中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2013年
2期
27-29
,共3页
重组蛋白MPT63%酶联免疫吸附试验%ROC曲线%cut-off值
重組蛋白MPT63%酶聯免疫吸附試驗%ROC麯線%cut-off值
중조단백MPT63%매련면역흡부시험%ROC곡선%cut-off치
Recombination protein MPT63%ELISA%ROC%Cut-off
目的 建立规范的结核分枝杆菌(MTB)特异性抗原重组蛋白MPT63酶联免疫吸附试验(ELISA)检测系统.方法 采用方阵法确定蛋白包被浓度和酶标二抗浓度,以ROC曲线法确定MPT63蛋白ELISA法检测的cut-off值.探讨规范的ELISA检测系统建立的方法.结果 ELISA检测系统MPT63蛋白包被浓度1:128 000,酶浓度1∶1000时为最佳；在cut-off值为0.35 ～0.40时检测MPT63蛋白抗体的灵敏度和特异性最为理想,分别是75.0％和78.6％.结论 用方阵法确定蛋白包被浓度和酶标二抗浓度,并以ROC曲线法确立cut-off值是建立规范化的ELISA检测系统的关键环节.
目的 建立規範的結覈分枝桿菌(MTB)特異性抗原重組蛋白MPT63酶聯免疫吸附試驗(ELISA)檢測繫統.方法 採用方陣法確定蛋白包被濃度和酶標二抗濃度,以ROC麯線法確定MPT63蛋白ELISA法檢測的cut-off值.探討規範的ELISA檢測繫統建立的方法.結果 ELISA檢測繫統MPT63蛋白包被濃度1:128 000,酶濃度1∶1000時為最佳；在cut-off值為0.35 ～0.40時檢測MPT63蛋白抗體的靈敏度和特異性最為理想,分彆是75.0％和78.6％.結論 用方陣法確定蛋白包被濃度和酶標二抗濃度,併以ROC麯線法確立cut-off值是建立規範化的ELISA檢測繫統的關鍵環節.
목적 건립규범적결핵분지간균(MTB)특이성항원중조단백MPT63매련면역흡부시험(ELISA)검측계통.방법 채용방진법학정단백포피농도화매표이항농도,이ROC곡선법학정MPT63단백ELISA법검측적cut-off치.탐토규범적ELISA검측계통건립적방법.결과 ELISA검측계통MPT63단백포피농도1:128 000,매농도1∶1000시위최가；재cut-off치위0.35 ～0.40시검측MPT63단백항체적령민도화특이성최위이상,분별시75.0％화78.6％.결론 용방진법학정단백포피농도화매표이항농도,병이ROC곡선법학립cut-off치시건립규범화적ELISA검측계통적관건배절.
Objective To set up a standard testing system of mycobacterium tuberculosis specific antigen-recombinated MPT63 protein by enzyme-linked immunosorbent assay method.Methods The square matrix method was used to determine the concentration of coated protein and the concentration of enzyme labeling antibody.Receiver operating characteristic method was used to determine the cut-off value of ELISA detected MPT63 protein.To approach an establishment of standard ELISA detection system.Results ELISA detected MPT63 protein may attain higher sensibility and specificity when the coated protein was diluted according to 1 ∶ 128 000 and the enzyme labeling antibody was diluted by 1 ∶ 1000.Conclusions The critical elements in ELISA detection system are the application of square matrix method to determine the concentration of coated protein and the concentration of enzyme labeling antibody and application the ROC to determine cut-off.
