CAJ | 학술논문

目的探讨不同剂量γ射线照射对不同品系小鼠造血功能的影响.方法用137Csγ射线分别对IRM-2、ICR、615品系小鼠进行一次性全身4.0 Gyγ射线照射,在照后不同时间检测小鼠外周血白细胞、骨髓有核细胞(BMNC)的变化.对IRM-2小鼠、C57BL/6小鼠进行6.0 Gyγ射线照射,照后第45日,检测小鼠外周血象的变化.结果 IRM-2、ICR、615品系小鼠经4.0 Gy照射后第2日,白细胞和BMNC数量降至最低值.第9日,IRM-2小鼠的BMNC计数与ICR、615小鼠相比,差异有统计学意义(t=3.725,P＜0.01；t=8.487,P＜0.001).第12日,IRM-2小鼠白细胞计数与ICR、615小鼠相比,差异有统计学意义(t=4.811和4.302,P均＜0.001).第21日,IRM-2、ICR、615小鼠白细胞计数分别恢复到正常值的52.0％、60.7％、50.8％；BMNC计数分别恢复到正常值的90.8％、82.1％、75.4％.IRM-2、C57BL/6小鼠经6.0 Gy γ射线照射后第45日,IRM-2小鼠外周血白细胞、红细胞、血红蛋白(Hgb)、红细胞压积(Hct)计数均明显高于C57BL/6小鼠(t=5.629、7.788、4.929和6.064,P均＜0.001)；IRM-2小鼠白细胞、红细胞、Hgb、Hct数量分别恢复至正常值的75.00％、98.95％、98.78％、97.55％；C57BL/6小鼠白细胞、红细胞、Hgb、Hct数量分别恢复至正常值的40.60％、93.88％、93.31％、93.84％.结论 IRM-2、ICR、615、C57BL/6小鼠受照后造血功能的恢复趋势相同,但IRM-2小鼠在辐射损伤后其造血功能的恢复较快于ICR、615、C57BL/6小鼠.
목적 탐토불동제량γ사선조사대불동품계소서조혈공능적영향.방법 용137Csγ사선분별대IRM-2、ICR、615품계소서진행일차성전신4.0 Gyγ사선조사,재조후불동시간검측소서외주혈백세포、골수유핵세포(BMNC)적변화.대IRM-2소서、C57BL/6소서진행6.0 Gyγ사선조사,조후제45일,검측소서외주혈상적변화.결과 IRM-2、ICR、615품계소서경4.0 Gy조사후제2일,백세포화BMNC수량강지최저치.제9일,IRM-2소서적BMNC계수여ICR、615소서상비,차이유통계학의의(t=3.725,P＜0.01；t=8.487,P＜0.001).제12일,IRM-2소서백세포계수여ICR、615소서상비,차이유통계학의의(t=4.811화4.302,P균＜0.001).제21일,IRM-2、ICR、615소서백세포계수분별회복도정상치적52.0％、60.7％、50.8％；BMNC계수분별회복도정상치적90.8％、82.1％、75.4％.IRM-2、C57BL/6소서경6.0 Gy γ사선조사후제45일,IRM-2소서외주혈백세포、홍세포、혈홍단백(Hgb)、홍세포압적(Hct)계수균명현고우C57BL/6소서(t=5.629、7.788、4.929화6.064,P균＜0.001)；IRM-2소서백세포、홍세포、Hgb、Hct수량분별회복지정상치적75.00％、98.95％、98.78％、97.55％；C57BL/6소서백세포、홍세포、Hgb、Hct수량분별회복지정상치적40.60％、93.88％、93.31％、93.84％.결론 IRM-2、ICR、615、C57BL/6소서수조후조혈공능적회복추세상동,단IRM-2소서재복사손상후기조혈공능적회복교쾌우ICR、615、C57BL/6소서.
Objective To discuss the effects of different dose of radiation on the mouse's hematopoietic system.Methods Mice of 615 strain,ICR strain and IRM-2 strain were given a one-time 4.0 Gy total body irradiation,and then the changes of peripheral white blood cells and bone marrow nuclear cells (BMNC)among those mice were observed at different time points.Mice of IRM-2 and C57BL/6 were given a one-time 6.0 Gy total body irradiation and the changes of peripheral hematological between the two strains were observed forty-five days after irradiation.Results Two days after irradiation,white blood cell and BMNC counts decreased to the lowest level in all the mice of 615 strain,ICR strain and IRM-2 strain.During the following days,white blood cell and BMNC counts raised again.Nine days after irradiation,BMNC count in IRM-2 mice was significantly higher than that in 615 and ICR mice(t=3.725,P＜0.01; t=8.487,P＜0.001).Twelve days after irradiation,white blood cell count in IRM-2 mice was significantly higher than that in the 615 and ICR mice (t=4.811 and 4.302,both P ＜0.001).Twenty-one days after irradiation,compared to the control group,white blood cell count was recovered to 52.0％,60.7％ and 50.8％ of the normal range in IRM-2,ICR and 615 mice separately and BMNC count was recovered to 90.8％,82.1％,75.4％ of the normal range in IRM-2,ICR and 615 mice separately.Forty-five days after 6.0 Gy irradiation,white blood cell,BMNC,hemoglobin and hematocrit counts in IRM-2 mice were significantly higher than those indexes in C57BL/6strain(t=5.629,7.788,4.9289,6.064,all P＜0.001).Compared to the control group,white blood cells,red blood cells,hemoglobin and hematocrit were recovered to 75.0％,98.9％,98.7％,and 97.5％ of the normal range separately in IRM-2 mice.And white blood cells,red blood cells,hemoglobin and hematocrit were recovered to 40.6％,93.8％,93.3％,and 93.8％ of the normal range separately in C57BL/6 mice.Conclusion There are similar recovery trends of hematopoiesis in all strains of mice after irradiation,but IRM-2 mice recovery more quickly than the other strains of mice.