CAJ | 학술논문

目的观察激素抵抗性哮喘(SRA)患者外周血CD4+ CD25+ Foxp3+调节性T细胞(Treg)及白介素10(IL-10)、转化生长因子β1(TGF-β1)的变化,分析其在SRA发病机制中的作用.方法采用流式细胞术检测40例SRA患者(激素抵抗组)外周血单个核细胞CD4+ CD25+ Foxp3+ Treg数目,并计算CD4+ CD25+ Foxp3+ Treg占CD4+T淋巴细胞的百分比；酶联免疫吸附试验(ELISA)法检测其血清IL-10、TGF-β1水平,并与激素敏感性患者(激素敏感组,46例)及正常体检者(正常组,30例)进行对比.结果激素抵抗组患者外周血CD4+ CD25+ Foxp3+ Treg占CD4+T淋巴细胞的百分比、CD4+ CD25+Foxp3+ Treg绝对值及血清IL-10、TGF-β1水平均明显低于激素敏感组与正常组(P＜0.01,P＜0.05)；激素敏感组患者外周血CD4+ CD25+ Foxp3+ Treg占CD4+T淋巴细胞的百分比、CD4+ CD25+ Foxp3+Treg绝对值及血清TGF-β1水平明显低于正常组(P＜0.01,P＜0.05),血清IL-10无明显差异(P＞0.05)；CD4+ CD25+ Foxp3+ Treg/CD4+T及CD4+ CD25+ Foxp3+ Treg绝对数均与血清IL-10、TGF-β1水平呈明显正相关(P＜0.01).结论 SRA患者外周血CD4+ CD25+ Foxp3+ Treg数目减少及IL-10、TGF-β1含量减低可能与SRA的发生、发展有关.
목적 관찰격소저항성효천(SRA)환자외주혈CD4+ CD25+ Foxp3+조절성T세포(Treg)급백개소10(IL-10)、전화생장인자β1(TGF-β1)적변화,분석기재SRA발병궤제중적작용.방법 채용류식세포술검측40례SRA환자(격소저항조)외주혈단개핵세포CD4+ CD25+ Foxp3+ Treg수목,병계산CD4+ CD25+ Foxp3+ Treg점CD4+T림파세포적백분비；매련면역흡부시험(ELISA)법검측기혈청IL-10、TGF-β1수평,병여격소민감성환자(격소민감조,46례)급정상체검자(정상조,30례)진행대비.결과 격소저항조환자외주혈CD4+ CD25+ Foxp3+ Treg점CD4+T림파세포적백분비、CD4+ CD25+Foxp3+ Treg절대치급혈청IL-10、TGF-β1수평균명현저우격소민감조여정상조(P＜0.01,P＜0.05)；격소민감조환자외주혈CD4+ CD25+ Foxp3+ Treg점CD4+T림파세포적백분비、CD4+ CD25+ Foxp3+Treg절대치급혈청TGF-β1수평명현저우정상조(P＜0.01,P＜0.05),혈청IL-10무명현차이(P＞0.05)；CD4+ CD25+ Foxp3+ Treg/CD4+T급CD4+ CD25+ Foxp3+ Treg절대수균여혈청IL-10、TGF-β1수평정명현정상관(P＜0.01).결론 SRA환자외주혈CD4+ CD25+ Foxp3+ Treg수목감소급IL-10、TGF-β1함량감저가능여SRA적발생、발전유관.
Objective To investigate the changes of CD4+ CD25+ Foxp3+ regulatory T cell and IL-10,TGF-β1 in steroid-resistant asthma patients of peripheral blood,to study their significance.Methods The CD4+ CD25+ Foxp3+ regulatory T cell in 40 cases steroid-resistant asthma patients were detected by flow cytometry.The levels of serum IL-10 and TGF-β1 were detected by ELISA.The control groups were 30 individuals having general physical examination and 46 patients with steroid-sensitive patients.Results The proportion and number of CD4+ CD25+ Foxp3+ regulatory T cell and the levels of serum IL-10,TGF-β1 in steroid-resistant asthma group of Peripheral Blood were decreased significantly than steroid-sensitive group and healthy control group (P ＜0.01,P ＜0.05).The proportion and number of CD4+CD25+ Foxp3+ regulatory T cell and the levelsof serum TGF--β1 in steroid-sensitive group were decreased significantly than healthy control group,but the levels of serum IL-10 were no statistical difference between them.The proportion and number of CD4+ CD25+ Foxp3+ regulatory T cell were positively correlated with the serum IL-10,TGF-β1 (P ＜0.01).Conclusions Lower proportion and number of CD4+ CD25+ Foxp3+ regulatory T cell and lower IL-10,TGF-β1 were observed in steroid-resistant asthma patients,which might play an important role in occurrence and development of steroid-resistant asthma.