国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2014年
1期
12-16
,共5页
慢性阻塞性肺疾病%诱导痰%α-防御素
慢性阻塞性肺疾病%誘導痰%α-防禦素
만성조새성폐질병%유도담%α-방어소
Chronic obstructive pulmonary disease%Induced sputum%Alpha defensins
目的 探讨慢性阻塞性肺疾病急性加重期(AECOPD)患者治疗前后诱导痰中α-防御素1-3(HNP1-3)含量、中性粒细胞比例(N％)与肺功能及血气分析结果的相关性,以探讨HNP1-3在COPD发病机制中的可能作用.方法 收集AECOPD患者42例(根据肺功能检测结果分为轻度组11例、中度组13例、重度组18例)治疗前后及20例急性支气管炎痊愈者(对照组)的诱导痰,分别进行痰中性粒细胞计数并计算其百分比,用ELISA方法检测诱导痰中HNP1-3的含量;测定各观察对象治疗前后的血气分析及肺功能,分析HNP1-3含量与N％、肺功能和血气分析的相关性.结果 COPD患者诱导痰中HNP1-3水平、N％、PaCO2随病情严重程度的增加而增高(P＜0.01),并明显高于对照组(P＜0.01),FEV1％ pred、FEV1/FVC、PaO2随病情严重程度的增加而降低(P＜0.01),明显低于对照组(P＜0.01).三组患者诱导痰中HNP1-3含量分别与N％呈显著正相关(r=0.887～0.973,P值均＜0.01),与FEV1％pred、FEV1/FVC、PaO2分别呈显著负相关(r=-0.721～-0.973,P值均＜0.01).经治疗一周后,轻度、中度、重度患者FEV1％ pred、FEV1/FVC、PaO2明显增高,诱导痰中HNP1-3含量、N％明显降低.结论 HNP1-3参与了COPD炎症的过程,此过程与中性粒细胞有关.痰中HNP1-3含量可作为COPD患者病情严重程度的指标,并有助于判断预后.
目的 探討慢性阻塞性肺疾病急性加重期(AECOPD)患者治療前後誘導痰中α-防禦素1-3(HNP1-3)含量、中性粒細胞比例(N％)與肺功能及血氣分析結果的相關性,以探討HNP1-3在COPD髮病機製中的可能作用.方法 收集AECOPD患者42例(根據肺功能檢測結果分為輕度組11例、中度組13例、重度組18例)治療前後及20例急性支氣管炎痊愈者(對照組)的誘導痰,分彆進行痰中性粒細胞計數併計算其百分比,用ELISA方法檢測誘導痰中HNP1-3的含量;測定各觀察對象治療前後的血氣分析及肺功能,分析HNP1-3含量與N％、肺功能和血氣分析的相關性.結果 COPD患者誘導痰中HNP1-3水平、N％、PaCO2隨病情嚴重程度的增加而增高(P＜0.01),併明顯高于對照組(P＜0.01),FEV1％ pred、FEV1/FVC、PaO2隨病情嚴重程度的增加而降低(P＜0.01),明顯低于對照組(P＜0.01).三組患者誘導痰中HNP1-3含量分彆與N％呈顯著正相關(r=0.887～0.973,P值均＜0.01),與FEV1％pred、FEV1/FVC、PaO2分彆呈顯著負相關(r=-0.721～-0.973,P值均＜0.01).經治療一週後,輕度、中度、重度患者FEV1％ pred、FEV1/FVC、PaO2明顯增高,誘導痰中HNP1-3含量、N％明顯降低.結論 HNP1-3參與瞭COPD炎癥的過程,此過程與中性粒細胞有關.痰中HNP1-3含量可作為COPD患者病情嚴重程度的指標,併有助于判斷預後.
목적 탐토만성조새성폐질병급성가중기(AECOPD)환자치료전후유도담중α-방어소1-3(HNP1-3)함량、중성립세포비례(N％)여폐공능급혈기분석결과적상관성,이탐토HNP1-3재COPD발병궤제중적가능작용.방법 수집AECOPD환자42례(근거폐공능검측결과분위경도조11례、중도조13례、중도조18례)치료전후급20례급성지기관염전유자(대조조)적유도담,분별진행담중성립세포계수병계산기백분비,용ELISA방법검측유도담중HNP1-3적함량;측정각관찰대상치료전후적혈기분석급폐공능,분석HNP1-3함량여N％、폐공능화혈기분석적상관성.결과 COPD환자유도담중HNP1-3수평、N％、PaCO2수병정엄중정도적증가이증고(P＜0.01),병명현고우대조조(P＜0.01),FEV1％ pred、FEV1/FVC、PaO2수병정엄중정도적증가이강저(P＜0.01),명현저우대조조(P＜0.01).삼조환자유도담중HNP1-3함량분별여N％정현저정상관(r=0.887～0.973,P치균＜0.01),여FEV1％pred、FEV1/FVC、PaO2분별정현저부상관(r=-0.721～-0.973,P치균＜0.01).경치료일주후,경도、중도、중도환자FEV1％ pred、FEV1/FVC、PaO2명현증고,유도담중HNP1-3함량、N％명현강저.결론 HNP1-3삼여료COPD염증적과정,차과정여중성립세포유관.담중HNP1-3함량가작위COPD환자병정엄중정도적지표,병유조우판단예후.
Objective To study the correlation among the levels of alpha defensin1-3 (HNP1-3),percentage of neutrophil (N％) in induced sputum and lung function as well as blood gas analysis in COPD patients with acute exacerbation at pre-and post-treatment,and to explore possible roles of HNP1-3 in COPD.Methods Forty-two patients COPD with acute exacerbation were recruited and randomly divided into three groups according to their lung function,twenty acute bronchitis recovery subjects also were selected.The variation of lung function,blood gas analysis,N％ and concentrations of HNP1-3 (by enzyme-linked immunosorbent assay) in induced sputum at pre-and post-treatment and control group were measured,the correlations among HNP1-3,N％ and lung function,blood gas analysis were analyzed.Results HNP1-3,N％ in induced sputum and PaCO2 in exacerbation of COPD were higher following higher severity of COPD(P ＜0.01),and remarkable higher compared to control group(P ＜0.01),while FEV1 ％pred,FEV1/FVC and PaO2 in exacerbation of COPD were lower following higher severity of COPD(P ＜0.01),and notable lower compared to control group (P ＜0.01).The HNP1-3 level in sputum was closely positively correlated with N％ (r =0.887 to 0.973,respectively P ＜ 0.05) and closely negatively correlated with FEV1 ％ pred,FEV1/FVC and PaO2 (r =-0.721 to-0.973,respectively P ＜ 0.05).There was remarkable improvement in FEV1 ％ pred,FEV1/FVC and PaO2 accompanied with notable decrease of N％ and concentration of HNP1-3 in induced sputum at mild/moderate/severe patients following treatment one week later.Conclusions HNP1-3 may be involved in the pathogenesis of airway inflammation in COPD.Sputum HNP1-3 may be a marker of severity of COPD,to some degree,be useful to predict prognosis.
