CAJ | 학술논문

目的研究人肺癌组织中MALAT-1 lncRNA的表达和肺癌临床病理特征之间的关系.方法采用实时荧光定量PCR技术对76例非小细胞肺癌患者的肺癌组织和对应的正常肺组织的MALAT-1的表达水平进行检测.结果 58％ (44/76)肺癌患者的癌组织中MALAT-1表达水平显著低于其对应的正常肺组织中的水平(t =-7.648,P＜0.001).肺癌组织中的MALAT-1的表达水平与吸烟史、细胞分化程度、组织学类型以及术后转移有关(P＜0.05).MALAT-1表达量降低＜50％组的患者生存期明显长于MALAT-1表达量降低≥50％组；Cox多因素回归分析显示细胞分化程度,转移,病理类型和MALAT-1表达水平是影响本组患者预后的独立因素.结论 MALAT-1将来可能作为非小细胞肺癌预后判断的分子标志物.
목적 연구인폐암조직중MALAT-1 lncRNA적표체화폐암림상병리특정지간적관계.방법 채용실시형광정량PCR기술대76례비소세포폐암환자적폐암조직화대응적정상폐조직적MALAT-1적표체수평진행검측.결과 58％ (44/76)폐암환자적암조직중MALAT-1표체수평현저저우기대응적정상폐조직중적수평(t =-7.648,P＜0.001).폐암조직중적MALAT-1적표체수평여흡연사、세포분화정도、조직학류형이급술후전이유관(P＜0.05).MALAT-1표체량강저＜50％조적환자생존기명현장우MALAT-1표체량강저≥50％조；Cox다인소회귀분석현시세포분화정도,전이,병리류형화MALAT-1표체수평시영향본조환자예후적독립인소.결론 MALAT-1장래가능작위비소세포폐암예후판단적분자표지물.
Objective To study the relationship between lung cancer clinicopathological features and MALAT-1 lncRNA expression in human lung cancer tissues.Methods The MALAT-1 expression levels in the cancerous and normal tissues of 76 lung cancer patients were measured by real-time quantitative PCR (RT-qPCR).Results The expression levels of MALAT-1 gene were down-regulated in 58％ (44/76) primary lung cancer patients,compared with the matched normal tissues.The difference of MALAT-1 expression level between cancerous tissues and the matched normal tissues was significant (t =-7.648,P ＜0.001).Furthermore,The expression levels of MALAT-1 had difference among cell differentiation,tumor metastasis,tumor pathological classification and smoking (P ＜ 0.05).The survival time of the group which had ＜ 50％ decreased MALAT-1 expression level was significantly longer than that whose MALAT-1 expression level were reduced ≥50％.Cox multivariate regression analysis showed that cell differentiation,tumor metastasis,tumor pathological classification amd expression of MALAT-1 were an independent prognostic factor for NSCLC.Conclusions The expression of MALAT-1 might play an important role in non-small lung cancer and thus maybe a biomarker for lung cancer in screening,diagnosis and prognosis.