CAJ | 학술논문

目的研究急性白血病(AL)患儿外周血T淋巴细胞亚群及血清转化生长因子-β含量的变化及临床意义.方法采用免疫荧光流式细胞技术检测45例AL患儿及45例正常儿童外周血T淋巴细胞亚群CD3+T细胞、CD4+T细胞及CD8+T细胞水平及自然杀伤(NK)细胞活性；采用ELISA方法检测上述AL患儿及正常儿童血清TGF-β含量.结果 AL患儿CD3+T细胞、CD4+T细胞及CD8+T细胞百分比均低于正常儿童(P＜0.01)；AL患儿NK细胞活性(6±4)明显低于正常儿童(13±5),差异有统计学意义(P＜0.01)；而AL患儿血清TGF-β含量(3.9±1.3)μg/L明显低于正常儿童(8.1±2.7) μg/L,差异有统计学意义(P＜0.01).结论 AL患儿T淋巴细胞亚群及NK细胞比例改变,提示AL患儿存在免疫功能异常,而血清TGF-β含量与AL患儿免疫功能密切相关,为进一步揭示AL发病机制提供理论依据.
목적 연구급성백혈병(AL)환인외주혈T림파세포아군급혈청전화생장인자-β함량적변화급림상의의.방법 채용면역형광류식세포기술검측45례AL환인급45례정상인동외주혈T림파세포아군CD3+T세포、CD4+T세포급CD8+T세포수평급자연살상(NK)세포활성；채용ELISA방법검측상술AL환인급정상인동혈청TGF-β함량.결과 AL환인CD3+T세포、CD4+T세포급CD8+T세포백분비균저우정상인동(P＜0.01)；AL환인NK세포활성(6±4)명현저우정상인동(13±5),차이유통계학의의(P＜0.01)；이AL환인혈청TGF-β함량(3.9±1.3)μg/L명현저우정상인동(8.1±2.7) μg/L,차이유통계학의의(P＜0.01).결론 AL환인T림파세포아군급NK세포비례개변,제시AL환인존재면역공능이상,이혈청TGF-β함량여AL환인면역공능밀절상관,위진일보게시AL발병궤제제공이론의거.
Objective To study the changes of T lymphocyte subtype levels and the contents of serum transforming growth factor β (TGF-β) in children with acute leukemia (AL).Methods The Flow Cytometry (FCM) used to detected the T lymphocyte subtype and NK cells in 45 cases of AL.ELISA used to detected the changes of TGF-β in AL patients and control group.Results The study showed that NK cells were greatly decreased in comprison with normal controls (NC) (P ＜ 0.01),CD3 + cells and CD4+ cells decreased in comparison with NC (P ＜ 0.01) and TGF-β levels increased in AL patients.Conclusions Tlymphocyte subtype and NK cells can evaluate the immune function of children with acute leukemia,TGF-β has intimate relationship with immune function and provide experimental basis for clinic immunotherapy of AL patients.