CAJ | 학술논문

目的应用单细胞凝胶电泳(SCGE)和微核实验探讨角质细胞生长因子(KGF)对受照小鼠的遗传保护作用.方法将40只C57BL/6J小鼠随机分为空白对照组、照射对照组、KGF低(1 mg/kg)、中(2 mg/kg)、高(4 mg/kg)剂量组5组.KGF的3个给药组于照射前给药,连续3d分别给予腹腔注射1、2、4 mg/kg的KGF,1次/d.照射对照组腹腔注射磷酸盐缓冲液.给药后,空白对照组给予假照射,其他4组给予7.2 Gy 137Csγ射线全身一次性照射.应用SCGE和微核实验检测5组小鼠的DNA损伤情况.结果与照射对照组相比,KGF低剂量组小鼠的DNA微核率显著降低,中、高剂量组的彗星尾矩、Olive尾矩和微核率均显著降低.结论 KGF对受照小鼠有显著的遗传保护作用.
목적 응용단세포응효전영(SCGE)화미핵실험탐토각질세포생장인자(KGF)대수조소서적유전보호작용.방법 장40지C57BL/6J소서수궤분위공백대조조、조사대조조、KGF저(1 mg/kg)、중(2 mg/kg)、고(4 mg/kg)제량조5조.KGF적3개급약조우조사전급약,련속3d분별급여복강주사1、2、4 mg/kg적KGF,1차/d.조사대조조복강주사린산염완충액.급약후,공백대조조급여가조사,기타4조급여7.2 Gy 137Csγ사선전신일차성조사.응용SCGE화미핵실험검측5조소서적DNA손상정황.결과 여조사대조조상비,KGF저제량조소서적DNA미핵솔현저강저,중、고제량조적혜성미구、Olive미구화미핵솔균현저강저.결론 KGF대수조소서유현저적유전보호작용.
Objective To study the protective effect of keratinocyte growth factor (KGF) in genetics damage induced by radiation using the methods of single cell gel electrophoresis (SCGE) and micronuclei assay.Methods The C57BL/6J mice were randomly divided into five groups:control group,radiation group and low,middle,high dose groups of KGF(1,2,4 mg/kg).Drugs were administrated by intraperitoneal injection.Then control group was given sham-irradiated,and other groups were given a whole body irradiation at the dose of 7.2 Gy with γ-rays.DNA damage of different groups were detected by SCGE and micronuclei assay.Results Compared with radiation group,pretreatment with KGF can significantly decrease the tail moment and micronucleus formation.Conclusion Pretreatment with KGF can effectively protect the radiation-induced genetics damage in mouse model.