(27/96)、30.21％(29/96)、18.75％(18/96)、10.42％(10/96)、12.50 ％(12/96),위경검사위암학진솔위100.00％,루진솔6.90％(2/29);위암조miR-21상대표체량위54.41±6.66,명현고우위궤양조급기타질병조(P＜0.01).결론 전자위경검사능교호지대위암환자진행사사,위경하겸취조직행miR-21표체수평검측능제고위경대위암사사적준학솔,량자연합대위암진단구중요개치.
Objective To evaluate the significance of electronic gastroscopy examination combined with miR-21 in the diagnosis of gastric carcinoma.Methods 96 gastroscopy cases in Guangzhou General Hospital of Guangzhou Military Command were adopted from June,2011 to December,2012.The patients received gastric endoscopy examination,and the diseased tissue samples were taken out at the same time.To calculate the diagnosis rate and missed rate with gastroscopy,by comparing the gastroscopy diagnosis with the pathology diagnosis.To evaluate the expression level of miR-21 of the tissue by applying quantitative real time PCR.Results The diagnosis rates of gastric cancer,gastric ulcer,chronic superficial gastritis,gastroesophageal reflux gastritis,chronic atrophic gastritis under gastroscopy were 28.12 ％ (27/96),30.21％ (29/96),18.75 ％ (18/96),10.42 ％ (10/96),12.50 ％ (12/96).The diagnosis rate of gastric cancer was 100.00 ％ and the missed diagnosis rate was 6.90 ％ (2/29),the relative transcript level of miR-21 in the gastric cancer group was 54.41±6.66,which was obviously higher than the gastric ulcer group and the other groups (P ＜ 0.01).Conclusions Electronic gastroscopy is a clinically practical examination method in screening gastric cancer cases,evaluating the expression level of miR-21 in the diseased tissue under gastroscopy can enhance the accuracy of screening gastric cancer.Combining the two methods is useful in the diagnosis of gastric cancer.
