国际病毒学杂志
國際病毒學雜誌
국제병독학잡지
INTERNATIONAL JOURNAL OF VIROLOGY
2013年
6期
265-269
,共5页
罗慧琴%王志刚%李玲%刘付芹
囉慧琴%王誌剛%李玲%劉付芹
라혜금%왕지강%리령%류부근
乙肝标志物%HBV-DNA%荧光定量PCR
乙肝標誌物%HBV-DNA%熒光定量PCR
을간표지물%HBV-DNA%형광정량PCR
Hepatitis B markers%HBV-DNA%Fluorescence quantitative PCR
目的 探讨乙肝血清标志物与病毒DNA水平之间的相关性.方法 采用实时荧光定量PCR对874例HBV感染者血清中HBV-DNA含量进行检测,同时运用ELISA法检测HBV血清标志物,并统计分析患者乙型肝炎血清标志物、病毒DNA之间的相关性及分布特点.结果 在受检的874例标本中,男性533例,阳性率58.16％ (310/533);女性341例,阳性率50.44％(172/341),男性和女性阳性率比较差异有统计学意义(X2=5.01,P＜0.05),男性和女性HBV-DNA水平差异无统计学意义(t =0.117,P=0.907).乙肝总阳性率和HBV-DNA水平均随年龄的增长呈下降趋势,不同年龄组间比较差异有统计学意义.同一年龄段的大三阳与小三阳、两头阳和三抗阳之间比较差异有统计学意义;其中男性和女性HBV-DNA水平随年龄增长均呈下降趋势,差异有统计学意义.≤20岁以下人群HBV-DNA阳性率最高达82.86％.结论 HBV-DNA阳性率和HBV-DNA水平都随年龄增长呈下降趋势,其中≤20岁年龄段HBV-DNA阳性率最高达82.86％;男性HBV-DNA的阳性率高于女性.
目的 探討乙肝血清標誌物與病毒DNA水平之間的相關性.方法 採用實時熒光定量PCR對874例HBV感染者血清中HBV-DNA含量進行檢測,同時運用ELISA法檢測HBV血清標誌物,併統計分析患者乙型肝炎血清標誌物、病毒DNA之間的相關性及分佈特點.結果 在受檢的874例標本中,男性533例,暘性率58.16％ (310/533);女性341例,暘性率50.44％(172/341),男性和女性暘性率比較差異有統計學意義(X2=5.01,P＜0.05),男性和女性HBV-DNA水平差異無統計學意義(t =0.117,P=0.907).乙肝總暘性率和HBV-DNA水平均隨年齡的增長呈下降趨勢,不同年齡組間比較差異有統計學意義.同一年齡段的大三暘與小三暘、兩頭暘和三抗暘之間比較差異有統計學意義;其中男性和女性HBV-DNA水平隨年齡增長均呈下降趨勢,差異有統計學意義.≤20歲以下人群HBV-DNA暘性率最高達82.86％.結論 HBV-DNA暘性率和HBV-DNA水平都隨年齡增長呈下降趨勢,其中≤20歲年齡段HBV-DNA暘性率最高達82.86％;男性HBV-DNA的暘性率高于女性.
목적 탐토을간혈청표지물여병독DNA수평지간적상관성.방법 채용실시형광정량PCR대874례HBV감염자혈청중HBV-DNA함량진행검측,동시운용ELISA법검측HBV혈청표지물,병통계분석환자을형간염혈청표지물、병독DNA지간적상관성급분포특점.결과 재수검적874례표본중,남성533례,양성솔58.16％ (310/533);녀성341례,양성솔50.44％(172/341),남성화녀성양성솔비교차이유통계학의의(X2=5.01,P＜0.05),남성화녀성HBV-DNA수평차이무통계학의의(t =0.117,P=0.907).을간총양성솔화HBV-DNA수평균수년령적증장정하강추세,불동년령조간비교차이유통계학의의.동일년령단적대삼양여소삼양、량두양화삼항양지간비교차이유통계학의의;기중남성화녀성HBV-DNA수평수년령증장균정하강추세,차이유통계학의의.≤20세이하인군HBV-DNA양성솔최고체82.86％.결론 HBV-DNA양성솔화HBV-DNA수평도수년령증장정하강추세,기중≤20세년령단HBV-DNA양성솔최고체82.86％;남성HBV-DNA적양성솔고우녀성.
Objective To investigate the correlation between HBV serum markers and HBV DNA levels.Methods HBV-DNA and serum markers in 874 cases of HBV infected persons were detected by real-time fluorescence quantitative PCR and ELISA,respectively.SPSS 16.0 was used to analyze the correlation and distribution characteristics.Results In 874 cases,533 positive cases were male,the positive rate was 58.16％ (310/533).341 positive cases were female,the positive rate was 50.44％ (172/341).There was statistical difference (x2 =5.01,P ＜0.05) comparing male with female.But there was no statistical difference (t =0.117,P =0.907) in HBV-DNA level between male and female.The total positive rate and HBV-DNA level showed descending tendency with age,and there were statistical differences among different age groups.When comparing big 3 this world,small 3 this world,two head positive and three antibody positive among the same age group,the statistical differences were found.The HBV-DNA level in both male and female showed descending tendency with age increasing,the statistical difference was found.20 years old age group showed the highest positive rate (82.86％).Conclusions The positive rate of HBVDNA and HBV-DNA level deceased with the age increasing.≤ 20 years old age group showed the highest HBV-DNA positive rate(82.86％).HBV-DNA positive rate of the male was higher than the female.
