国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2013年
2期
84-87
,共4页
林琳%方平%封国红%糜叶俊
林琳%方平%封國紅%糜葉俊
림림%방평%봉국홍%미협준
非小细胞肺癌%表皮生长因子受体%突变
非小細胞肺癌%錶皮生長因子受體%突變
비소세포폐암%표피생장인자수체%돌변
Non-small cell lung cancer%Epidermal growth factor receptor%Mutation
目的 探索铜陵地区非小细胞肺癌表皮生长因子受体(EGFR)基因突变与其临床病理特征的关系.方法 用蝎形探针扩增阻滞突变系统对非小细胞肺癌患者石蜡包埋组织、冷冻组织进行EGFR基因18～21外显子突变的检测,分析EGFR基因的突变的分布特征及其与临床病理特征的相关性.结果 42份非小细胞肺癌标本中24份检测出EGFR基因突变,总检出率57.14％.突变类型主要包括L858R点突变(41.67％)、19-del E746-A450缺失突变(41.67％)、L858R+ 19-del(12.50％)、G719X点突变(4.17％).未检测出第20外显子T790M点突变.女性患者突变率(75.00％)较男性患者突变率(40.91％)高,二者差异有统计学意义(P=0.026)；吸烟＜400年支突变率为70.37％,较吸烟≥400年支突变率(33.33％)高,差异有统计学意义(P=0.02)；肿瘤病理学类型为腺癌突变率为60.61％(20/33),而鳞癌突变率为40.00％ (2/5),二者差异无统计学意义(P=0.346).年龄＜60岁患者突变率为64.00％,较≥60岁患者突变率(47.06％)高,差异无统计学意义(P=0.276).不同TNM分期之间突变率差异无统计学意义(P=0.9).结论 EGFR基因突变多见于女性、不吸烟或少吸烟非小细胞肺癌患者.
目的 探索銅陵地區非小細胞肺癌錶皮生長因子受體(EGFR)基因突變與其臨床病理特徵的關繫.方法 用蝎形探針擴增阻滯突變繫統對非小細胞肺癌患者石蠟包埋組織、冷凍組織進行EGFR基因18～21外顯子突變的檢測,分析EGFR基因的突變的分佈特徵及其與臨床病理特徵的相關性.結果 42份非小細胞肺癌標本中24份檢測齣EGFR基因突變,總檢齣率57.14％.突變類型主要包括L858R點突變(41.67％)、19-del E746-A450缺失突變(41.67％)、L858R+ 19-del(12.50％)、G719X點突變(4.17％).未檢測齣第20外顯子T790M點突變.女性患者突變率(75.00％)較男性患者突變率(40.91％)高,二者差異有統計學意義(P=0.026)；吸煙＜400年支突變率為70.37％,較吸煙≥400年支突變率(33.33％)高,差異有統計學意義(P=0.02)；腫瘤病理學類型為腺癌突變率為60.61％(20/33),而鱗癌突變率為40.00％ (2/5),二者差異無統計學意義(P=0.346).年齡＜60歲患者突變率為64.00％,較≥60歲患者突變率(47.06％)高,差異無統計學意義(P=0.276).不同TNM分期之間突變率差異無統計學意義(P=0.9).結論 EGFR基因突變多見于女性、不吸煙或少吸煙非小細胞肺癌患者.
목적 탐색동릉지구비소세포폐암표피생장인자수체(EGFR)기인돌변여기림상병리특정적관계.방법 용갈형탐침확증조체돌변계통대비소세포폐암환자석사포매조직、냉동조직진행EGFR기인18～21외현자돌변적검측,분석EGFR기인적돌변적분포특정급기여림상병리특정적상관성.결과 42빈비소세포폐암표본중24빈검측출EGFR기인돌변,총검출솔57.14％.돌변류형주요포괄L858R점돌변(41.67％)、19-del E746-A450결실돌변(41.67％)、L858R+ 19-del(12.50％)、G719X점돌변(4.17％).미검측출제20외현자T790M점돌변.녀성환자돌변솔(75.00％)교남성환자돌변솔(40.91％)고,이자차이유통계학의의(P=0.026)；흡연＜400년지돌변솔위70.37％,교흡연≥400년지돌변솔(33.33％)고,차이유통계학의의(P=0.02)；종류병이학류형위선암돌변솔위60.61％(20/33),이린암돌변솔위40.00％ (2/5),이자차이무통계학의의(P=0.346).년령＜60세환자돌변솔위64.00％,교≥60세환자돌변솔(47.06％)고,차이무통계학의의(P=0.276).불동TNM분기지간돌변솔차이무통계학의의(P=0.9).결론 EGFR기인돌변다견우녀성、불흡연혹소흡연비소세포폐암환자.
Objective To investigate the epidermal growth factor receptor (EGFR) gene mutation and related clinicopathological features in patients with non small cell lung cancer (NSCLC) in Tongling region.Methods Amplification refractory mutation system was applied to detect EGFR mutations involving exons 18-21 in paraffin imbedding and refrigeration tissue specimens.The relationship between EGFR mutations and clinicopathological features was analyzed.Results 24 specimens were detected EGFR gene mutation in 42 NSCLC specimens.The overall EGFR mutation detection rate was 57.14％.The EGFR mutation types included L858R mutation (41.67％),19-del E746-A450 mutation (41.67％),L858R combined with 19-del mutation (12.50％),and G719X mutation (4.17％).T790M mutation in exon 21 was not found in this group of patients.EGFR mutation rate was higher in female patients (75.00％) than that in male patients (40.91％,P =0.026).EGFR mutation rate was higher in smoking ＜400 packets · year group (70.37％) than that in smoking ≥400 packets · year group (33.33％,P =0.02).EGFR gene mutation rate was 60.61％ in lung adenocarcinoma,and that was 40.00％ in squamous cell carcinoma (P =0.346).There was no significant difference on EGFR mutation rate between age ＜60 years old patients and age ≥60 years old patients (64.00％ vs 47.06％,P =0.276).There was no significant correlation of EGFR mutation with TNM staging (P =0.9).Conclusions There is a higher EGFR mutation rate in female,non-smoker or less smoker.
