中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2012年
23期
36-38
,共3页
呼琳%刘琦%谢娟珂%宋小兵%韦多%殷宝莉%张翠莲
呼琳%劉琦%謝娟珂%宋小兵%韋多%慇寶莉%張翠蓮
호림%류기%사연가%송소병%위다%은보리%장취련
程序化冷冻%玻璃化冷冻%卵裂球损伤%胚胎移植
程序化冷凍%玻璃化冷凍%卵裂毬損傷%胚胎移植
정서화냉동%파리화냉동%란렬구손상%배태이식
Programmed-freezing%Vitrification%Blastomere damage%Embryo transfer
目的 探讨玻璃化冷冻法和程序化冷冻法与复苏后卵裂球损伤胚胎的移植周期临床结局的关系.方法 回顾性分析257个玻璃化冷冻周期和152个程序化冷冻周期,均解冻2枚胚胎并移植,根据移植胚胎卵裂球损伤情况分为完全存活组(A组)、1枚胚胎卵裂球损伤组(B组)、2枚胚胎卵裂球均损伤组(C组),比较两种冷冻方法复苏后胚胎完全存活率,以及卵裂球损伤胚胎移植周期的妊娠率、种植率、早期流产率等指标.结果 在均解冻并移植2枚胚胎的情况下,玻璃化冷冻法组复苏后胚胎完全存活率显著高于程序化冷冻法组(78.79％ vs40.46％,P＜0.01),但在移植有1枚胚胎卵裂球损伤组妊娠率(47.61％ vs 54.39％,P＞0.05)、种植率(29.36％vs 38.60％,P＞0.05)、早期流产率(20.00％ vs 16.13％,P＞0.05)比较差异无统计学意义；移植有2枚胚胎卵裂球均损伤组妊娠率(30.43％ vs 33.87％,P＞0.05)、种植率(19.56％ vs 23.39％,P＞0.05)、早期流产率(28.57％ vs 9.52％,P＞0.05)比较差异无统计学意义.结论 玻璃化冷冻法与程序化冷冻法相比,能显著提高胚胎复苏的完全存活率,但对复苏后有卵裂球损伤胚胎的移植周期临床结局没有影响.
目的 探討玻璃化冷凍法和程序化冷凍法與複囌後卵裂毬損傷胚胎的移植週期臨床結跼的關繫.方法 迴顧性分析257箇玻璃化冷凍週期和152箇程序化冷凍週期,均解凍2枚胚胎併移植,根據移植胚胎卵裂毬損傷情況分為完全存活組(A組)、1枚胚胎卵裂毬損傷組(B組)、2枚胚胎卵裂毬均損傷組(C組),比較兩種冷凍方法複囌後胚胎完全存活率,以及卵裂毬損傷胚胎移植週期的妊娠率、種植率、早期流產率等指標.結果 在均解凍併移植2枚胚胎的情況下,玻璃化冷凍法組複囌後胚胎完全存活率顯著高于程序化冷凍法組(78.79％ vs40.46％,P＜0.01),但在移植有1枚胚胎卵裂毬損傷組妊娠率(47.61％ vs 54.39％,P＞0.05)、種植率(29.36％vs 38.60％,P＞0.05)、早期流產率(20.00％ vs 16.13％,P＞0.05)比較差異無統計學意義；移植有2枚胚胎卵裂毬均損傷組妊娠率(30.43％ vs 33.87％,P＞0.05)、種植率(19.56％ vs 23.39％,P＞0.05)、早期流產率(28.57％ vs 9.52％,P＞0.05)比較差異無統計學意義.結論 玻璃化冷凍法與程序化冷凍法相比,能顯著提高胚胎複囌的完全存活率,但對複囌後有卵裂毬損傷胚胎的移植週期臨床結跼沒有影響.
목적 탐토파리화냉동법화정서화냉동법여복소후란렬구손상배태적이식주기림상결국적관계.방법 회고성분석257개파리화냉동주기화152개정서화냉동주기,균해동2매배태병이식,근거이식배태란렬구손상정황분위완전존활조(A조)、1매배태란렬구손상조(B조)、2매배태란렬구균손상조(C조),비교량충냉동방법복소후배태완전존활솔,이급란렬구손상배태이식주기적임신솔、충식솔、조기유산솔등지표.결과 재균해동병이식2매배태적정황하,파리화냉동법조복소후배태완전존활솔현저고우정서화냉동법조(78.79％ vs40.46％,P＜0.01),단재이식유1매배태란렬구손상조임신솔(47.61％ vs 54.39％,P＞0.05)、충식솔(29.36％vs 38.60％,P＞0.05)、조기유산솔(20.00％ vs 16.13％,P＞0.05)비교차이무통계학의의；이식유2매배태란렬구균손상조임신솔(30.43％ vs 33.87％,P＞0.05)、충식솔(19.56％ vs 23.39％,P＞0.05)、조기유산솔(28.57％ vs 9.52％,P＞0.05)비교차이무통계학의의.결론 파리화냉동법여정서화냉동법상비,능현저제고배태복소적완전존활솔,단대복소후유란렬구손상배태적이식주기림상결국몰유영향.
Objective To compare the clinical outcome of different freezing methods (vitrification and programmed-freezing) and survival state of blastomere cells after frozen-thawed.Methods A retrospective study was performed by analyzing 257 cycles and 152 cycles,which freezing embryos with vitrification and programmed-freezing.Both numbers of embryos thawed and transferred of all cycles were 2 embryos.According to the survival state of blatomeres after frozen-thawed,both groups vitrification and programmed-freezing were divided into 3 subgroups:subgroup A,both embryos were intact; subgroup B,one embryo was damaged;subgroup C,both embryos were damaged.Intact embryos rate,pregnancy rate,implantation rate,early abortion rate was compared.Results The difference in intact embryo rate between programmed-freezing and vitrification was significantly(78.79％ vs 40.46％,P ＜ 0.01).There were no statistically significant in the pregnancy rate(47.61％ vs 54.39％,P ＞ 0.05) (30.43％ vs 33.87％,P ＞ 0.05),implantation rate(29.36％ vs 38.60％,P ＞ 0.05) (19.56％ vs 23.39％,P ＞ 0.05) and early abortion rate(20.00％ vs 16.13％,P ＞ 0.05)(28.57％ vs 9.52％,P ＞ 0.05) of subgroup B and C between the two freezing methods.Conclusions Compare to programmed-freezing,vitrification is a method that could improve intact embryos rate after frozen-thawed.However,there is no significant difference in outcomes of blastomere damaged embryos transferred cycles.
