CAJ | 학술논문

目的比较兔肝VX2移植瘤经介入性热化疗(ITC)治疗前后1H-MRS主要代谢物的变化,并与细胞凋亡指数(AI)进行相关性分析.方法选取新西兰大白兔20只,采用组织块种植的方法成功制成兔VX2肝癌模型,10d后,待肿瘤直径≥10 mm时,分别于治疗前1天、治疗后第14天行常规磁共振成像(MRI)及磁共振波谱(MRS)扫描.使用Philips 1.5T MR成像仪,选取病灶不同感兴趣区(ROI),分别测量治疗前、后不同ROI的观察肿瘤治疗前后的胆碱(Cho)峰值与脂质(Lip)峰值及两者比值的变化,免疫组织化学法及HE染色检测肿瘤细胞AI,并与MRS结果进行对照,分析不同ROI的主要代谢物变化规律.结果在成功获得的1H-MRS波谱图中多数可见到6个主要的代谢物波峰,治疗前Cho峰、Lip峰均升高;治疗后Cho峰值较治疗前下降、Lip峰值则进一步升高,Cho/Lip比值降低,两两比较差异均有统计学意义(P＜0.05);治疗后Cho/Lip峰值比与AI值呈负相关(r=-0.56,P=0.02).结论兔肝VX2移植瘤不同ROI的1H-MRS主要代谢物的变化情况与肿瘤细胞AI表达程度及范围一致;1H-MRS能反映VX2移植瘤ITC治疗前后肿瘤细胞凋亡和能量代谢变化情况.
목적 비교토간VX2이식류경개입성열화료(ITC)치료전후1H-MRS주요대사물적변화,병여세포조망지수(AI)진행상관성분석.방법 선취신서란대백토20지,채용조직괴충식적방법성공제성토VX2간암모형,10d후,대종류직경≥10 mm시,분별우치료전1천、치료후제14천행상규자공진성상(MRI)급자공진파보(MRS)소묘.사용Philips 1.5T MR성상의,선취병조불동감흥취구(ROI),분별측량치료전、후불동ROI적관찰종류치료전후적담감(Cho)봉치여지질(Lip)봉치급량자비치적변화,면역조직화학법급HE염색검측종류세포AI,병여MRS결과진행대조,분석불동ROI적주요대사물변화규률.결과 재성공획득적1H-MRS파보도중다수가견도6개주요적대사물파봉,치료전Cho봉、Lip봉균승고;치료후Cho봉치교치료전하강、Lip봉치칙진일보승고,Cho/Lip비치강저,량량비교차이균유통계학의의(P＜0.05);치료후Cho/Lip봉치비여AI치정부상관(r=-0.56,P=0.02).결론 토간VX2이식류불동ROI적1H-MRS주요대사물적변화정황여종류세포AI표체정도급범위일치;1H-MRS능반영VX2이식류ITC치료전후종류세포조망화능량대사변화정황.
Objective To compare magnetic resonance spectroscopy (MRS) characteristics and changes before and after 5-Fu interventional thermochemotherapy (ITC) as well as to analyze the correlation of Cho/Lip and apoptosis index (AI) in hepatic VX2 carcinoma animal model.Methods 20 rabbit models of hepatic VX2 carcinoma were implanted successfully.Ten days later,as tumor diameter≥ 10 mm,routine scan and 1H-MRS were performed before one day and after 14 days on all of rabbits by Philips 1.5T MR scanner.The changes of Cho peak,Lip peak and the ratio of Cho/Lip before and after the treatment were analyzed.Specimens of AI were examined and compared with the ratio of Cho/Lip.Results The Cho peak and Lip peak were increased before ITC.After ITC,the Cho peak and the ratio of Cho/Lip decreased than before.The Lip peak was further increased than that of before.AI was correlated negatively with the ratio of Cho/Lip (r =-0.56,P =0.02).Conclusions The metabolites of different ROI are matched with the levels and scopes of AI.1H-MRS can be used to evaluate tumor cell apoptosis and energy metabolism changes of the rabbit models of hepatic VX2 carcinoma with the treatment.