国际儿科学杂志
國際兒科學雜誌
국제인과학잡지
INTERNATIONAL JOURNAL OF PEDIATRICS
2013年
2期
201-203,207
,共4页
高氧%胎鼠远端肺上皮细胞%液体转运%上皮钠通道
高氧%胎鼠遠耑肺上皮細胞%液體轉運%上皮鈉通道
고양%태서원단폐상피세포%액체전운%상피납통도
Hyperoxia%Fetal distal lung epithelia%Fluid transport%Epithelial sodium channel
目的 探讨高氧对胎鼠远端肺上皮(FDLE)液体转运功能及上皮钠通道(ENaC)表达的影响.方法 分离提取大鼠FDLE细胞,随机分成高氧组和空气组,分别在氧浓度为85％和21％的细胞培养箱中原代培养.检测高氧暴露24h和48 h经FDLE细胞单层液体转运量的变化.应用Western blot方法检测高氧暴露后α-ENaC蛋白表达的变化.结果 高氧48h使大鼠FDLE液体转运增加(1.78±0.19 vs.1.06±0.11,P＜0.001),并且该促进作用可被阿米洛利抑制.高氧24h FDLE中α-ENaC蛋白表达与空气组比较差异无统计学意义(0.44 +0.04 vs.0.40±0.04,P=0.22),而高氧48h α-ENaC蛋白表达显著低于空气组(0.35±0.03 vs.0.47±0.06,P=0.03).结论 高氧增强了FDLE液体转运功能,且以阿米洛利敏感性液体转运增加为主.该促进作用并非通过增加α-ENaC蛋白表达来实现.
目的 探討高氧對胎鼠遠耑肺上皮(FDLE)液體轉運功能及上皮鈉通道(ENaC)錶達的影響.方法 分離提取大鼠FDLE細胞,隨機分成高氧組和空氣組,分彆在氧濃度為85％和21％的細胞培養箱中原代培養.檢測高氧暴露24h和48 h經FDLE細胞單層液體轉運量的變化.應用Western blot方法檢測高氧暴露後α-ENaC蛋白錶達的變化.結果 高氧48h使大鼠FDLE液體轉運增加(1.78±0.19 vs.1.06±0.11,P＜0.001),併且該促進作用可被阿米洛利抑製.高氧24h FDLE中α-ENaC蛋白錶達與空氣組比較差異無統計學意義(0.44 +0.04 vs.0.40±0.04,P=0.22),而高氧48h α-ENaC蛋白錶達顯著低于空氣組(0.35±0.03 vs.0.47±0.06,P=0.03).結論 高氧增彊瞭FDLE液體轉運功能,且以阿米洛利敏感性液體轉運增加為主.該促進作用併非通過增加α-ENaC蛋白錶達來實現.
목적 탐토고양대태서원단폐상피(FDLE)액체전운공능급상피납통도(ENaC)표체적영향.방법 분리제취대서FDLE세포,수궤분성고양조화공기조,분별재양농도위85％화21％적세포배양상중원대배양.검측고양폭로24h화48 h경FDLE세포단층액체전운량적변화.응용Western blot방법검측고양폭로후α-ENaC단백표체적변화.결과 고양48h사대서FDLE액체전운증가(1.78±0.19 vs.1.06±0.11,P＜0.001),병차해촉진작용가피아미락리억제.고양24h FDLE중α-ENaC단백표체여공기조비교차이무통계학의의(0.44 +0.04 vs.0.40±0.04,P=0.22),이고양48h α-ENaC단백표체현저저우공기조(0.35±0.03 vs.0.47±0.06,P=0.03).결론 고양증강료FDLE액체전운공능,차이아미락리민감성액체전운증가위주.해촉진작용병비통과증가α-ENaC단백표체래실현.
Objective To investigate the effect of hyperoxia on fluid transport by fetal distal lung epithelia(FDLE) and the expression of epithelial sodium channel(ENaC) in these cells.Methods FDLE were isolated and randomized into hyperoxia group and normoxia group,which were primarily cultured under hyperoxic or normoxic conditions,respectively.Fluid transport was measured using monolayers of FDLE cultured on Transwell permeable inserts.Western blot was applied to examine the α-ENaC expression.Results Fluid transport across monolayer of FDLE was increased in cells exposed to hyperoxia compaired with cells cultured in normoxic conditions (1.78 ± 0.19 vs 1.06 ± 0.11,P ＜ 0.001).Amiloride significantly decreased the fluid transport in both of the hyperoxia and normoxia groups,but in the presence of amiloride there were no difference between the two groups.The expression of α-ENaC was inhibited by hyperoxia to some extent(24h:0.44 ±0.04 vs 0.40 ±0.04,P=0.22; 48h:0.35 ±0.03 vs 0.47 ±0.06,P =0.03).Conclusion Hyperoxia enhanced total and amiloride-sensitive fluid transport by FDLE.However,the expression of α-ENaC decreased in these cells.
