国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2014年
5期
325-327
,共3页
特异性免疫治疗%过敏性哮喘%白介素10%转化生长因子β1%室尘螨
特異性免疫治療%過敏性哮喘%白介素10%轉化生長因子β1%室塵螨
특이성면역치료%과민성효천%백개소10%전화생장인자β1%실진만
Specific immunotherapy%Allergic asthma%Interleukin-10%Transforming growth factor-β1%House dust mites
目的 本文通过观察对室尘螨(HDM)过敏的儿童哮喘患者过敏原特异性免疫治疗(allergen specific immunotherapy,SIT)后外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中IL-10、转化生长因子β1(TGF-β1)表达情况和血清中二者的含量变化,初步探讨儿童过敏性哮喘SIT的机制,从而为哮喘的防治提供新思路.方法 选取郑州大学第一附属医院儿科门诊48例儿童过敏性哮喘患者,分两组,对哮喘组进行标准化SIT 1.5～2年后,采集两组患者的外周静脉血.利用不同的方法检测每组标本PBMCs中IL-10和TGF-β1mRNA以及血清中二者的表达情况,并进行统计学分析.结果 HDM-SIT组中IL-10 mRNA的相对表达量显著升高,是哮喘组的3.29倍,TGF-β1mRNA的相对表达量在HDM-SIT组稍升高,是哮喘组的1.56倍.ELISA检测结果发现与哮喘组比较,HDM-SIT组血清中IL-10和TGF-β1的含量明显升高.结论 本实验进一步证实了哮喘患者SIT有效.在HDM-SIT组IL-10和TGF-β1的表达显著升高,提示二者可能在儿童过敏性哮喘SIT中起重要作用.
目的 本文通過觀察對室塵螨(HDM)過敏的兒童哮喘患者過敏原特異性免疫治療(allergen specific immunotherapy,SIT)後外週血單箇覈細胞(peripheral blood mononuclear cells,PBMCs)中IL-10、轉化生長因子β1(TGF-β1)錶達情況和血清中二者的含量變化,初步探討兒童過敏性哮喘SIT的機製,從而為哮喘的防治提供新思路.方法 選取鄭州大學第一附屬醫院兒科門診48例兒童過敏性哮喘患者,分兩組,對哮喘組進行標準化SIT 1.5～2年後,採集兩組患者的外週靜脈血.利用不同的方法檢測每組標本PBMCs中IL-10和TGF-β1mRNA以及血清中二者的錶達情況,併進行統計學分析.結果 HDM-SIT組中IL-10 mRNA的相對錶達量顯著升高,是哮喘組的3.29倍,TGF-β1mRNA的相對錶達量在HDM-SIT組稍升高,是哮喘組的1.56倍.ELISA檢測結果髮現與哮喘組比較,HDM-SIT組血清中IL-10和TGF-β1的含量明顯升高.結論 本實驗進一步證實瞭哮喘患者SIT有效.在HDM-SIT組IL-10和TGF-β1的錶達顯著升高,提示二者可能在兒童過敏性哮喘SIT中起重要作用.
목적 본문통과관찰대실진만(HDM)과민적인동효천환자과민원특이성면역치료(allergen specific immunotherapy,SIT)후외주혈단개핵세포(peripheral blood mononuclear cells,PBMCs)중IL-10、전화생장인자β1(TGF-β1)표체정황화혈청중이자적함량변화,초보탐토인동과민성효천SIT적궤제,종이위효천적방치제공신사로.방법 선취정주대학제일부속의원인과문진48례인동과민성효천환자,분량조,대효천조진행표준화SIT 1.5～2년후,채집량조환자적외주정맥혈.이용불동적방법검측매조표본PBMCs중IL-10화TGF-β1mRNA이급혈청중이자적표체정황,병진행통계학분석.결과 HDM-SIT조중IL-10 mRNA적상대표체량현저승고,시효천조적3.29배,TGF-β1mRNA적상대표체량재HDM-SIT조초승고,시효천조적1.56배.ELISA검측결과발현여효천조비교,HDM-SIT조혈청중IL-10화TGF-β1적함량명현승고.결론 본실험진일보증실료효천환자SIT유효.재HDM-SIT조IL-10화TGF-β1적표체현저승고,제시이자가능재인동과민성효천SIT중기중요작용.
Objective The aim of this study was to house dust mite (HDM) children asthma allergen specific immunotherapy (SIT) of allergy of peripheral blood mononuclear cells (PBMCs) changes in IL-10,TGF-β1 expression and the serum content of two,preliminary study on the mechanism of allergic asthma in children with SIT,so as to provide new ideas for prevention and treatment of asthma.Methods The outpatient department of Pediatrics The First Affiliated Hospital of Zhengzhou University in 48 children with allergic asthma patients were divided into two groups,one group was normalized to SIT 1.5-2 years later,peripheral venous blood collected from two groups of patients.Expression of two using different methods for detection of II-10 and TGF-β group of specimens of PBMCs 1 mRNA and serum,and statistical analysis.Results The relative expression level of IL-10 in group HDM-SIT,mRNA increased significantly,is 3.29 times the asthmatic group,the relative expression of TGF-β1 mRNA was slightly increased in HDM-SIT group,is 1.56 times that of asthma group.ELISA test results found that,compared with the asthmatic group,the contents of IL-10 and TGF-β1 HDM-SIT in serum increased significantly.Conclusions This study further confirmed the asthma patients SIT significantly increased in HDM-SIT group; the expression of IL-10 and TGF-β1,TGF-β1,suggesting that IL-10 may play an important role in allergic asthma in children.
