肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2014年
8期
510-513
,共4页
乳腺肿瘤%4T1细胞株%巨噬细胞集落刺激因子%粒-巨噬细胞集落刺激因子%血管内皮生长因子A%细胞迁移
乳腺腫瘤%4T1細胞株%巨噬細胞集落刺激因子%粒-巨噬細胞集落刺激因子%血管內皮生長因子A%細胞遷移
유선종류%4T1세포주%거서세포집락자격인자%립-거서세포집락자격인자%혈관내피생장인자A%세포천이
Breast neoplasms%4T1 cell line%M-CSF%GM-CSF%VEGF-A%Cell migration
目的 探讨巨噬细胞集落刺激因子(M-CSF)和粒细胞-巨噬细胞集落刺激因子(GM-CSF)对乳腺癌4T1细胞迁移及血管内皮生长因子(VEGF)-A表达的影响.方法 分别用5、10 ng/ml M-CSF和GM-CSF处理小鼠乳腺癌4T1细胞株,采用实时荧光定量PCR方法检测4T1细胞中细胞因子VEGF-AmRNA表达量的变化.划痕实验和Transwell实验检测用5 ng/ml M-CSF、5 ng/ml GM-CSF和10 ng/mlVEGF-A对4T1细胞迁移和侵袭能力的影响.结果 经5、10ng/ml M-CSF分别处理4T1细胞后VEGF-AmRNA在12h和24 h的相对表达量分别为17.81±2.49和17.48±5.43、5.15±2.59和5.45±4.28;经5、10 ng/ml GM-CSF分别处理4T1细胞后VEGF-A mRNA在12h和24 h的相对表达量分别为9.77±2.39和7.61±2.80、6.53±2.41和6.30±2.89.与无细胞因子处理的对照组相比,除10 ng/ml GM-CSF处理4T1细胞24 h组VEGF-A mRNA表达水平差异无统计学意义(P＞0.05)外,其他各组VEGF-A mRNA表达水平差异均有统计学意义(均P＜ 0.05),处理细胞12h时VEGF-A的表达较24 h时更明显(均P＜ 0.01).划痕实验显示M-CSF和GM-CSF可促进4T1细胞的迁移,但VEGF-A对4T1细胞的迁移无影响.Transwell实验发现,用M-CSF、GM-CSF和VEGF-A处理的实验组中4T1细胞的穿膜数量多于无细胞因子处理的对照组(均P＜ 0.05).结论 M-CSF和GM-CSF可促进小鼠乳腺癌4T1细胞迁移及VEGF-A的表达.
目的 探討巨噬細胞集落刺激因子(M-CSF)和粒細胞-巨噬細胞集落刺激因子(GM-CSF)對乳腺癌4T1細胞遷移及血管內皮生長因子(VEGF)-A錶達的影響.方法 分彆用5、10 ng/ml M-CSF和GM-CSF處理小鼠乳腺癌4T1細胞株,採用實時熒光定量PCR方法檢測4T1細胞中細胞因子VEGF-AmRNA錶達量的變化.劃痕實驗和Transwell實驗檢測用5 ng/ml M-CSF、5 ng/ml GM-CSF和10 ng/mlVEGF-A對4T1細胞遷移和侵襲能力的影響.結果 經5、10ng/ml M-CSF分彆處理4T1細胞後VEGF-AmRNA在12h和24 h的相對錶達量分彆為17.81±2.49和17.48±5.43、5.15±2.59和5.45±4.28;經5、10 ng/ml GM-CSF分彆處理4T1細胞後VEGF-A mRNA在12h和24 h的相對錶達量分彆為9.77±2.39和7.61±2.80、6.53±2.41和6.30±2.89.與無細胞因子處理的對照組相比,除10 ng/ml GM-CSF處理4T1細胞24 h組VEGF-A mRNA錶達水平差異無統計學意義(P＞0.05)外,其他各組VEGF-A mRNA錶達水平差異均有統計學意義(均P＜ 0.05),處理細胞12h時VEGF-A的錶達較24 h時更明顯(均P＜ 0.01).劃痕實驗顯示M-CSF和GM-CSF可促進4T1細胞的遷移,但VEGF-A對4T1細胞的遷移無影響.Transwell實驗髮現,用M-CSF、GM-CSF和VEGF-A處理的實驗組中4T1細胞的穿膜數量多于無細胞因子處理的對照組(均P＜ 0.05).結論 M-CSF和GM-CSF可促進小鼠乳腺癌4T1細胞遷移及VEGF-A的錶達.
목적 탐토거서세포집락자격인자(M-CSF)화립세포-거서세포집락자격인자(GM-CSF)대유선암4T1세포천이급혈관내피생장인자(VEGF)-A표체적영향.방법 분별용5、10 ng/ml M-CSF화GM-CSF처리소서유선암4T1세포주,채용실시형광정량PCR방법검측4T1세포중세포인자VEGF-AmRNA표체량적변화.화흔실험화Transwell실험검측용5 ng/ml M-CSF、5 ng/ml GM-CSF화10 ng/mlVEGF-A대4T1세포천이화침습능력적영향.결과 경5、10ng/ml M-CSF분별처리4T1세포후VEGF-AmRNA재12h화24 h적상대표체량분별위17.81±2.49화17.48±5.43、5.15±2.59화5.45±4.28;경5、10 ng/ml GM-CSF분별처리4T1세포후VEGF-A mRNA재12h화24 h적상대표체량분별위9.77±2.39화7.61±2.80、6.53±2.41화6.30±2.89.여무세포인자처리적대조조상비,제10 ng/ml GM-CSF처리4T1세포24 h조VEGF-A mRNA표체수평차이무통계학의의(P＞0.05)외,기타각조VEGF-A mRNA표체수평차이균유통계학의의(균P＜ 0.05),처리세포12h시VEGF-A적표체교24 h시경명현(균P＜ 0.01).화흔실험현시M-CSF화GM-CSF가촉진4T1세포적천이,단VEGF-A대4T1세포적천이무영향.Transwell실험발현,용M-CSF、GM-CSF화VEGF-A처리적실험조중4T1세포적천막수량다우무세포인자처리적대조조(균P＜ 0.05).결론 M-CSF화GM-CSF가촉진소서유선암4T1세포천이급VEGF-A적표체.
Objective To investigate the effect of M-CSF and GM-CSF on migration and expression of VEGF-A in breast cancer cell line 4T1.Methods Real-time PCR was used to detect VEGF-A mRNA expression in 4T1 cells treated by 5 ng/ml or 10 ng/ml M-CSF or GM-CSF.Ability of migration and metastasis of 4T1 cells were analyzed by scratch and Transwell assays.Results The relative expression of VEGF-A mRNA at 12 h and 24 h in 4T1 cells treated by 5 ng/ml or 10 ng/ml M-CSF were 17.81±2.49 and 17.48± 5.43,5.15±2.59 and 5.45±4.28,respectively,while those treated by GM-CSF were 9.77±2.39 and 7.61±2.80,6.53±2.41 and 6.30±2.89,respectively.M-CSF and GM-CSF can promote the expression of VEGF-A in 4T1 cells (P ＜ 0.05).The relative expression of VEGF-A was higher in 4T1 cells treated for 12 h than that for 24 h (P ＜ 0.01).M-CSF,GM-CSF and VEGF-A can promote metastasis of 4T1 cells (all P ＜ 0.05),whereas no gross migration of 4T1 cells was showed by VEGF-A treatment.Conclusion M-CSF and GM-CSF can promote the migration and expression of VEGF-A in breast cancer cell line 4T1.
