CAJ | 학술논문

目的探讨紧密连接蛋白CLDN1和CLDN4在人肝细胞肝癌(HCC)组织中的表达及其意义.方法收集郑州大学第一附属医院2010年至2011年手术切除的HCC组织标本60例和正常肝组织50例,采用免疫组织化学二步法分别检测CLDN1和CLDN4的表达.结果 CLDN1和CLDN4在HCC组织中的阳性表达率分别为70％(42/60)和25％ (15/60),两者的表达与患者年龄、性别、血液甲胎蛋白(AFP)浓度无关(P＞0.05),与肿瘤分化程度无关(P＞0.05).CLDN1和CLDN4在HCC中的表达具有相关性(r=0.533,P＜0.05).CLDN1在正常肝组织中无表达(0/50),CLDN4在正常肝组织中高表达(50/50).两者在正常肝组织中的表达差异有统计学意义(P＜0.05).结论肝细胞肝癌中存在CLDN1和CLDN4的异常表达,CLDN1在HCC中表达上调,而CLDN4在HCC中表达下调,两者表达具有相关性(r=0.533,P＜0.05).推测CLDN1表达上调和CLDN4表达下调能够促进肝细胞肝癌的发生、发展.
목적 탐토긴밀련접단백CLDN1화CLDN4재인간세포간암(HCC)조직중적표체급기의의.방법 수집정주대학제일부속의원2010년지2011년수술절제적HCC조직표본60례화정상간조직50례,채용면역조직화학이보법분별검측CLDN1화CLDN4적표체.결과 CLDN1화CLDN4재HCC조직중적양성표체솔분별위70％(42/60)화25％ (15/60),량자적표체여환자년령、성별、혈액갑태단백(AFP)농도무관(P＞0.05),여종류분화정도무관(P＞0.05).CLDN1화CLDN4재HCC중적표체구유상관성(r=0.533,P＜0.05).CLDN1재정상간조직중무표체(0/50),CLDN4재정상간조직중고표체(50/50).량자재정상간조직중적표체차이유통계학의의(P＜0.05).결론 간세포간암중존재CLDN1화CLDN4적이상표체,CLDN1재HCC중표체상조,이CLDN4재HCC중표체하조,량자표체구유상관성(r=0.533,P＜0.05).추측CLDN1표체상조화CLDN4표체하조능구촉진간세포간암적발생、발전.
Objective To investigate the expression and clinical significances of CLDN1 and CLDN4 in human hepatocellular carcinoma.Methods Immunohistochemical method was used to detect the expression of CLDN1 and CLDN4 in both hepatocellular carcinoma tissues(60 cases) and normal liver tissues (50 cases)and the relation between CLDN1 and CLDN4 was evaluated.Results The positive rates of CLDN1 and CLDN4 expressions in HCC tissues were 70％ (42/60) and 25％ (15/60) respectively.The positive rates of CLDN1 and CLDN4 expressions in normal liver were 0 (0/50) and 100％ (50/50).The expressions of CLDN1 and CLDN4 had no relationship with age,sex,AFP and degree of tumor differentiation of the patients with HCC.The expression of CLDN4 was correlated with the expression of CLDN1 in HCC.Conclusions The expressions of CLDN1 and CLDN4 in HCC are both abnormal.The expressions ofCLDN4 in HCC are down-regulated and the expressions of CLDN1 in HCC are over-expressed.Abnormal expressions of CLDN1 and CLDN4 in HCC may promote the development and progression of HCC.