CAJ | 학술논문

目的研究多粘菌素B对大鼠移植肺中Bcl-2表达的影响,探讨缺血预适应对大鼠移植肺保护的机制.方法在大鼠左肺移植模型基础上分别采用缺血预适应及多粘菌素B灌注肺动脉,观察对移植肺Bcl-2表达的影响.结果缺血预适应组与对照组、多粘菌素B组左肺静脉血氧分压、细胞凋亡指数、Bcl-2表达比较差异有统计学意义(P＜0.05),对照组与多粘菌素B组比较差异无统计学意义(P＞0.05).结论肺组织中缺血预适应保护机制可能为蛋白激酶C活化；多粘菌素B能使蛋白激酶C失活,导致Bcl-2表达下调,引起细胞凋亡.
목적 연구다점균소B대대서이식폐중Bcl-2표체적영향,탐토결혈예괄응대대서이식폐보호적궤제.방법 재대서좌폐이식모형기출상분별채용결혈예괄응급다점균소B관주폐동맥,관찰대이식폐Bcl-2표체적영향.결과 결혈예괄응조여대조조、다점균소B조좌폐정맥혈양분압、세포조망지수、Bcl-2표체비교차이유통계학의의(P＜0.05),대조조여다점균소B조비교차이무통계학의의(P＞0.05).결론 폐조직중결혈예괄응보호궤제가능위단백격매C활화；다점균소B능사단백격매C실활,도치Bcl-2표체하조,인기세포조망.
Objective To study the effect of ploymyxin B on Bcl-2 expression,and the protective mechanism of ischemic preconditioning on rat lung transplantation.Methods Based on the model of lung transplantation in rat,ischemic preconditioning and ploymyxin B was used,respectively,and the effects on expression of Bcl-2 in transplanted lung were observed.Results Compared with ploymyxin B group and control group,the blood oxygen of left pulmonary veins,apoptosis index and the expression of Bcl-2 of ischemic preconditioning group were significantly different (P ＜ 0.05),there was no significant difference between ploymyxin B group and control group (P ＞ 0.05).Conclusions Lung protective mechanism of ischemic preconditioning may be protein kinase C activation;Polymyxin B can make PKC deactivation,thereby down-regulation the expression of Bcl-2,and then arouse the apoptosis of the cells.