中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2013年
14期
24-25
,共2页
不规则抗体%微柱凝胶%聚凝胺%对照
不規則抗體%微柱凝膠%聚凝胺%對照
불규칙항체%미주응효%취응알%대조
Irregular antibody%Micro-column gel%Polybrene%Comparision
目的 比较微柱凝集和聚凝胺法的灵敏度和重复性,为不规则抗体的检测更新方法.方法 使用微柱凝集和聚凝胺法检测不规则抗体,对比观察两种方法检出的阴、阳性结果和重复检验的准确性,从而选择灵敏度高和重复性好的方法满足临床输血需要.结果 采用微柱凝胶检测不规则抗体阳性率为0.82％,采用聚凝胺检测的阳性率为0.18％,两组比较差异有统计学意义(P＜0.01).不规则抗体Rh系统为7例,MNSs系统1例,自身抗体1例.微柱凝集(9/9)和聚凝胺(2/2)均为100％,两种方法比较差异无统计学意义(P＞0.05).结论 用微柱凝集检测不规则抗体能显著提高抗体的检出率,可避免漏检而降低输血反应的发生率.
目的 比較微柱凝集和聚凝胺法的靈敏度和重複性,為不規則抗體的檢測更新方法.方法 使用微柱凝集和聚凝胺法檢測不規則抗體,對比觀察兩種方法檢齣的陰、暘性結果和重複檢驗的準確性,從而選擇靈敏度高和重複性好的方法滿足臨床輸血需要.結果 採用微柱凝膠檢測不規則抗體暘性率為0.82％,採用聚凝胺檢測的暘性率為0.18％,兩組比較差異有統計學意義(P＜0.01).不規則抗體Rh繫統為7例,MNSs繫統1例,自身抗體1例.微柱凝集(9/9)和聚凝胺(2/2)均為100％,兩種方法比較差異無統計學意義(P＞0.05).結論 用微柱凝集檢測不規則抗體能顯著提高抗體的檢齣率,可避免漏檢而降低輸血反應的髮生率.
목적 비교미주응집화취응알법적령민도화중복성,위불규칙항체적검측경신방법.방법 사용미주응집화취응알법검측불규칙항체,대비관찰량충방법검출적음、양성결과화중복검험적준학성,종이선택령민도고화중복성호적방법만족림상수혈수요.결과 채용미주응효검측불규칙항체양성솔위0.82％,채용취응알검측적양성솔위0.18％,량조비교차이유통계학의의(P＜0.01).불규칙항체Rh계통위7례,MNSs계통1례,자신항체1례.미주응집(9/9)화취응알(2/2)균위100％,량충방법비교차이무통계학의의(P＞0.05).결론 용미주응집검측불규칙항체능현저제고항체적검출솔,가피면루검이강저수혈반응적발생솔.
Objective To compare the sensitivity and repeatability of micro-column gel test and polybrene test,update method for the detection of irregular antibodies.Methods Micro-column gel test and polybrene test were used for detecting irregular antibody,the results of positive and negative,the accuracy of repeated detection were compared and observed by two methods,so the high sensitivity and good reproducibility method was selected to meet the need of clinical blood transfusion.Results Irregular antibody positive rates was 0.82％,which was screened by micro-column gel test.Irregular antibody positive rates was 0.18％,which was screened by polybrene test.There was significant difference between the two groups (P ＜ 0.05).The specificity of 9 cases of irregular antibodies was as follows:seven cases were Rh,1 case was autoantibodies and the other 1 was MNSs.Conclusions Microcolumn gel test can significantly increase the detection rate and decrease the false negative results of testing,which can be helpful to reduce the incidence of transfusion reaction.
