CAJ | 학술논문

目的比较小鼠高氧肺损伤肺组织与正常肺组织的差异表达基因,从分子水平揭示高氧肺损伤的发病机制.方法利用PubMed数据库获取小鼠高氧肺损伤肺组织与正常肺组织的差异表达基因,并进行生物学通路、基因本体和功能注释聚类等生物信息分析.结果两组间差异表达基因共467条,以2倍标准筛选出189条差异表达基因,再用功能注释工具将超过2倍差异表达的基因对KEGG数据库进行映射,共有54条基因注释到KEGG数据库的小鼠生物学通路中.通过代谢通路的富集度分析,有5条通路显示具有相关性,提示这些途径可能和高氧肺损伤密切相关.5条通路中与高氧肺损伤相关基因20条(上调基因14条,下调基因6条),它们在造血细胞的增殖、分化、更新,轴突导向,细胞之间的黏着连接,T细胞受体信号转导通路,黏着斑形成方面起着重要作用.结论对挖掘出的20条与高氧肺损伤相关的基因进行深入研究,将为揭示高氧肺损伤的分子机制和靶向治疗提供有效方法.
목적 비교소서고양폐손상폐조직여정상폐조직적차이표체기인,종분자수평게시고양폐손상적발병궤제.방법 이용PubMed수거고획취소서고양폐손상폐조직여정상폐조직적차이표체기인,병진행생물학통로、기인본체화공능주석취류등생물신식분석.결과 량조간차이표체기인공467조,이2배표준사선출189조차이표체기인,재용공능주석공구장초과2배차이표체적기인대KEGG수거고진행영사,공유54조기인주석도KEGG수거고적소서생물학통로중.통과대사통로적부집도분석,유5조통로현시구유상관성,제시저사도경가능화고양폐손상밀절상관.5조통로중여고양폐손상상관기인20조(상조기인14조,하조기인6조),타문재조혈세포적증식、분화、경신,축돌도향,세포지간적점착련접,T세포수체신호전도통로,점착반형성방면기착중요작용.결론 대알굴출적20조여고양폐손상상관적기인진행심입연구,장위게시고양폐손상적분자궤제화파향치료제공유효방법.
Objective To explore the pathogenesis mechanism of hyperoxic lung injury and the effective means for its clinical treatment,the difference of the gene expressions between lung tissues of hyperoxic lung injury and normal lung was compared.Methods The differentially expressed genes between lung tissues of hyperoxic lung injury and normal lung were obtained from PubMed.The dysregulated genes in lung tissues of hyperoxic lung injury were analyzed by a series of bioinformatics methods,including pathways,gene ontology and functional annotation clustering analysis.Results 467 lines of differentially expressed genes were found and genes more than 2-fold regulated were 189.We sought the mapping of genes in the KEGG databases through functional annotation tools,and we discovered there were 5 lines of pathways with difference having outstangding statistical significance through metabolic pathways enrichment degree analysis.It reflected the pathways were closely related to hyperoxic lung injury (the 2-fold upregulated genes were 14,the 2-fold down-regulated genes were 6).GO analysis revealed that these genes were involved in hematopietic cell lineage,axon guidance,adherens junction,T cell receptor signaling pathway and focal adhesion.Conclusions Therefore,it is believed that the above-mentioned 20 lines of gnes are the major ones for the hyperoxic lung injury and the research on them will provide effective means for revealing the molecular mechanism of hyperoxic lung injury and identifying the targeted therapy.