国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2013年
3期
180-184
,共5页
张金梅%孙丽华%谷伟%谭焰
張金梅%孫麗華%穀偉%譚燄
장금매%손려화%곡위%담염
慢性阻塞性肺疾病%噻托溴铵%肺表面活性蛋白-D
慢性阻塞性肺疾病%噻託溴銨%肺錶麵活性蛋白-D
만성조새성폐질병%새탁추안%폐표면활성단백-D
Chronic obstructive pulmonary disease%Tiotropium bromide%Pulmonary surfactant protein D
目的 通过研究噻托溴铵对慢性阻塞性肺疾病(COPD)大鼠血清及肺组织中肺表面活性蛋白-D(pulmonary surfactant protein D,SP-D)水平的影响,探讨噻托溴铵治疗COPD的新机制.方法 采用烟熏加气管内注入内毒素法建立大鼠COPD模型.将雄性SD大鼠随机分为对照组、COPD组和噻托溴铵干预组,每组10只.HE染色观察大鼠肺、支气管的病理改变,测定肺平均内衬间隔及平均肺泡数.酶联免疫吸附试验检测各组大鼠血清中SP D的含量.Western-blot及免疫组织化学方法检测大鼠肺组织中SP D的含量变化.结果 COPD组大鼠肺、支气管的HE染色符合COPD的病理改变；COPD组大鼠出现明显肺气肿,噻托溴铵组也出现肺气肿,但程度较轻.COPD组大鼠血清及肺组织中SP-D水平较对照组均明显升高(P＜0.01),噻托溴铵干预组与COPD组相比血清及肺组织中SP-D水平均显著降低(P＜0.01),但仍明显高于对照组(P＜0.01).结论 噻托溴铵可以降低COPD大鼠血清及肺组织中SP-D水平,这一作用可能是通过减轻气道炎症及肺气肿的程度来实现的.
目的 通過研究噻託溴銨對慢性阻塞性肺疾病(COPD)大鼠血清及肺組織中肺錶麵活性蛋白-D(pulmonary surfactant protein D,SP-D)水平的影響,探討噻託溴銨治療COPD的新機製.方法 採用煙熏加氣管內註入內毒素法建立大鼠COPD模型.將雄性SD大鼠隨機分為對照組、COPD組和噻託溴銨榦預組,每組10隻.HE染色觀察大鼠肺、支氣管的病理改變,測定肺平均內襯間隔及平均肺泡數.酶聯免疫吸附試驗檢測各組大鼠血清中SP D的含量.Western-blot及免疫組織化學方法檢測大鼠肺組織中SP D的含量變化.結果 COPD組大鼠肺、支氣管的HE染色符閤COPD的病理改變；COPD組大鼠齣現明顯肺氣腫,噻託溴銨組也齣現肺氣腫,但程度較輕.COPD組大鼠血清及肺組織中SP-D水平較對照組均明顯升高(P＜0.01),噻託溴銨榦預組與COPD組相比血清及肺組織中SP-D水平均顯著降低(P＜0.01),但仍明顯高于對照組(P＜0.01).結論 噻託溴銨可以降低COPD大鼠血清及肺組織中SP-D水平,這一作用可能是通過減輕氣道炎癥及肺氣腫的程度來實現的.
목적 통과연구새탁추안대만성조새성폐질병(COPD)대서혈청급폐조직중폐표면활성단백-D(pulmonary surfactant protein D,SP-D)수평적영향,탐토새탁추안치료COPD적신궤제.방법 채용연훈가기관내주입내독소법건립대서COPD모형.장웅성SD대서수궤분위대조조、COPD조화새탁추안간예조,매조10지.HE염색관찰대서폐、지기관적병리개변,측정폐평균내츤간격급평균폐포수.매련면역흡부시험검측각조대서혈청중SP D적함량.Western-blot급면역조직화학방법검측대서폐조직중SP D적함량변화.결과 COPD조대서폐、지기관적HE염색부합COPD적병리개변；COPD조대서출현명현폐기종,새탁추안조야출현폐기종,단정도교경.COPD조대서혈청급폐조직중SP-D수평교대조조균명현승고(P＜0.01),새탁추안간예조여COPD조상비혈청급폐조직중SP-D수평균현저강저(P＜0.01),단잉명현고우대조조(P＜0.01).결론 새탁추안가이강저COPD대서혈청급폐조직중SP-D수평,저일작용가능시통과감경기도염증급폐기종적정도래실현적.
Objeetive To investigate the new mechanism of tiotropium bromide in the treatment of chronic obstructive pulmonary disease(COPD)by studying its effect on pulmonary surfactant protein D(SP-D) in serum and lung tissue of COPD rats.Methods We established the rat COPD models by passive smoking as well as intratracheal instillation of Lipopolysaccharide respectively. Thirty male SD rats were randomly divicied into the control group,the COPD group and the tiotropium bromide treatment group (n=10).The pathologic change of the lung tissue and airway were observed by HE staining.The level of SPD in serum was measured by enzyme-linked immunosorbent assay.The level of SP-D in lung tissue was detected by western-blot and immunohistochemistry.Results HE staining proved that the rat COPD models were successfully established.The COPD group appears obvious emphysema,while the treatment group appears mild emphysema.The expression of SP-D in serum and lung tissue of the COPD group were obviously stronger than that in the control group(P<0.01).The level of SPD in serum and lung tissue of the treatment group was between the control group's and the COPD group's.Conclusions Tiotropium bromide could reduce the level of SP-D in serum and lung tissue of COPD rat.These effects may be exerted through alleviating airway inflammation and the degree of emphysema.
