CAJ | 학술논문

目的比较乳胶增强散射比浊法与高效液相色谱法测定血中同型半胱氨酸时的准确性、精密度、偏离度及测定范围.方法分别采用乳胶增强散射比浊法与高效液相色谱法检测70例患者和52例健康体检者血清中同型半胱氨酸,并比较两种方法测定血中同型半胱氨酸时的准确性、精密度、偏离度及测定范围.结果两法准确性、精密度均良好,各浓度值测定结果的预期偏差均在可接受范围,乳胶增强散射比浊法测定结果的相关回归方程为Y=1.336X+0.3906(r 2=0.9995),高效液相色谱法测定结果的相关回归方程为Y=0.021X-0.0085(r2=0.9999).结论乳胶增强散射比浊法是一种较好的同型半胱氨酸定量方法,值得推广.
목적 비교유효증강산사비탁법여고효액상색보법측정혈중동형반광안산시적준학성、정밀도、편리도급측정범위.방법 분별채용유효증강산사비탁법여고효액상색보법검측70례환자화52례건강체검자혈청중동형반광안산,병비교량충방법측정혈중동형반광안산시적준학성、정밀도、편리도급측정범위.결과 량법준학성、정밀도균량호,각농도치측정결과적예기편차균재가접수범위,유효증강산사비탁법측정결과적상관회귀방정위Y=1.336X+0.3906(r 2=0.9995),고효액상색보법측정결과적상관회귀방정위Y=0.021X-0.0085(r2=0.9999).결론 유효증강산사비탁법시일충교호적동형반광안산정량방법,치득추엄.
Objective To compare the accuracy,precision,deviation and metered scope between high performance liquid chromatography and nephelometry assay with latex in the determination of homocysteine.Methods Using high performance liquid chromatography and nephelometry assay with latex,70 patients and 52 healthy people fresh serum samples were measured to compare the accuracy,precision,deviation and metered scope.Results The accuracy and precision of two methods in determination of homocysteine were good.The expected deviation of both methods was in acceptable range.The regression equation of homocysteine measured by nephelometry assay with latex was Y =1.336X + 0.3906.The determination coefficient was 0.9995.The regression equation of homocysteine measured by high performance liquid chromatography was Y =0.021X-0.0085.The determination coefficient was 0.9999.Conclusions The nephelometry assay with latex is accurate,sensitive,rapid,specific and suitable for the routine clinical practice and the research practice.