CAJ | 학술논문

目的通过观察Graves病患者外周血中CD4+CD25+CD127-调节性T细胞(Treg)数量的变化,探讨Graves病发生的免疫学机制.方法采用流式细胞术检测90例Graves病患者和50名正常人外周血中CD4+CD25+CD127-Treg的比例,同时测定甲状腺功能指标及甲状腺自身抗体水平.采用t检验进行组间比较,采用线性相关分析法分析CD4+CD25+CD127-Treg与甲状腺功能指标的关系.结果 90例Graves病患者与50名正常人外周血中CD4+CD25+CD127-Treg的比例分别为1.39％±1.09％和4.59％±1.14％,两者间差异有统计学意义(t=16.4,P＜0.01).CD4+CD25+CD127-Treg数量与促甲状腺激素受体抗体水平呈负相关(r=-0.24,P＜0.05),与总三碘甲状腺原氨酸、总甲状腺素、游离三碘甲状腺原氨酸、游离甲状腺素、甲状腺球蛋白抗体、甲状腺微粒体抗体水平呈负相关(r=-0.62、-0.65、-0.56、-0.71、-0.50、-0.15,P均＜0.01).结论 Graves病患者CD4+CD25+CD127-Treg 数量减少,且CD4+CD25+CD127-Treg数量与甲状腺功能指标及甲状腺自身抗体水平关系密切,提示CD4+CD25+CD127-Treg数量减少可能与Graves病的发病有关.CD4+CD25+CD127-可能是人CD4+T 细胞中Treg的特异性标志物.
목적 통과관찰Graves병환자외주혈중CD4+CD25+CD127-조절성T세포(Treg)수량적변화,탐토Graves병발생적면역학궤제.방법 채용류식세포술검측90례Graves병환자화50명정상인외주혈중CD4+CD25+CD127-Treg적비례,동시측정갑상선공능지표급갑상선자신항체수평.채용t검험진행조간비교,채용선성상관분석법분석CD4+CD25+CD127-Treg여갑상선공능지표적관계.결과 90례Graves병환자여50명정상인외주혈중CD4+CD25+CD127-Treg적비례분별위1.39％±1.09％화4.59％±1.14％,량자간차이유통계학의의(t=16.4,P＜0.01).CD4+CD25+CD127-Treg수량여촉갑상선격소수체항체수평정부상관(r=-0.24,P＜0.05),여총삼전갑상선원안산、총갑상선소、유리삼전갑상선원안산、유리갑상선소、갑상선구단백항체、갑상선미립체항체수평정부상관(r=-0.62、-0.65、-0.56、-0.71、-0.50、-0.15,P균＜0.01).결론 Graves병환자CD4+CD25+CD127-Treg 수량감소,차CD4+CD25+CD127-Treg수량여갑상선공능지표급갑상선자신항체수평관계밀절,제시CD4+CD25+CD127-Treg수량감소가능여Graves병적발병유관.CD4+CD25+CD127-가능시인CD4+T 세포중Treg적특이성표지물.
Objective To investigate the mechanism of Graves disease by observing the changes of CD4+CD25+CD127-regulatory T cells (Treg) population in the patients.Methods Flow cytometry was used to detect the proportion of CD4+CD25+CD127-Treg of CD4+ T cells in 90 Graves disease patients (Graves disease group) and 50 healthy adults(control group).Thyroid function and autoantibody levels were determined simultaneously.The t test was adopted for comparison between groups.The relationship between CD4+CD25+CD127-Treg and thyroid function was analyzed by linear correlation analysis.Results The percentages of CD4+CD25+CD127-Treg in Graves disease group and control group were 1.39％±1.09％ and 4.59％±1.14％ separately.There was significant difference between the two groups (t=16.4,P＜0.01).There were negative correlation between CD4+CD25+CD127-Treg percentages and total triiodothyronine,total thyroxine,free triiodothyronine,free thyroxine and thyrotropin receptor antibody,thyroglobulin antibody,thyroid microsomal antibody (r=-0.62,-0.65,-0.56,-0.71,-0.50,-0.15,all P＜0.01).Conclusions The reduction of CD4+CD25+CD127-Treg percentages in Graves disease group and close relations of CD4+CD25+CD127-Treg with thyroid function and thyroid autoantibody levels suggest that CD4+CD25+CD127-Treg decrease in the number may be associated with the onset of Graves disease.CD4+CD25+CD127-may be the specific marker of Treg.