CAJ | 학술논문

目的研究血红素加氧酶-1(HO-1)对ALI大鼠的保护作用和对肺组织水通道蛋白1(AQP-1)、AQP-5表达的影响及可能的调节机制.方法 30只Wistar大鼠随机分成3组,即生理盐水组、ALI组和血红素组.尾静脉注射脂多糖后观察4 h,光镜下观察肺组织病理改变程度,测定肺组织湿/干重比值,检测大鼠血清中IL-8及环磷酸腺苷(cAMP)的含量;免疫组化方法观察肺组织AQP-1、AQP-5及HO-1的表达.结果 ①与生理盐水组相比,ALI组肺组织湿/干重比值显著升高(P＜0.001),血清IL-8含量升高(P＜0.05);血红素组肺组织湿/干重比值及IL-8含量均低于ALI组(P＜0.05),肺损伤炎症减轻.②血红素组AQP-1、AQP-5的表达均显著高于ALI组(P＜0.001).③ALI组大鼠血清cAMP含量与生理盐水组比较差异没有统计学意义,血红素组cAMP含量较ALI组增加(P＜0.05).结论 HO-1可以上调AQP-1、AQP-5的表达,减轻ALI时的肺水肿,可能是通过抑制炎症因子IL-8的表达实现的.cAMP可能参与了ALI时AQP-1与AQP-5的调节.
목적 연구혈홍소가양매-1(HO-1)대ALI대서적보호작용화대폐조직수통도단백1(AQP-1)、AQP-5표체적영향급가능적조절궤제.방법 30지Wistar대서수궤분성3조,즉생리염수조、ALI조화혈홍소조.미정맥주사지다당후관찰4 h,광경하관찰폐조직병리개변정도,측정폐조직습/간중비치,검측대서혈청중IL-8급배린산선감(cAMP)적함량;면역조화방법관찰폐조직AQP-1、AQP-5급HO-1적표체.결과 ①여생리염수조상비,ALI조폐조직습/간중비치현저승고(P＜0.001),혈청IL-8함량승고(P＜0.05);혈홍소조폐조직습/간중비치급IL-8함량균저우ALI조(P＜0.05),폐손상염증감경.②혈홍소조AQP-1、AQP-5적표체균현저고우ALI조(P＜0.001).③ALI조대서혈청cAMP함량여생리염수조비교차이몰유통계학의의,혈홍소조cAMP함량교ALI조증가(P＜0.05).결론 HO-1가이상조AQP-1、AQP-5적표체,감경ALI시적폐수종,가능시통과억제염증인자IL-8적표체실현적.cAMP가능삼여료ALI시AQP-1여AQP-5적조절.
Objective To research the protective effects of heme oxygenase-1 (HO-1) on ALI rats,the influence of HO-1 on the expression of aquaporin-1 (AQP-1) and AQP-5 in lung tissue, and the possible mechanism. Methods Thirty Wistar rats were randomly divided into three groups:normal saline treated group,ALI model group,and hemin induced group.The rats were observed for four hours after injecting lipopolysaccharide in tail vein,the optical microscope was used to observe the pathological changes of lung tissue,wet/dry ratio in lung tissue was measured, and the content of IL-8 and cAMP in rat serum was determined. The immunohistochemical method was used to detect the expressions of AQP-1,AQP-5,and HO-1 in lung tissue.Results ①Compared with normal saline treated group, the wet/dry ratio increased significantly in ALI model group (P＜0.001), and the IL-8 level in serum in ALI model group was also higher than that in normal saline treated group (P＜0.05).The wet/dry ratio and IL-8 level in serum in hemin induced group were lower than those in ALI model group (P＜0.05),and the inflammation of lung injury was reduced.②The expressions of AQP-1 and AQP-5 in hemin induced group were significantly higher than those in ALI model group (P＜0.001).③There was no statistical significance on the content of cAMP between ALI model group and normal saline treated group.The content of cAMP in hemin induced group was higher than that in ALI model group (P＜0.05).Conclusions HO-1 may upregulate the expressions of AQP-1 and AQP-5,reduce the degrees of pulmonary edema in ALI rats possibly through inhibiting the expression of inflammatory factor IL-8 .cAMP may be involved in the regulation of AQP-1 and AQP-5 in ALI.